Abstract:
:Induction with ultraviolet light of K12 lysogens carrying defective prophages leads to death of the host cell. In order to determine whether the killing accompanying induction was due to diffusible phage products, or was a result of prophage excision itself, the survival of lysogens carrying DNA defective prophages on an extra chromosomal site (the F-gal episome), were examined. Induction of prophages, carrying mutations in the x region, or the N, O or P cistrons, from this location, did not result in host death, but, with the exception of mutants in the x region of the lambda chromosome, it did result in loss of the episome. On the basis of these results, it is proposed that the lethal event in induction of the DNA-defective mutants is due to prophage excision, which leads to interruption of the host chromosome, and cell death.
journal_name
Virologyjournal_title
Virologyauthors
Eisen HA,Siminovitch L,Mohide PTdoi
10.1016/0042-6822(68)90012-3subject
Has Abstractpub_date
1968-01-01 00:00:00pages
97-103issue
1eissn
0042-6822issn
1096-0341pii
0042-6822(68)90012-3journal_volume
34pub_type
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