Induction of an antiviral state by interferon in the absence of elevated levels of 2,5-oligo(A) synthetase and eIF-2 kinase.

abstract：:Bovine herpesvirus 5 (BHV-5) is a neurovirulent alpha-herpesvirus that causes fatal encephalitis in calves. We previously demonstrated that deletion of a glycine-rich epitope in the gE ectodomain dramatically reduced BHV-5 neurovirulence. To investigate the role of gE cytoplasmic tail sequences in the neuropathogenesi...

journal_title：Virology

authors： Al-Mubarak A,Simon J,Coats C,Okemba JD,Burton MD,Chowdhury SI

更新日期：2007-09-01 00:00:00

abstract：:The interferon sensitivity of the expression of an influenza-virus hemagglutinin (HA) gene cloned into the thymidine kinase (TK) gene of vaccinia virus was studied in chick embryo fibroblasts (CEF) and Madin-Darby bovine kidney (MDBK) cells. In CEF, the expression of the HA gene is inhibited by pretreatment of cells w...

journal_title：Virology

authors： Degen HJ,Blum D,Grün J,Jungwirth C

更新日期：1992-05-01 00:00:00

abstract：:Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B...

journal_title：Virology

authors： Sawada Y,Föhring B,Shenk TE,Raska K Jr

更新日期：1985-12-01 00:00:00

abstract：:Attenuated mutants of Venezuelan equine encephalitis virus (VEE) were isolated by selection for rapid penetration of cultured cells (R. E. Johnston and J. F. Smith, 1988, Virology 162, 437-443). Sequence analysis of these mutants identified candidate attenuating mutations at four loci in the VEE E2 glycoprotein gene: ...

journal_title：Virology

authors： Davis NL,Powell N,Greenwald GF,Willis LV,Johnson BJ,Smith JF,Johnston RE

更新日期：1991-07-01 00:00:00

abstract：:African Swine fever virus (ASFV) genome is a large (170-190 kb) double-stranded DNA molecule with structural features similar to those of Poxviruses. Prominent among those features are the presence of a hairpin loop structure at the end of the DNA molecule and terminal-inverted repeats (TIR). The TIRs have been previo...

journal_title：Virology

authors： de la Vega I,González A,Blasco R,Calvo V,Viñuela E

更新日期：1994-05-15 00:00:00

abstract：:Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants....

journal_title：Virology

authors： MacFarlane SA,Gilmer D,Davies JW

更新日期：1992-04-01 00:00:00

abstract：:Certain temperature-sensitive (ts) mutants of murine leukemia virus (MuLV) were observed to be defective in virus assembly. These mutants also accumulated intracellular core protein precursor, Pr65gag, at 39 degrees, the nonpermissive temperature. At 39 degrees, virions released from cells infected with the various ts...

journal_title：Virology

authors： Edbauer CA,Naso RB

更新日期：1984-04-30 00:00:00

abstract：:The relationship between the cell cycle and the presence of the nuclear T antigen in simian virus 40-transformed rat cells independently derived from FR 3T3 cells has been investigated using a fluorescence-activated cell sorter and a combination of stainings for cellular DNA (4',6-diamidine-2-phenylindole dihydrochlor...

journal_title：Virology

authors： Imbert J,Lawrence JJ,Birg F

更新日期：1983-04-30 00:00:00

abstract：:The DNAs from the granulosis viruses of Plodia interpunctella and Pieris rapae were clearly distinguishable based on restriction endonuclease fragment patterns. By sizing single- and double-digestion products approximate molecular weights of 72 x 10(2) and 75 x 10(6), respectively, were estimated for the viral genomes...

journal_title：Virology

authors： Tweeten KA,Bulla LA Jr,Consigli RA

更新日期：1980-07-30 00:00:00

abstract：:The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, induces an acutely lethal disease in experimentally inoculated pigtailed macaques, that is characterized by severe enteropathy and extensive immune activation, particularly within gut-associated lymphoid tissue (GALT). Experiments were conducted to deter...

journal_title：Virology

authors： Gummuluru S,Novembre FJ,Lewis M,Gelbard HA,Dewhurst S

更新日期：1996-11-01 00:00:00

abstract：:The hepatitis B virus core open reading frame with and without the precore domain was expressed in insect cells using a baculovirus expression system. Precore antigen was not properly processed in insect cells and was present in highly insoluble cytoplasmic aggregates. Core antigen without the precore domain formed co...

journal_title：Virology

authors： Lanford RE,Notvall L

更新日期：1990-05-01 00:00:00

abstract：:We examined the native plant host Solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (TYLCD) emerging in southern Spain. Presence of isolates of all the species and strains found associated with TYLCD in this area was demonstrated. Mixed infections were com...

journal_title：Virology

authors： García-Andrés S,Monci F,Navas-Castillo J,Moriones E

更新日期：2006-07-05 00:00:00

abstract：:HIV-1 entry into cells requires the interaction of both HIV-1 envelope proteins and membrane lipids. We investigated the mechanism of neutralization of HIV-1 infection of primary monocyte-derived macrophages (MDM) by a murine monoclonal antibody (mAb) WR321. WR321 specifically binds phosphatidylinositol-4-phosphate an...

journal_title：Virology

authors： Jobe O,Peachman KK,Matyas GR,Asher LV,Alving CR,Rao M

更新日期：2012-09-01 00:00:00

abstract：:NF-kappaB comprises a family of transcription factors that regulate key immune processes. In this study, the effects of orthopoxvirus infection upon the activation of NF-kappaB were examined. During the early phase of infection, cowpox virus can inhibit the induction of NF-kappaB-regulated gene expression by interferi...

journal_title：Virology

authors： Oie KL,Pickup DJ

更新日期：2001-09-15 00:00:00

abstract：:The nucleotide sequence of the gene immediately following the nucleocapsid protein gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, is presented. Serological reactions of SYNV proteins with antibodies elicited by a fusion protein constructed from the sequenced gene indicate that this gene encodes an SYNV ...

journal_title：Virology

authors： Heaton LA,Zuidema D,Jackson AO

更新日期：1987-11-01 00:00:00

abstract：:In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian clade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eu...

journal_title：Virology

authors： Bertran K,Swayne DE,Pantin-Jackwood MJ,Kapczynski DR,Spackman E,Suarez DL

更新日期：2016-07-01 00:00:00

abstract：:A 140,000-dalton disulfide-linked glycoprotein (gp140) specified by varicella-zoster virus (VZV) in infected cultured cells was identified and precipitated by two murine monoclonal antibodies (VZ-151 and VZ-158). When analyzed under reducing conditions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gp14...

journal_title：Virology

authors： Grose C,Edwards DP,Weigle KA,Friedrichs WE,McGuire WL

更新日期：1984-01-15 00:00:00

abstract：:HeLa cell extracts containing wild-type copies of the minute virus of mice NS-1 polypeptide produced from a recombinant vaccinia virus vector could support the excision and replication of viral 5' telomeres from cloned concatemer junction fragments. Resolution did not occur if wild-type NS-1 was omitted from the extra...

journal_title：Virology

authors： Cotmore SF,Nuesch JP,Tattersall P

更新日期：1992-09-01 00:00:00

abstract：:Following infection with murine cytomegalovirus (MCMV), the termini of the linear double-stranded DNA genome fuse to form circular or concatemeric forms which serve as replicative intermediates. To investigate the mechanisms involved in the generation and cleavage of the intracellular concatenates, we have used restri...

journal_title：Virology

authors： Marks JR,Spector DH

更新日期：1988-01-01 00:00:00

abstract：:Human papillomaviruses (HPVs) infect epithelial cells and are associated with genital carcinoma. Most epithelial cell lines express cell-surface glycosaminoglycans (GAGs) usually found attached to the protein core of proteoglycans. Our aim was to study how GAGs influenced HPV entry. Using a human keratinocyte cell lin...

journal_title：Virology

authors： Drobni P,Mistry N,McMillan N,Evander M

更新日期：2003-05-25 00:00:00

abstract：:The presence of a genome-linked protein (VPg) at the RNA 5'-end of the genome is a characteristic of different groups of animal and plant positive-sense single-stranded RNA viruses. These viruses express their structural and functional proteins from polyproteins that are sequentially processed by at least one viral pr...

journal_title：Virology

authors： Margis R,Viry M,Pinck M,Bardonnet N,Pinck L

更新日期：1994-04-01 00:00:00

abstract：:Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in...

journal_title：Virology

authors： Watanabe R,Leser GP,Lamb RA

更新日期：2011-08-15 00:00:00

abstract：:The transcripts of the DNA of Abutilon mosaic virus, a geminivirus with a bipartite genome (DNA A and DNA B), were characterized by Northern blot hybridization, S1 nuclease assay, primer extension analysis, and sequencing of the 3' termini of cDNA clones. It was shown that transcription is bidirectional and that the t...

journal_title：Virology

authors： Frischmuth S,Frischmuth T,Jeske H

更新日期：1991-12-01 00:00:00

abstract：:A distinct host cellular protein binding element was mapped within a 38-nucleotide (nt) sequence 166-129 nucleotides upstream of the 3' end of the MHV-JHM genome using a RNase T1 protection/gel mobility shift electrophoresis assay. The resultant RNA-protein complex contains six host cellular proteins, one protein of 1...

journal_title：Virology

authors： Liu Q,Yu W,Leibowitz JL

更新日期：1997-05-26 00:00:00

abstract：:Lysolecithin permeabilization of vaccinia virus-infected cells was employed to prepare extracts that support faithful transcription initiation in vitro on plasmids possessing early, intermediate, and late viral gene promoters. Conditions which optimize transcription from each promoter were defined. The in vitro system...

journal_title：Virology

authors： Condit RC,Lewis JI,Quinn M,Christen LM,Niles EG

更新日期：1996-04-01 00:00:00

abstract：:We have constructed chimeric papillomavirus-like particles (CVLPs) by replacing the 34-carboxy-terminal amino acids of the HPV 16 L1 protein with various parts of the HPV 16 E7 protein. Chimeric proteins were expressed by recombinant baculoviruses and analyzed by electron microscopy for their ability to assemble into ...

journal_title：Virology

authors： Müller M,Zhou J,Reed TD,Rittmüller C,Burger A,Gabelsberger J,Braspenning J,Gissmann L

更新日期：1997-07-21 00:00:00

abstract：:KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...

journal_title：Virology

authors： DeMaster LK,Rose TM

更新日期：2014-01-05 00:00:00

abstract：:HCV culture in vitro results in massive cell death, which suggests the presence of HCV-induced cytopathic effects. Therefore, we investigated its mechanisms and viral nucleotide sequences involved in this effect using HCV-JFH1 cell culture and a newly developed HCV plaque assay technique. The plaque assay developed cy...

journal_title：Virology

authors： Sekine-Osajima Y,Sakamoto N,Mishima K,Nakagawa M,Itsui Y,Tasaka M,Nishimura-Sakurai Y,Chen CH,Kanai T,Tsuchiya K,Wakita T,Enomoto N,Watanabe M

更新日期：2008-02-05 00:00:00

abstract：:TMV capsid protein reacts with and encapsidates many of the chloroplast DNA transcripts both in vivo and in vitro to form pseudovirions. We report on the encapsidation initiation reaction with one of the major RNA species found in in vivo formed pseudovirions, the mRNA for the chloroplast-encoded large subunit of ribu...

journal_title：Virology

authors： Atreya CD,Siegel A

更新日期：1989-02-01 00:00:00

abstract：:We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as ass...

journal_title：Virology

authors： Saha S,Yoshida S,Ohba K,Matsui K,Matsuda T,Takeshita F,Umeda K,Tamura Y,Okuda K,Klinman D,Xin KQ,Okuda K

更新日期：2006-10-10 00:00:00