当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Co-selection of West Nile virus nucleotides that confer resistance to an antisense oligomer while maintaining long-distance RNA/RNA base pairings.

Co-selection of West Nile virus nucleotides that confer resistance to an antisense oligomer while maintaining long-distance RNA/RNA base pairings.

Abstract：

:West Nile virus (WNV) genome cyclization is mediated by two pairs of long-distance RNA/RNA interactions: the 5'CS/3'CSI (conserved sequence) and the 5'UAR/3'UAR (upstream AUG region) base pairings. Antisense peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs), designed to interfere with the 5'CS/3'CSI or 5'UAR/3'UAR base pairings, were previously shown to inhibit WNV. In this study, we selected and characterized WNVs resistant to a PPMO targeting the 3'UAR (3'UAR-PPMO). All resistant viruses accumulated one-nucleotide mutations within the 3'UAR, leading to a single-nucleotide mismatch or a weakened base-pairing interaction with the 3'UAR-PPMO. Remarkably, a one-nucleotide mutation within the 5'UAR was correspondingly co-selected; the 5'UAR mutation restored the base pairing with the 3'UAR mutation. Mutagenesis of WNV demonstrated that the single-nucleotide change within the 3'UAR-PPMO-target site conferred the resistance. RNA binding analysis indicated that the single-nucleotide change reduced the ability of 3'UAR-PPMO to block the RNA/RNA interaction required for genome cyclization. The results suggest a mechanism by which WNV develops resistance to 3'UAR-PPMO, through co-selection of the 5'UAR and 3'UAR, to create a mismatch or a weakened base-pairing interaction with the PPMO, while maintaining the 5'UAR/3'UAR base pairings.

journal_name

Virology

journal_title

Virology

authors

Zhang B,Dong H,Stein DA,Shi PY

doi

10.1016/j.virol.2008.08.044

subject

Has Abstract

pub_date

2008-12-05 00:00:00

pages

98-106

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(08)00564-3

journal_volume

382

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • The role of PI3K/Akt in human herpesvirus infection: From the bench to the bedside.

    abstract：:The phosphatidylinositol-3-kinase (PI3K)-Akt signaling pathway regulates several key cellular functions including protein synthesis, cell growth, glucose metabolism, and inflammation. Many viruses have evolved mechanisms to manipulate this signaling pathway to ensure successful virus replication. The human herpesvirus...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2015.02.040

    authors： Liu X,Cohen JI

    更新日期：2015-05-01 00:00:00

  • Characterization of a thymus-tropic HIV-1 isolate from a rapid progressor: role of the envelope.

    abstract：:Loss of T cell homeostasis usually precedes the onset of AIDS. We hypothesized that rapid progressors may be transmitted with HIV-1 that is particularly able to perturb T cell homeostasis. To this end, we have tested two transmitted, syncytium-inducing (SI) viral isolates from a rapid progressor in two thymus models. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.07.019

    authors： Meissner EG,Duus KM,Gao F,Yu XF,Su L

    更新日期：2004-10-10 00:00:00

  • Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization.

    abstract：:RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9497

    authors： Navarro JA,Darós JA,Flores R

    更新日期：1999-01-05 00:00:00

  • Clinical and virological significance of the co-existence of HBsAg and anti-HBs antibodies in hepatitis B chronic carriers.

    abstract：:The co-existence of hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (HBsAb) in serum of hepatitis B virus (HBV)-chronic carriers has been previously associated with HBsAg-amino acid (aa) substitutions. However, the aa pattern of HBV-reverse transcriptase (RT) and the clinical settings associated with this ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.05.012

    authors： Colson P,Borentain P,Motte A,Henry M,Moal V,Botta-Fridlund D,Tamalet C,Gérolami R

    更新日期：2007-10-10 00:00:00

  • Detection of visna virus antigens and RNA in glial cells in foci of demyelination.

    abstract：:Visna is a slow virus infection of sheep in which the characteristic pathological change is demyelination in foci of inflammation. The latter is thought to be the result of an immunopathological process directed against cellular and antigenic targets that have been difficult to define because of restricted viral gene ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90264-8

    authors： Stowring L,Haase AT,Petursson G,Georgsson G,Palsson P,Lutley R,Roos R,Szuchet S

    更新日期：1985-03-01 00:00:00

  • Characterization of the contribution of spliced RNAs of hepatitis B virus to DNA synthesis in transfected cultures of Huh7 and HepG2 cells.

    abstract：:Hepatitis B virus synthesizes multiple spliced RNAs that can be reverse transcribed into viral DNA. We thoroughly characterized the contribution of spliced RNAs to DNA synthesis in transfected cultures of Huh7 and HepG2 cells. We found that up to 50% of DNA within intracellular capsids is derived from five spliced RNA...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.06.021

    authors： Abraham TM,Lewellyn EB,Haines KM,Loeb DD

    更新日期：2008-09-15 00:00:00

  • Mason-Pfizer monkey virus Gag proteins interact with the human sumo conjugating enzyme, hUbc9.

    abstract：:Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00348-9

    authors： Weldon RA Jr,Sarkar P,Brown SM,Weldon SK

    更新日期：2003-09-15 00:00:00

  • Double infection of tobacco protoplasts with brome mosaic virus and cowpea chlorotic mottle virus.

    abstract：:Mixed infections of tobacco protoplasts with Cowpea chlorotic mottle virus (CCMV) and brome mosaic virus (BMV) have been studied. Fluorescent antibody staining showed that capsids of both viruses were made in doubly infected protoplasts but only infectious BMV could be recovered, i.e., BMV dominated in mixed infection...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(80)90050-1

    authors： Watts JW,Dawson JR

    更新日期：1980-09-01 00:00:00

  • Rice tungro bacilliform virus open reading frame 3 encodes a single 37-kDa coat protein.

    abstract：:Rice tungro bacilliform virus (RTBV) is a plant pararetrovirus and a member of the Caulimoviridae family and closely related to viruses in the Badnavirus genus. The coat protein of RTBV is part of the large polyprotein encoded by open reading frame 3 (ORF3). ORF3 of an RTBV isolate from Malaysia was sequenced (accessi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9519

    authors： Marmey P,Bothner B,Jacquot E,de Kochko A,Ong CA,Yot P,Siuzdak G,Beachy RN,Fauquet CM

    更新日期：1999-01-20 00:00:00

  • The ubiquitin-proteasome system is essential for the productive entry of Japanese encephalitis virus.

    abstract：:The host-virus interaction during the cellular entry of Japanese encephalitis virus (JEV) is poorly characterized. The ubiquitin-proteasome system (UPS), the major intracellular proteolytic pathway, mediates diverse cellular processes, including endocytosis and signal transduction, which may be involved in the entry o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.08.013

    authors： Wang S,Liu H,Zu X,Liu Y,Chen L,Zhu X,Zhang L,Zhou Z,Xiao G,Wang W

    更新日期：2016-11-01 00:00:00

  • The complete nucleotide sequence of a New World simian foamy virus.

    abstract：:We determined the complete nucleotide sequence of the New World simian foamy virus (FV) from spider monkey (SFVspm). Starting from a conserved region in the integrase (IN) domain of the pol gene we cloned fragments of the genome up to the 5' end of the long terminal repeat (LTR) into plasmid vectors and elucidated the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.07.018

    authors： Thümer L,Rethwilm A,Holmes EC,Bodem J

    更新日期：2007-12-05 00:00:00

  • Alfalfa mosaic virus temperature-sensitive mutants. V. The nucleotide sequence of TBTS 7 RNA 3 shows limited nucleotide changes and evidence for heterologous recombination.

    abstract：:Nucleotide sequence determination of the coat protein cistron of the alfalfa mosaic virus (AIMV) temperature-sensitive mutant, Tbts 7 (uv) revealed a small number of point mutations of which only one results in the replacement of an amino acid: the asparagine residue at position 126 is replaced by an aspartate residue...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90609-0

    authors： Huisman MJ,Cornelissen BJ,Groenendijk CF,Bol JF,van Vloten-Doting L

    更新日期：1989-08-01 00:00:00

  • The N-terminal part of bacteriophage P2 capsid protein is essential for postassembly maturation of P2 and P4 capsids.

    abstract：:During capsid assembly of bacteriophage P2 and its satellite phage P4, gpN undergoes proteolytic cleavage with the removal of the first 31 amino acids. The truncated protein gpN* is unable to support formation of viable phages in complementation tests. A c-myc antigenic epitope (EQKLISEEDL) exchanged for eleven amino ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0566

    authors： Bjerve K,Lindqvist BH

    更新日期：1996-10-15 00:00:00

  • Inhibition of HIV-1 replication by cyclosporine A or related compounds correlates with the ability to disrupt the Gag-cyclophilin A interaction.

    abstract：:The HIV-1 Gag polyprotein specifically incorporates the cellular peptidylprolyl isomerase cyclophilin A into virions. HIV-1 replication is inhibited by cyclosporine A, an immunosuppressive drug which binds with high affinity to cyclophilin A and precludes interaction with the Gag polyprotein. Using a panel of four dru...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0421

    authors： Franke EK,Luban J

    更新日期：1996-08-01 00:00:00

  • Cellular basis of potato spindle tuber viroid systemic movement.

    abstract：:Viroids are small, nontranslatable pathogenic RNAs that replicate autonomously and traffic systemically in their host plants. We have used in situ hybridization to analyze the trafficking pattern of Potato spindle tuber viroid (PSTVd) in tomato and Nicotiana benthamiana. When PSTVd was inoculated onto the stem of a pl...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0724

    authors： Zhu Y,Green L,Woo YM,Owens R,Ding B

    更新日期：2001-01-05 00:00:00

  • Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide.

    abstract：:Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90199-2

    authors： Pugh JC,Summers JW

    更新日期：1989-10-01 00:00:00

  • Development of reassortant viruses between pathogenic hantavirus strains.

    abstract：:Segment reassortment of negative strand viruses is an important mechanism for the development of new virus strains with altered pathogenicity. This study reports on in vitro generation of reassortants between Andes (ANDV) and Sin Nombre (SNV) viruses. Although they both cause hantavirus pulmonary syndrome (HPS), ANDV ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.07.012

    authors： Rizvanov AA,Khaiboullina SF,St Jeor S

    更新日期：2004-10-01 00:00:00

  • Identification of the 5' end of the rubella virus subgenomic RNA.

    abstract：:The 5' end of the subgenomic RNA of rubella virus was determined by primer extension. The Maxam-Gilbert sequence ladder of the primer extension product contained a determinable sequence which was colinear with the complement of the sequence of the genomic RNA through nucleotide 3325 from the 3' end of the genomic RNA ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90422-4

    authors： Frey TK,Marr LD,Sanchez A,Simmons RB

    更新日期：1989-01-01 00:00:00

  • Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells.

    abstract：:Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.05.023

    authors： Soboll Hussey G,Ashton LV,Quintana AM,Van de Walle GR,Osterrieder N,Lunn DP

    更新日期：2014-09-01 00:00:00

  • HIV is inactivated after transepithelial migration via adult oral epithelial cells but not fetal epithelial cells.

    abstract：:Oral transmission of human immunodeficiency virus (HIV) in adult populations is rare. However, HIV spread across fetal/neonatal oropharyngeal epithelia could be important in mother-to-child transmission. Analysis of HIV transmission across polarized adult and fetal oral epithelial cells revealed that HIV transmigrates...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.10.004

    authors： Tugizov SM,Herrera R,Veluppillai P,Greenspan D,Soros V,Greene WC,Levy JA,Palefsky JM

    更新日期：2011-01-20 00:00:00

  • Characterization of proteinase cleavage sites in the N-terminal region of the RNA1-encoded polyprotein from Arabis mosaic virus (subgroup A nepovirus).

    abstract：:Arabis mosaic virus is a subgroup A nepovirus. The RNA1-encoded polyprotein (P1) contains the domains for the NTP-binding protein (NTB), VPg, proteinase (Pro) and polymerase at its C-terminus. Putative cleavage sites delineating these domains have been proposed. However, the number and location of cleavage sites upstr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.01.046

    authors： Wetzel T,Chisholm J,Bassler A,Sanfaçon H

    更新日期：2008-05-25 00:00:00

  • Analysis of the site occupancy constraints of primary amino acid sequences in the motif directing palmitylation of the vaccinia virus 37-kDa envelope protein.

    abstract：:Vaccinia virus (VV) encodes a 37-kDa envelope protein (p37) that is palmitylated on cysteine residues 185186 of the 372-amino acid protein. We have previously reported on a loosely conserved consensus motif. Further analysis has identified a conserved consensus sequence, Hydro*AAC(C)A (Hydro* represents a hydrophobic ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9543

    authors： Hansen SG,Grosenbach DW,Hruby DE

    更新日期：1999-02-01 00:00:00

  • Homologous and heterologous complementation of HBV and WHV capsid and polymerase functions in RNA encapsidation.

    abstract：:Successful encapsidation of hepadnaviral pregenomic RNA requires the orchestrated interaction of the viral capsid and polymerase proteins with each other and with the RNA packaging substrate. The early steps of this process involve binding of the polymerase to the encapsidation signal, epsilon, and are already underst...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0260

    authors： Ziermann R,Ganem D

    更新日期：1996-05-15 00:00:00

  • In vitro fidelity of the prototype primate foamy virus (PFV) RT compared to HIV-1 RT.

    abstract：:We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.05.034

    authors： Boyer PL,Stenbak CR,Hoberman D,Linial ML,Hughes SH

    更新日期：2007-10-25 00:00:00

  • H4N8 subtype avian influenza virus isolated from shorebirds contains a unique PB1 gene and causes severe respiratory disease in mice.

    abstract：:H4N8 subtype avian influenza viruses were isolated from shorebirds in eastern Hokkaido. All the isolates shared >99.7% nucleotide homology, and all the viral genes except for PB1 were highly related to those of A/red-necked stint/Australia/1/04. Thus, the isolates were regarded as PB1 reassortants. The most similar PB...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.11.019

    authors： Bui VN,Ogawa H,Xininigen,Karibe K,Matsuo K,Awad SS,Minoungou GL,Yoden S,Haneda H,Ngo LH,Tamaki S,Yamamoto Y,Nakamura K,Saito K,Watanabe Y,Runstadler J,Huettmann F,Happ GM,Imai K

    更新日期：2012-02-05 00:00:00

  • The UL37 protein of herpes simplex virus type 1 is associated with the tegument of purified virions.

    abstract：:The herpes simplex virus type 1 (HSV-1) UL37 open reading frame encodes a 120-kDa late (gamma 1) phosphoprotein in infected cells. Analysis of isolated mature HSV virions and light particles revealed that the UL37 protein is a component of the virion. Detergent solubilization and protease digestion experiments suggest...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1028

    authors： Schmitz JB,Albright AG,Kinchington PR,Jenkins FJ

    更新日期：1995-02-01 00:00:00

  • Combination of Adt-O1Manisa and Ad5-boIFNλ3 induces early protective immunity against foot-and-mouth disease in cattle.

    abstract：:Foot-and-mouth-disease (FMD) remains the most infectious livestock disease worldwide. Although commercially available inactivated or adenovirus-vectored-vaccines (Ad5-FMD) are effective, they require 5-7 days to induce protection. Therefore, new control strategies that stimulate rapid immune responses are needed. Expr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.09.027

    authors： Diaz-San Segundo F,Montiel NA,Sturza DF,Perez-Martin E,Hickman D,Ramirez-Medina E,Grubman MJ,de Los Santos T

    更新日期：2016-12-01 00:00:00

  • Immune response in rabbit ovaries following infection of a recombinant myxoma virus expressing rabbit zona pellucida protein B.

    abstract：:In this study, we investigated the autoimmune response in rabbit ovaries following infection with a recombinant myxoma virus expressing rabbit zona pellucida protein B (MV-ZPB). A specific IgG antibody response to ZPB was elicited in the serum of infected rabbits and the antibody strongly bound to the zona pellucida o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2003.10.021

    authors： Gu W,Holland M,Janssens P,Seamark R,Kerr P

    更新日期：2004-01-20 00:00:00

  • Drosophila as a genetic model for studying pathogenic human viruses.

    abstract：:Viruses are infectious particles whose viability is dependent on the cells of living organisms, such as bacteria, plants, and animals. It is of great interest to discover how viruses function inside host cells in order to develop therapies to treat virally infected organisms. The fruit fly Drosophila melanogaster is a...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2011.11.016

    authors： Hughes TT,Allen AL,Bardin JE,Christian MN,Daimon K,Dozier KD,Hansen CL,Holcomb LM,Ahlander J

    更新日期：2012-02-05 00:00:00

  • Translational studies with turnip yellow mosaic virus RNAs isolated from major and minor virus particles.

    abstract：:The major and minor nucleoprotein components in turnip yellow mosaic virus (TYMV) preparations have been fractionated by four cycles of CsCl gradient equilibrium centrifugations. RNAs isolated from the various fractions were translated in a rabbit reticulocyte cell-free system. Three classes of RNAs were studied: geno...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(79)90170-3

    authors： Mellema JR,Benicourt C,Haenni AL,Noort A,Pleij CW,Bosch L

    更新日期：1979-07-15 00:00:00