Abstract：
:Nucleos(t)ide analogues that inhibit hepatitis B virus (HBV) DNA replication are typically used as monotherapy for chronically infected patients. Treatment with a nucleos(t)ide analogue eliminates most HBV DNA replication intermediates and produces a gradual decline in levels of covalently closed circular DNA (cccDNA), the template for viral RNA synthesis. It remains uncertain if levels of cccDNA decline primarily through hepatocyte death, or if loss also occurs during hepatocyte mitosis. To determine if cccDNA survives mitosis, growing ducklings infected with duck hepatitis B virus (DHBV) were treated with the nucleoside analogue, Entecavir. Viremia was suppressed at least 10(5)-fold, during a period when average liver mass increased 23-fold. Analysis of the data suggested that if cccDNA synthesis was completely inhibited, at least 49% of cccDNA survived hepatocyte mitosis. However, there was a large duck-to-duck variation in cccDNA levels, suggesting that low level cccDNA synthesis may contribute to this apparent survival through mitosis.
journal_name
Virologyjournal_title
Virologyauthors
Reaiche-Miller GY,Thorpe M,Low HC,Qiao Q,Scougall CA,Mason WS,Litwin S,Jilbert ARdoi
10.1016/j.virol.2013.08.014subject
Has Abstractpub_date
2013-11-01 00:00:00pages
357-64issue
1-2eissn
0042-6822issn
1096-0341pii
S0042-6822(13)00487-Xjournal_volume
446pub_type
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