Abstract:
:The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading frames (ORFs), 61 were annotated; these annotated ORFs are mainly enzymes involved in phage DNA replication, repair, and nucleotide metabolism. At least five endonucleases that believed to help inducing nicks in T5 genomic DNA, and a DNA ligase gene was found to be split into two separate ORFs. Analysis of T5 early promoters suggests a probable motif AAA{3, 4 T}nTTGCTT{17, 18 n}TATAATA{12, 13 W}{10 R} for strong promoters that may strengthen the step modification of host RNA polymerase, and thus control transcription of phage DNA. The distinct protein domain profile and a mosaic genome structure suggest an origin from the common genetic pool.
journal_name
Virologyjournal_title
Virologyauthors
Wang J,Jiang Y,Vincent M,Sun Y,Yu H,Wang J,Bao Q,Kong H,Hu Sdoi
10.1016/j.virol.2004.10.049subject
Has Abstractpub_date
2005-02-05 00:00:00pages
45-65issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(04)00721-4journal_volume
332pub_type
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