Translational studies with turnip yellow mosaic virus RNAs isolated from major and minor virus particles.

abstract：:Infections of nonpermissive ( sup0 ) Escherichia coli by T1 phage with amber mutations in either gene 3.5 or gene 4 exhibit a variety of defective phenotypes, including premature arrest of T1 DNA synthesis, failure to make concatemeric DNA, formation of an abnormal DNA replication intermediate, failure to package phag...

journal_title：Virology

authors： Pugh JC,Ritchie DA

更新日期：1984-05-01 00:00:00

abstract：:Recent findings suggested that the SUMO-interacting motifs (SIMs) present in the human TRIM5α (TRIM5α(hu)) protein play an important role in the ability of TRIM5α(hu) to restrict N-MLV. Here we explored the role of SIMs in the ability of rhesus TRIM5α (TRIM5α(rh)) to restrict HIV-1, and found that TRIM5α(rh) SIM mutan...

journal_title：Virology

authors： Brandariz-Nuñez A,Roa A,Valle-Casuso JC,Biris N,Ivanov D,Diaz-Griffero F

更新日期：2013-01-20 00:00:00

abstract：:MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...

journal_title：Virology

authors： Yuen PH,Soong MM,Kissil MS,Wong PK

更新日期：1984-01-30 00:00:00

abstract：:The 20,349-bp sequence of the human cytomegalovirus (HCMV) HindIII F fragment has revealed eight open reading frames with homology to herpes simplex virus (HSV) and/or Epstein-Barr virus (EBV). With respect to EBV, these homologous genes can be divided into two blocks: one block contains three genes, including the DNA...

journal_title：Virology

authors： Kouzarides T,Bankier AT,Satchwell SC,Weston K,Tomlinson P,Barrell BG

更新日期：1987-04-01 00:00:00

abstract：:The interaction between the viral envelope protein gp120 and the cellular surface antigen CD4 is a key event in HIV-1 infection. Reciprocal high affinity binding sites have been located in the first domain of CD4 and in the carboxy-terminal region of gp120, respectively. Upon infection, the membranes of the target cel...

journal_title：Virology

authors： Autiero M,Abrescia P,Dettin M,Di Bello C,Guardiola J

更新日期：1991-12-01 00:00:00

abstract：:We have used field inversion gel electrophoresis to survey EBV strains for the size of the major internal repeat, IR-1, and estimate the number of 3.1-kb repeat units present. The B95-8 strain of EBV was estimated to contain 8.6 repeats. The repeat number varies considerably among naturally occurring isolates around a...

journal_title：Virology

authors： Allan GJ,Rowe DT

更新日期：1989-12-01 00:00:00

abstract：:Expression of Kaposi's sarcoma herpesvirus vFLIP, a potent activator of NFkB signaling, promotes latency. Inhibition of NFkB signaling promotes lytic reactivation. We previously reported that lytic inducer, RTA, inhibits vFLIP induced NFkB signaling by inducing the degradation of vFLIP via the proteasome. Here we repo...

journal_title：Virology

authors： Chmura JC,Herold K,Ruffin A,Atuobi T,Fabiyi Y,Mitchell AE,Choi YB,Ehrlich ES

更新日期：2017-01-15 00:00:00

abstract：:The E7 oncoprotein of high risk human papillomavirus type 16 (HPV16) binds and inactivates the retinoblastoma (RB) family of proteins. Our previous studies suggested that HPV16 E7 enters the nucleus via a novel Ran-dependent pathway independent of the nuclear import receptors (Angeline, M., Merle, E., and Moroianu, J....

journal_title：Virology

authors： Knapp AA,McManus PM,Bockstall K,Moroianu J

更新日期：2009-01-05 00:00:00

abstract：:Vif is essential for HIV-1 replication in T cells and macrophages. Vif recruits a host ubiquitin ligase complex to promote proteasomal degradation of the APOBEC3 restriction factors by poly-ubiquitination. The cellular transcription cofactor CBFβ is required for Vif function by stabilizing the Vif protein and promotin...

journal_title：Virology

authors： Matsui Y,Shindo K,Nagata K,Io K,Tada K,Iwai F,Kobayashi M,Kadowaki N,Harris RS,Takaori-Kondo A

更新日期：2014-01-20 00:00:00

abstract：:Adenoviruses with deletion of E1b have been used in clinical trials to treat cancers that are resistant to conventional therapies. The efficacy of viral replication within cancer cells determines the results of oncolytic therapy, which remains poorly understood and requires further improvement. In this report, we show...

journal_title：Virology

authors： Rodriguez-Rocha H,Gomez-Gutierrez JG,Garcia-Garcia A,Rao XM,Chen L,McMasters KM,Zhou HS

更新日期：2011-07-20 00:00:00

abstract：:This report presents a procedure for evaluating the relative affinities of simple sialoside receptor determinants for their interaction with the receptor binding pocket of the influenza virus hemagglutinin. The virus examined was A/Memphis/102/72, previously shown to exhibit preferential binding specificity for enzyma...

journal_title：Virology

authors： Pritchett TJ,Brossmer R,Rose U,Paulson JC

更新日期：1987-10-01 00:00:00

abstract：:We constructed a sensitive and quantitative assay system to examine human T-cell leukemia virus type I (HTLV-I) envelope (env) glycoprotein-mediated cell fusion in which T7 RNA polymerase in donor cells coexpressing env glycoproteins activates a reporter gene in recipient cells upon cell fusion. An efficient expressio...

journal_title：Virology

authors： Okuma K,Nakamura M,Nakano S,Niho Y,Matsuura Y

更新日期：1999-02-15 00:00:00

abstract：:The effect of murine cytomegalovirus (MCMV) infection on interleukin 1 (IL-1) secretion was assessed using the macrophage cell lines P388D1 and J774A.1. The former proved to be nonpermissive for MCMV in that infectious virus and viral immediate early protein (pp89) were not expressed in these cells. MCMV infection of ...

journal_title：Virology

authors： Lewis MA,Slater JS,Leverone LI,Campbell AE

更新日期：1990-10-01 00:00:00

abstract：:The eukaryotic translation initiation factor eIF4E, the cap-binding protein, seems to play an essential role in the establishment of the host shut-off after viral infection. Infection with adenovirus and influenza virus caused dephosphorylation of eIF4E and an involvement of a viral protein was suggested. In this repo...

journal_title：Virology

authors： Kleijn M,Vrins CL,Voorma HO,Thomas AA

更新日期：1996-03-15 00:00:00

abstract：:Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...

journal_title：Virology

authors： Julander JG,Siddharthan V,Blatt LM,Schafer K,Sidwell RW,Morrey JD

更新日期：2007-04-10 00:00:00

abstract：:Mutants of adenovirus type 5 (Ad5) that lack early region 4 (E4) are defective in the expression of viral late genes. E4 mutants exhibit dramatically reduced levels of both cytoplasmic and nuclear viral late RNAs compared to wild-type virus, due principally to reduced stability of unprocessed viral late RNA in the nuc...

journal_title：Virology

authors： Sandler AB,Ketner G

更新日期：1991-03-01 00:00:00

abstract：:LMP2A deletion and point mutants, with mutations in phosphotyrosine-containing protein-protein interaction motifs, were transiently expressed in 293 cells and their phosphorylation was examined in immune complex kinase assays as well as in vivo. In vitro LMP2A phosphorylation depended on tyrosine 112. In vivo, mutatio...

journal_title：Virology

authors： Scholle F,Longnecker R,Raab-Traub N

更新日期：2001-12-20 00:00:00

abstract：:KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...

journal_title：Virology

authors： DeMaster LK,Rose TM

更新日期：2014-01-05 00:00:00

abstract：:Trans-acting regulatory components of Autographa californica nuclear polyhedrosis virus (AcMNPV) were studied in a transient assay system for their ability to activate the p143 gene promoter. A region including 502 nt upstream of the AUG of the p143 gene was linked to the bacterial chloramphenicol acetyltransferase (C...

journal_title：Virology

authors： Lu A,Carstens EB

更新日期：1993-08-01 00:00:00

abstract：:Studies using transformed human cell lines suggest that most SIV strains use CCR5 as co-receptor. Our analysis of primary rhesus macaque CD4(+) T-cell clones revealed marked differences in susceptibility to SIV(mac)239 infection. We investigated whether different levels of CCR5 expression account for clonal difference...

journal_title：Virology

authors： Minang JT,Trivett MT,Barsov EV,Del Prete GQ,Trubey CM,Thomas JA,Gorelick RJ,Piatak M Jr,Ott DE,Ohlen C

更新日期：2011-01-05 00:00:00

abstract：:CD8+ T lymphocytes are critical for the control of gammaherpesvirus latency. To determine how memory CD8+ T cells generated during latency differ from those primed during acute or chronic viral infection, we adoptively transferred naive P14 CD8+ T cells into uninfected recipients, and examined surface proteins, cytoki...

journal_title：Virology

authors： Liu M,Barton ES,Jennings RN,Oldenburg DG,Whirry JM,White DW,Grayson JM

更新日期：2017-09-01 00:00:00

abstract：:The influenza A virus nucleoprotein previously expressed in Escherichia coli after fusion to 32 heterologous amino acids has now been purified and tested for its ability to form complexes with RNA in vitro. By using a simple filter binding assay, we show that ribonucleoprotein (RNP) complexes form readily with single-...

journal_title：Virology

authors： Kingsbury DW,Jones IM,Murti KG

更新日期：1987-02-01 00:00:00

abstract：:Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional...

journal_title：Virology

authors： Larson ET,Eilers BJ,Reiter D,Ortmann AC,Young MJ,Lawrence CM

更新日期：2007-07-05 00:00:00

abstract：:To investigate differential evolutionary rates and selective forces of WNV in hosts and vectors, we measured the genetic diversity that arose during alternating passage in mosquitoes and birds. Within-host genetic diversity was monitored in each of three experimentally passed replicates, and the complete genome sequen...

journal_title：Virology

authors： Jerzak GV,Brown I,Shi PY,Kramer LD,Ebel GD

更新日期：2008-05-10 00:00:00

abstract：:Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...

journal_title：Virology

authors： Leinbach SS,Casto JF,Pickett TK

更新日期：1984-09-01 00:00:00

abstract：:The open reading frame of the large subunit (R1) of herpes simplex virus type 1 (HSV-1) ribonucleotide reductase has been positioned downstream of the phage T7 gene 10 promoter in the expression vector, pET. Transformation of this recombinant plasmid into Escherichia coli BL21 DE3 cells containing the T7 RNA polymeras...

journal_title：Virology

authors： Furlong J,Conner J,McLauchlan J,Lankinen H,Galt C,Marsden HS,Clements JB

更新日期：1991-06-01 00:00:00

abstract：:A series of clones has been derived from an interferon-resistant murine cell line, Ltk- aprt-, and their antiviral properties have been characterized. In the parental Ltk- aprt- line interferon is unable to establish antiviral properties or to increase the levels of 2,5-oligo(A) synthetase, the 2,5-oligo(A)-activated ...

journal_title：Virology

authors： Lewis JA

更新日期：1988-01-01 00:00:00

abstract：:West Nile virus (WNV) genome cyclization is mediated by two pairs of long-distance RNA/RNA interactions: the 5'CS/3'CSI (conserved sequence) and the 5'UAR/3'UAR (upstream AUG region) base pairings. Antisense peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs), designed to interfere with the 5'CS/3'CSI o...

journal_title：Virology

authors： Zhang B,Dong H,Stein DA,Shi PY

更新日期：2008-12-05 00:00:00

abstract：:The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for...

journal_title：Virology

authors： Daksis JI,Preston CM

更新日期：1992-07-01 00:00:00

abstract：:The mechanism by which the large-size poxviruses enter animal cells is not known. In this investigation we show that acid pH treatment of wild-type vaccinia virus-infected cells triggers strong fusion of cells in culture, with an optimum at pH 4.8. We have identified the virus-induced fusion protein as a 14-kDa envelo...

journal_title：Virology

authors： Gong SC,Lai CF,Esteban M

更新日期：1990-09-01 00:00:00