Differential accumulation of genetic and phenotypic changes in Venezuelan equine encephalitis virus and Japanese encephalitis virus following passage in vitro and in vivo.

abstract：:The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames (ORFs) that encode putative viral proteins. ORFs 2 to 7 were cloned in plasmids downstream of the Sp6 RNA polymerase promoter, and the translation of transcripts generated in vitro yielded proteins tha...

journal_title：Virology

authors： Meulenberg JJ,Petersen-den Besten A,De Kluyver EP,Moormann RJ,Schaaper WM,Wensvoort G

更新日期：1995-01-10 00:00:00

abstract：:Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...

journal_title：Virology

authors： Shapka N,Stork J,Nagy PD

更新日期：2005-12-05 00:00:00

abstract：:Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both ...

journal_title：Virology

authors： Marchi A,Nicoletti L,Accardi L,Di Bonito P,Giorgi C

更新日期：1998-06-20 00:00:00

abstract：:The glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) serves as virus attachment protein to its receptor on host cells and is a key determinant for cell tropism, pathogenesis, and epidemiology of the virus. The GP of LCMV is posttranslationally cleaved by the subtilase SKI-1/S1P into two subunits, the per...

journal_title：Virology

authors： Kunz S,Edelmann KH,de la Torre JC,Gorney R,Oldstone MB

更新日期：2003-09-15 00:00:00

abstract：:Hantaviruses are enveloped RNA viruses that belong to the family Bunyaviridae. They are the causative agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantaviruses show a worldwide distribution with specific rodent species as natural hosts. It is known that rodents can tr...

journal_title：Virology

authors： Muranyi W,Kehm R,Bahr U,Müller S,Handermann M,Darai G,Zeier M

更新日期：2004-01-05 00:00:00

abstract：:The 85-kDa polypeptide previously shown to be associated with the restricted (R) component of the EBV-induced early antigen (EA) complex was subjected to amino acid sequencing analysis. This was accomplished by cyanogen bromide cleavage and by separation of individual peptides by high-pressure liquid chromatography em...

journal_title：Virology

authors： Goldschmidts W,Luka J,Pearson GR

更新日期：1987-03-01 00:00:00

abstract：:Mammalian orthoreoviruses (reoviruses) are highly useful models for studies of double-stranded RNA virus replication and pathogenesis. We previously developed a strategy to recover prototype reovirus strain T3D from cloned cDNAs transfected into murine L929 fibroblast cells. Here, we report the development of a second...

journal_title：Virology

authors： Kobayashi T,Ooms LS,Ikizler M,Chappell JD,Dermody TS

更新日期：2010-03-15 00:00:00

abstract：:The ability of HIV to match levels of viral mRNA to the activation state of the host cell may play a role in its ability to persist as well as to replicate. This linkage depends on the function of the viral transcriptional regulatory protein, Tat, which increases the efficiency of RNA elongation (transcriptional proce...

journal_title：Virology

authors： Adams M,Wong C,Wang D,Romeo J

更新日期：1999-05-10 00:00:00

abstract：:Glycoprotein C (gC) of pseudorabies virus, a swine herpesvirus, initiates virus attachment by binding to heparan sulfate (HS) linked to proteoglycans (HSPGs) on the cell surface. This interaction facilitates a required step in virus entry, the binding to a non-HS coreceptor, likely by another viral glycoprotein, gD. W...

journal_title：Virology

authors： Rue CA,Ryan P

更新日期：2003-03-01 00:00:00

abstract：:Processing of the transmembrane glycoprotein (GP) of Marburg virus involved the conversion of an endo H-sensitive, ER-specific form into an endo H-resistant, Golgi-specific precursor that was cleaved into GP(1) and GP(2). Cleavage was mediated by furin or another subtilisin-like endoprotease with similar substrate spe...

journal_title：Virology

authors： Volchkov VE,Volchkova VA,Ströher U,Becker S,Dolnik O,Cieplik M,Garten W,Klenk HD,Feldmann H

更新日期：2000-03-01 00:00:00

abstract：:Several VV structural proteins are produced by the removal of amino-terminal peptides from their cognate precursors. In the experiments reported here, directed genetic approaches were used to investigate the possible role of these terminal peptides in protein processing. As a model system, the FLAG epitope-tagged P25K...

journal_title：Virology

authors： Lee P,Hruby DE

更新日期：1995-02-20 00:00:00

abstract：:The elucidation of the antigenic structure of the S glycoprotein of murine coronaviruses will provide further understanding of the complex pathogenicity of these viruses. In order to identify linear antigenic determinants, the primary structure of the S glycoprotein of murine hepatitis virus strain A59 was analyzed wi...

journal_title：Virology

authors： Daniel C,Lacroix M,Talbot PJ

更新日期：1994-08-01 00:00:00

abstract：:In transient gene expression assays we observed an increase in expression of the bacterial chloramphenicol acetyl-transferase (CAT) gene, under the transcriptional control of the HIV-1 LTR (pLTR-CAT), when this plasmid was cotransfected into Vero or MRC-5 cells with a plasmid containing either the HCMV immediate early...

journal_title：Virology

authors： Rando RF,Srinivasan A,Feingold J,Gonczol E,Plotkin S

更新日期：1990-05-01 00:00:00

abstract：:Hematopoietic tissues obtained from avian leukosis virus (ALV)-infected Hyline SC chickens were analyzed for the presence of integrated viral DNA sequences. Cells were prepared from bone marrow, bursa, spleen, thymus, and peripheral blood. Following the removal of erythrocytes, cellular DNAs from each of these tissues...

journal_title：Virology

authors： Baba TW,Humphries EH

更新日期：1986-12-01 00:00:00

abstract：:Mutants of adenovirus type 5 (Ad5) that lack early region 4 (E4) are defective in the expression of viral late genes. E4 mutants exhibit dramatically reduced levels of both cytoplasmic and nuclear viral late RNAs compared to wild-type virus, due principally to reduced stability of unprocessed viral late RNA in the nuc...

journal_title：Virology

authors： Sandler AB,Ketner G

更新日期：1991-03-01 00:00:00

abstract：:The primer binding site (PBS) plays pivotal roles during reverse transcription of retroviruses and also is the target of a cellular host defense impeding the transcription of murine leukemia virus (MLV) harboring a proline (pro) PBS in embryonic cells. Both the PBS and the tRNA primer are copied during reverse transcr...

journal_title：Virology

authors： Modin C,Lund AH,Schmitz A,Duch M,Pedersen FS

更新日期：2000-12-20 00:00:00

abstract：:In vitro translation of pH 8-washed particles of tobacco mosaic virus (TMV; vulgare, common, or U1 strain) in a cell-free system derived from Escherichia coli (MRE 600) results in a broad size range of TMV RNA-encoded products, including significant amounts of the distinctive 126-kDa polypeptide which is encoded by th...

journal_title：Virology

authors： Wilson TM

更新日期：1986-07-15 00:00:00

abstract：:We developed a positive selection method for recovering Marek's disease virus (MDV) recombinants. The Escherichia coli xanthine-guanine phosphoribosyltransferase gene (gpt), under the control of the major immediate-early promoter from cytomegalovirus, was inserted into the inverted repeats flanking the unique long (UL...

journal_title：Virology

authors： Marshall DR,Reilly JD,Liu X,Silva RF

更新日期：1993-08-01 00:00:00

abstract：:The PstI-N genomic fragment of Autographa californica nuclear polyhedrosis virus (AcNPV) encodes an immediate-early protein, IE2, that functions as a promiscuous transactivator of other early viral promoters and heterologous promoters both in vivo and in vitro. IE2 contains several sequence motifs that are common to t...

journal_title：Virology

authors： Yoo S,Guarino LA

更新日期：1994-07-01 00:00:00

abstract：:Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...

journal_title：Virology

authors： Schnierle BS,Moss B

更新日期：1992-06-01 00:00:00

abstract：:To elucidate the virulence factors of equine herpesvirus-1 (EHV-1), we compared the genomic architecture of the HH1 strain, a Japanese isolate of EHV-1, with that of its four descendant strains obtained by serial passage in bovine kidney (BK) cells, i.e. BK77, BK161, BK271, and BK343. In preliminary studies high-passa...

journal_title：Virology

authors： Kirisawa R,Ui S,Takahashi A,Kawakami Y,Iwai H

更新日期：1994-05-01 00:00:00

abstract：:Cardioviruses cause diseases in many animals including, in rare cases, humans. Although they share common features with all picornaviruses, cardioviruses have unique properties that distinguish them from other family members, including enteroviruses. One feature shared by all picornaviruses is the covalent attachment ...

journal_title：Virology

authors： Maciejewski S,Ullmer W,Semler BL

更新日期：2018-03-01 00:00:00

abstract：:The Epstein-Barr virus (EBV) nuclear antigen EBNA-1 is essential for viral genome maintenance in vitro and may be the only EBV protein expressed by the majority of latently infected cells in vivo. EBNA-1 may therefore be critical to the evasion of host immunity which allows persistent infection. EBNA-1 includes a poly...

journal_title：Virology

authors： Wrightham MN,Stewart JP,Janjua NJ,Pepper SD,Sample C,Rooney CM,Arrand JR

更新日期：1995-04-20 00:00:00

abstract：:Mice inoculated with replication-competent stocks of the murine acquired immunodeficiency syndrome (MAIDS) virus are severely immunocompromised and proned to the development of T- and B-cell lymphomas. We have studied the development of T-cell lymphomas in C57BL/6 and RF/J mice inoculated with helper-free stocks of th...

journal_title：Virology

authors： Simard C,Huang M,Jolicoeur P

更新日期：1995-01-10 00:00:00

abstract：:About 100 nucleotides of DNA sequence at the 5' noncoding region of the Choristoneura biennis entomopoxvirus spheroidin gene was chemically synthesized and inserted into a vaccinia expression vector, interrupting the vaccinia thymidine kinase gene. When the bacterial beta-galactosidase gene was introduced downstream o...

journal_title：Virology

authors： Pearson A,Richardson C,Yuen L

更新日期：1991-02-01 00:00:00

abstract：:Extensive cell killing and cytopathology were observed within 24 hr after exposure of a clonal cell line of human T-4 lymphocytes (RH9) to culture supernatants containing human immunodeficiency virus (HIV). Ultraviolet-irradiated HIV-containing culture fluids were also capable of killing RH9 cells and of inducing spec...

journal_title：Virology

authors： Rasheed S,Gottlieb AA,Garry RF

更新日期：1986-10-30 00:00:00

abstract：:Pea enation mosaic virus (PEMV) RNA was isolated from virions and then translated in rabbit reticulocyte lysates and in wheat germ extracts. RNA 1 (apparent molecular weight, Mr 1.77 x 106) was shown to code for two major translation products, vp2 (Mr 88,000) and vp4 (Mr 36,000) plus a high-molecular-weight minor prod...

journal_title：Virology

authors： Gabriel CJ,de Zoeten GA

更新日期：1984-12-01 00:00:00

abstract：:In this study we have used an expression system based on Semliki Forest virus (SFV) to study assembly and intracellular localization of certain capsid proteins of rotavirus in neurons and mammalian epithelial cells. The complete genes of vp2 (vp2A) and vp6 (vp6A) of group A rotavirus (SA-11) and gene 5 encoding vp6 (v...

journal_title：Virology

authors： Nilsson M,von Bonsdorff CH,Weclewicz K,Cohen J,Svensson L

更新日期：1998-03-15 00:00:00

abstract：:The large (L) proteins of non-segmented negative stranded (NNS) RNA viruses contain the core RNA dependent RNA polymerase activity for RNA replication and transcription as well as the activities for polyadenylating and capping the mRNA transcripts and for methylating the cap structures. There is currently no structura...

journal_title：Virology

authors： Galloway SE,Richardson PE,Wertz GW

更新日期：2008-12-05 00:00:00

abstract：:While trying to generate a site-directed deletion in the ORF63 latency-associated gene of varicella-zoster virus (VZV) Oka, we constructed a virus with an unexpected rearrangement. The virus has a small deletion in both copies of ORF63 and two copies of a cassette inserted between ORFs 64/65 and 68/69 containing (a) t...

journal_title：Virology

authors： Ambagala AP,Krogmann T,Qin J,Pesnicak L,Cohen JI

更新日期：2010-04-10 00:00:00