Abstract:
:Posttranslational modification of viral proteins by cellular enzymes is a feature of many virus replication strategies. Here, we report that during infection the multifunctional human influenza A virus NS1 protein is phosphorylated at threonine-215. Substitution of alanine for threonine at this position reduced early viral propagation, an effect apparently unrelated to NS1 antagonizing host interferon responses or activating phosphoinositide 3-kinase signaling. In vitro, a subset of cellular proline-directed kinases, including cyclin dependent kinases (CDKs) and extracellular signal-regulated kinases (ERKs), potently phosphorylated NS1 protein at threonine-215. Our data suggest that CDK/ERK-mediated phosphorylation of NS1 at threonine-215 is important for efficient virus replication.
journal_name
Virologyjournal_title
Virologyauthors
Hale BG,Knebel A,Botting CH,Galloway CS,Precious BL,Jackson D,Elliott RM,Randall REdoi
10.1016/j.virol.2008.10.002subject
Has Abstractpub_date
2009-01-05 00:00:00pages
6-11issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(08)00657-0journal_volume
383pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The satellite RNA of Bamboo mosaic virus (satBaMV) contains on open reading frame for a 20-kDa protein that is flanked by a 5'-untranslated region (UTR) of 159 nucleotides (nt) and a 3'-UTR of 129 nt. A secondary structure was predicted for the 5'-UTR of satBaMV RNA, which folds into a large stem-loop (LSL) and a smal...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00178-8
更新日期:2003-06-20 00:00:00
abstract::The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6-methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8936
更新日期:1998-01-20 00:00:00
abstract::Seven structural component proteins of a paramyxovirus simian virus 41 (SV41) were identified with the aid of monoclonal antibodies prepared against SV41 and human parainfluenza type 2 virus (PIV2). The nucleoprotein is antigenically very close to that of PIV2, while it is comparatively far from that of simian virus 5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90141-d
更新日期:1990-12-01 00:00:00
abstract::The simian virus 40 (SV40) large T antigen was used as an immunogenic vector to express a herpes simplex virus type 1 (HSV-1) glycoprotein B (gB), H-2Kb-restricted cytotoxic T lymphocyte (CTL) recognition epitope corresponding to amino acid residues 498-505. Immunization of naive, C57BL/6 mice with a cell line, B6/350...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1657
更新日期:1993-12-01 00:00:00
abstract::Two major infectious forms of vaccinia virus (VACV) have been described: the intracellular mature virion (IMV), and the extracellular enveloped virion (EEV). Due to their stability in the environment, IMVs play a predominant role in host-to-host transmission, whereas EEVs play an important role in dissemination within...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00038-7
更新日期:2003-02-01 00:00:00
abstract::The requirement to replicate in both vertebrate and invertebrate hosts is thought to limit the introduction of genetic changes into the genome of arboviruses. Serial passage under laboratory conditions will overcome this limitation allowing for genetic changes to be introduced and affecting the virulence of the virus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.03.030
更新日期:2011-06-20 00:00:00
abstract::Expansion of CHIKV outbreaks with appearance of novel mutations are reported from many parts of the world. Two novel mutations viz. E1:K211E and E2:V264A in background of E1:226A are recently identified from Aedes aegypti dominated areas of India. In this study, the role of these mutations in modulation of infectivity...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.025
更新日期:2016-10-01 00:00:00
abstract::The transcriptive complex, the nucleocapsid with the viral RNA-synthesizing activity, of Newcastle disease virus (NDV) contains three protein components, the major structural subunit (NP) and two associated proteins (P and L) involved in the RNA synthesis. We studied the pathway of these proteins from synthesis to ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90132-1
更新日期:1985-12-01 00:00:00
abstract::We have compared the deduced envelope (E) protein sequences of two biologically well-characterized yellow fever (YF) virus vaccine strains. The 17DD strain has been produced in Brazil for more than 50 years and used to successfully vaccinate millions of people worldwide. The 17D-213 is a candidate vaccine strain produ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90745-b
更新日期:1992-05-01 00:00:00
abstract::Bovine respiratory disease (BRD) is the costliest disease affecting the cattle industry globally. Orthomyxoviruses, influenza C virus (ICV) and influenza D virus (IDV) have recently been implicated to play a role in BRD. However, there are contradicting reports about the association of IDV and ICV to BRD. Using the la...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.08.014
更新日期:2020-12-01 00:00:00
abstract::We report the isolation and characterization of HEp-2c cell clones obtained after two successive persistent poliovirus (PV) infections. Once cured, some of the cell clones displayed selective permissivity toward the wild-type Mahoney strain and partial resistance to particular mutants of this strain, including the Sab...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1488
更新日期:1995-10-01 00:00:00
abstract::Identification of regulators of p53 expression is a crucial step in understanding the diverse functions of p53 and its role in cellular homeostasis and responsiveness to insult. Several viral proteins inactivate p53 as a modulator of cell cycle progression and apoptosis. Here, we report that a unique leporipoxviral tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0507
更新日期:1996-10-01 00:00:00
abstract::Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90017-j
更新日期:1992-02-01 00:00:00
abstract::A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90560-e
更新日期:1990-08-01 00:00:00
abstract::The host innate response to viral infection includes the production of interferons, which is dependent on the coordinated activity of multiple transcription factors. Herpes simplex virus 1 (HSV-1) has been shown to block efficient interferon expression by multiple mechanisms. We and others have demonstrated that HSV-1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.028
更新日期:2007-04-10 00:00:00
abstract::Dendrolimus punctatus tetravirus (DpTV) belongs to the genus omegatetravirus of the Alphatetraviridae family. Sequence analysis predicts that DpTV replicase contains a putative helicase domain (Hel). However, the helicase activity in alphatetraviruses has never been formally determined. In this study, we determined th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.08.045
更新日期:2012-11-25 00:00:00
abstract::Nipah virus (NiV) causes severe encephalitis in humans, with high mortality. NiV nonstructural C protein (NiV-C) is essential for its pathogenicity, but its functions are unclear. In this study, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.013
更新日期:2016-10-01 00:00:00
abstract::Polyomaviral vectors are generated by transfecting 293T cells with three sets of DNAs: DNA for the expression of simian virus 40 (SV40) T antigen; DNA for the expression of SV40 capsid proteins, and vector DNA harboring a reporter gene expression cassette carrying a SV40 origin. The vector DNA harbors a minimal sequen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.032
更新日期:2008-09-15 00:00:00
abstract::Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8329
更新日期:1997-01-20 00:00:00
abstract::Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.08.006
更新日期:2005-12-05 00:00:00
abstract::The expression of two small basic proteins (C and C') encoded by a second open reading frame of the New Jersey serotype of vesicular stomatitis virus (VSV) P gene was reported previously (Spiropoulou and Nichol, J. Virol., 67, 3103-3110, 1993). Here we found that the Indiana serotype virus also expressed C and C' prot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0066
更新日期:1996-02-15 00:00:00
abstract::Monoclonal antibodies to the matrix or E1 glycoprotein of mouse hepatitis virus (MHV) were tested for their ability to protect mice from a normally lethal challenge of MHV-4. Four antibodies were tested, and two of these, J.1.3 and J.3.9, were protective. Protection did not correlate with virus neutralization in vitro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90415-7
更新日期:1989-01-01 00:00:00
abstract::Analysis of expression of the Friend murine leukemia virus (F-MuLV) and of the spleen focus forming virus (SFFV) has been undertaken in highly malignant interferon (IFN)-sensitive (745) and IFN-resistant (3Cl-8) Friend leukemia cells (FLC), serially passaged intraperitoneally in DBA/2 mice. In vivo passaged 745 cells,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90304-1
更新日期:1986-04-30 00:00:00
abstract::The avian hematopoietic system has long been an invaluable model to study the mechanisms of cell growth and differentiation. We have developed six MAbs against either chicken embryonic hematopoietic precursor cells or retrovirus-transformed cells. MAbs Mo1, Mo2, and Mo3 recognized transformation-associated markers exp...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90581-9
更新日期:1992-08-01 00:00:00
abstract::Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90139-3
更新日期:1983-10-15 00:00:00
abstract::We mutagenized and characterized a 41-kilobase-pair subgenomic cloned fragment from the unique long (UL) region of pseudorabies virus (PRV). Forty mutant clones, each carrying a single inserted oligonucleotide, were used for cotransfection with overlapping cloned viral DNA fragments. More than half of these transfecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1242
更新日期:1994-05-01 00:00:00
abstract::The P670 promoter of HPV16 directs transcription of the virus late genes in the differentiating epithelium. We found that CCAAT/enhancer binding protein beta (C/EBPbeta), a key transcription factor that induces the terminal differentiation of keratinocytes, enhanced the P670-driven transcription in transient reporter ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.025
更新日期:2006-03-01 00:00:00
abstract::The virulence of herpes simplex virus-1 (HSV-1) strains by the intraperitoneal (ip) route of injection in mice depends on the presence of an intact sequence in the HpaI DNA fragment P within coordinates 0.762 to 0.787. Deletion of the HpaI-P region (e.g., strain HFEM) abrogates the ability of the virus to infect mice ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90128-5
更新日期:1986-03-01 00:00:00
abstract::Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or -LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnucle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90027-o
更新日期:1990-06-01 00:00:00
abstract::The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.10.049
更新日期:2005-02-05 00:00:00