Abstract：
:Porcine circovirus type 2 (PCV2) is the causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome. In this work, the RNAs of PCV2 synthesized during productive infection in porcine kidney cells were characterized. A total of nine RNAs were detected. They include the viral capsid protein RNA (CR), a cluster of five Rep-associated RNAs (designated Rep, Rep', Rep3a, Rep3b, and Rep3c), and three NS-associated RNA (designated NS515, NS672, and NS0). Members of the Rep-associated RNA cluster all share common 5' and 3' nucleotide sequences and they also share 200 common 3' nucleotides with the NS-associated RNAs. Rep, capable of coding for the replication-associated-protein (RepP), appears to be the primary transcript that gives rise to Rep', Rep3a, Rep3b, and Rep3c by alternate splicing. Protein sequence alignment showed that RepP and the Rep' protein of PCV2 are equivalent to those described for PCV type 1 (PCV1) (a nonpathogenic virus), which had been shown to be essential for viral DNA replication. The results also suggest that NS515, NS672, and NS0 are transcribed from three different promoters inside ORF1 downstream of the Rep promoter. To date, only three RNAs (CR, Rep, and Rep') have been reported for PCV1-infected porcine kidney cells. Therefore, it is important to apply similar strategies from this study to reexamine the transcription pattern of PCV1.
journal_name
Virologyjournal_title
Virologyauthors
Cheung AKdoi
10.1006/viro.2002.1733subject
Has Abstractpub_date
2003-01-05 00:00:00pages
168-80issue
1eissn
0042-6822issn
1096-0341pii
S0042682202917332journal_volume
305pub_type
杂志文章相关文献
VIROLOGY文献大全abstract：:Without a virus culture system, genetic analysis becomes the principal method to classify norovirus (NoV) strains. Currently, classification of NoV strains beneath the species level has been based on sequences from different regions of the viral genome. As a result, the phylogenetic insights of some virus were not app...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2005.11.015
更新日期：2006-03-15 00:00:00
abstract：:Rotaviruses are the leading cause of severe diarrhea in infants and young children worldwide. Thus, the development of an effective rotavirus vaccine is a major public health goal. This study was performed to identify the gene or genes responsible for rotavirus virulence or host range restriction and attenuation in a ...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1995.1255
更新日期：1995-05-10 00:00:00
abstract：:During Herpes simplex virus envelopment, capsids, tegument polypeptides, and membrane proteins assemble at the site of budding and a cellular lipid bilayer becomes refashioned into a spherical envelope. Though the molecular interactions driving these events are poorly understood, several lines of evidence suggest that...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2003.08.023
更新日期：2003-12-05 00:00:00
abstract：:Marek's disease virus (MDV) is an oncogenic alphaherpesvirus of Gallus gallus, the domesticated chicken. Control strategies rely upon vaccination with live attenuated viruses of antigenically similar avian herpesviruses or attenuated strains of MDV. Recent studies in other viruses have shown that recoding certain vira...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2018.01.020
更新日期：2018-03-01 00:00:00
abstract：:Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since R...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2012.11.020
更新日期：2013-03-30 00:00:00
abstract：:Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(85)90283-1
更新日期：1985-07-30 00:00:00
abstract：:The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epith...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2015.02.054
更新日期：2015-08-01 00:00:00
abstract：:Three prophage sequences were identified in the Lactobacillus johnsoni strain NCC533. Prophage Lj965 predicted a gene map very similar to those of pac-site Streptococcus thermophilus phages over its DNA packaging and head and tail morphogenesis modules. Sequence similarity linked the putative DNA packaging and head mo...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.2000.0498
更新日期：2000-09-30 00:00:00
abstract：:Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies. Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215. Substitution of alanine for threonine at this position reduced early ...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2008.10.002
更新日期：2009-01-05 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 is targeted by broadly-reactive neutralizing antibodies 2F5 and 4E10, making it an attractive target for vaccine development. To better assess immunogenic properties of gp41, we generated five soluble glutathione S-transferase fusion proteins ...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2007.11.009
更新日期：2008-03-15 00:00:00
abstract：:Plant virus-based vectors provide valuable tools for expression of foreign proteins in plants and for gene function studies. None of the presently available virus vectors is suitable for use in soybean. In the present study, we produced Bean pod mottle virus (BPMV)-based vectors that are appropriate for gene expressio...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2005.08.046
更新日期：2006-01-20 00:00:00
abstract：:Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2015.04.022
更新日期：2015-09-01 00:00:00
abstract：:Astrocytes protect neurons but also evoke a proinflammatory response to injury and viral infections including HIV. We investigated the mechanism of HIV-1 infection in primary astrocytes, which showed minimal but productive viral infection independent of CXCR4. As with ectopic-CD4-expressing astrocytes, lysosomotropic ...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2014.03.002
更新日期：2014-05-01 00:00:00
abstract：:The sequence of gene VI from figwort mosaic virus (FMV) clone x4 was determined and compared with that previously published for FMV clone DxS. Both clones originated from the same virus isolation, but the virus used to clone DxS was propagated extensively in a host of a different family prior to cloning whereas that u...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(91)90624-k
更新日期：1991-06-01 00:00:00
abstract：:The hemagglutinin of the 2009 pandemic H1N1 influenza virus is a derivative of and is antigenically related to classical swine but not to seasonal human H1N1 viruses. We compared the A/California/7/2009 (CA/7/09) virus recommended by the WHO as the reference virus for vaccine development, with two classical swine infl...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2010.09.009
更新日期：2010-12-05 00:00:00
abstract：:Sequential isolates of caprine arthritis-encephalitis lentivirus (CAEV) were obtained from clinically arthritic carpal joints of three Saanen goats between 36.4 and 44.9 months after infection with biologically cloned CAEV isolate 63. These virus isolates and the CAEV-63 inoculum were evaluated for neutralization by a...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1993.1542
更新日期：1993-10-01 00:00:00
abstract：:The expression and subcellular location of the 33.6-kDa nonstructural protein NSm of tomato spotted wilt virus (TSWV) was analyzed in Nicotiana rustica plants and protoplasts as a function of time. Immunofluorescent studies in protoplasts isolated from TSWV-infected N. rustica leaves showed that this protein could fir...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1995.0059
更新日期：1995-12-20 00:00:00
abstract：:Propagation of human influenza viruses in embryonated chicken eggs (CE) results in the selection of variants with amino acid substitutions near the receptor-binding site of the hemagglutinin (HA) molecule. To evaluate the mechanisms by which these substitutions enable human virus growth in CE, we studied the binding o...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1999.9732
更新日期：1999-06-05 00:00:00
abstract：:Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in the virus family Bunyaviridae. Nucleocapsid RNA from Hantaan virions and a control bunyavirus were examined for ribonuclease A (RNase A) se...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(83)90514-7
更新日期：1983-12-01 00:00:00
abstract：:Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analy...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2010.04.009
更新日期：2010-09-01 00:00:00
abstract：:The induction of autoimmunity by viruses has been hypothesized to occur by a number of mechanisms. Coxsackievirus B4 (CB4) induces hyperglycemia in SJL mice resembling diabetes in humans. While virus is effectively cleared within 2 weeks, hyperglycemia does not appear until about 8-12 weeks postinfection at a time whe...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/s0042-6822(03)00462-8
更新日期：2003-09-30 00:00:00
abstract：:An in-frame gag gene mutant of human immunodeficiency virus type 1, which carries two amino acid substitutions in the center of the p24 coding region, was constructed in vitro, and its replication properties in several cell lines were examined. In CD4-negative SW480 cells transfected with the mutant clone, synthesis a...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1995.1478
更新日期：1995-09-10 00:00:00
abstract：:Geographical separation of host species has shaped the avian influenza A virus gene pool into independently evolving Eurasian and American lineages, although phylogenetic evidence for gene flow and reassortment indicates that these lineages also mix on occasion. While the evolutionary dynamics of the avian influenza g...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2009.05.002
更新日期：2009-08-01 00:00:00
abstract：:Coinfection of tobacco plants with potato virus X (PVX) and any of several members of the potyvirus group causes a synergistic disease characterized by a dramatic increase in symptom severity correlated with a 3- to 10-fold increase in the accumulation of PVX in the first systemically infected leaves. We have recently...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1997.8488
更新日期：1997-04-28 00:00:00
abstract：:Human nectin-1 (HveC, Prr1), a member of the immunoglobulin superfamily and a receptor for the entry of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1), binds to viral gD. For HSV-1, HSV-2, and PRV, the gD-binding region of nectin-1 has been localized to the N-...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.2001.0989
更新日期：2001-07-05 00:00:00
abstract：:The synthesis of linear duplex replicative structures (monomers, head-to-head, and tail-to-tail dimers) is an important hallmark of the productive phase of the adeno-associated virus (AAV) life cycle. These structures are generated by a strand-displacement replication mechanism and believed to be a reservoir for singl...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.2000.0504
更新日期：2000-09-30 00:00:00
abstract：:In 1943 Burnet reported on changes in the hemagglutinating properties of human influenza virus which occurred during adaptation of the virus to growth in chicken eggs. Only recently has direct evidence been presented that these changes affect the antigenic properties of the virus. Schild et al. (G. C. Schild, J. S. Ox...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(85)90105-9
更新日期：1985-05-01 00:00:00
abstract：:The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) rais...
journal_title：Virology
pub_type： 杂志文章
doi：10.1006/viro.1994.1427
更新日期：1994-08-01 00:00:00
abstract：:Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cy...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/j.virol.2018.12.005
更新日期：2019-02-01 00:00:00
abstract：:The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...
journal_title：Virology
pub_type： 杂志文章
doi：10.1016/0042-6822(92)90094-6
更新日期：1992-01-01 00:00:00
