Abstract:
:The influenza PA-X protein is translated from the PA open reading frame from frameshifting and suppresses cellular gene expression due to its ribonuclease activity. We further defined the functional roles of PA-X by comparing PA-X proteins from two related viruses - equine influenza (EIV) and canine influenza (CIV) H3N8 - that differ in a C-terminal truncation and internal mutations. In vitro reporter gene assays revealed that both proteins were able to suppress gene expression. Interestingly, EIV PA-X demonstrated ~50% greater activity compared to CIV PA-X, and we identified the mutations that caused this difference. We used RNA-seq to evaluate the effects of PA-X on host gene expression after transfection into cultured cells. There were no significant differences in this property between EIV and CIV PA-X proteins, but expression of either resulted in the up-regulation of genes when compared to controls, most notably immunity-related proteins, trafficking proteins, and transcription factors.
journal_name
Virologyjournal_title
Virologyauthors
Feng KH,Sun M,Iketani S,Holmes EC,Parrish CRdoi
10.1016/j.virol.2016.06.001subject
Has Abstractpub_date
2016-09-01 00:00:00pages
138-146eissn
0042-6822issn
1096-0341pii
S0042-6822(16)30135-0journal_volume
496pub_type
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