Abstract:
:We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as assessed by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICCS). These mice also showed maximal resistance following challenge with the A/PR/8/34 (H1N1) and A/Udron/72 (H3N2) strains of influenza virus. The susceptibility of immunized mice to virus infection was significantly increased following depletion of either CD4+ or CD8+ T cells. These results indicate that a plasmid DNA vaccine encoding pVP22 plus NP induces a high level of cross-protective immunity against influenza virus subtypes.
journal_name
Virologyjournal_title
Virologyauthors
Saha S,Yoshida S,Ohba K,Matsui K,Matsuda T,Takeshita F,Umeda K,Tamura Y,Okuda K,Klinman D,Xin KQ,Okuda Kdoi
10.1016/j.virol.2006.04.015subject
Has Abstractpub_date
2006-10-10 00:00:00pages
48-57issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00263-7journal_volume
354pub_type
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