当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Homotypic interaction and multimerization of hepatitis C virus core protein.

Homotypic interaction and multimerization of hepatitis C virus core protein.

Abstract：

:Hepatitis C virus (HCV) core protein constitutes a viral nucleocapsid and may possess multiple functions. In this study, we demonstrated the homotypic interaction and multimerization of HCV core protein in vitro and in vivo. By using a yeast two-hybrid system, we showed that the amino-terminal hydrophilic portion (amino acids 1-115) of the core protein could interact with itself. Deletion analysis mapped the interacting domain within amino acid residues 36-91. The homotypic interaction of the core protein was also confirmed by in vitro protein-protein blotting assay using the recombinant HCV core proteins and by its binding to the glutathione S-transferase core fusion protein. The biological significance of the core protein self-interactions was demonstrated by the detection of multimeric forms of the core protein in mammalian cells. The domain responsible for multimerization was determined to be within the amino-terminal hydrophilic region (amino acids 1-115). Both the membrane-bound and the free core proteins exist in dimeric and multimeric forms, suggesting that multimerization of the HCV core protein occurred at an early stage of viral assembly and that the multimer forms may be involved in multiple functions of the core protein.

journal_name

Virology

journal_title

Virology

authors

Matsumoto M,Hwang SB,Jeng KS,Zhu N,Lai MM

doi

10.1006/viro.1996.0164

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

43-51

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(96)90164-6

journal_volume

218

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • The spiroplasma virus 4 replicative form cloned in Escherichia coli transfects spiroplasmas.

    abstract：:The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90060-7

    authors： Pascarel-Devilder MC,Renaudin J,Bove JM

    更新日期：1986-06-01 00:00:00

  • Pandemic H1N1 influenza A directly induces a robust and acute inflammatory gene signature in primary human bronchial epithelial cells downstream of membrane fusion.

    abstract：:Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.09.022

    authors： Paquette SG,Banner D,Chi le TB,Leόn AJ,Xu L,Ran L,Huang SS,Farooqui A,Kelvin DJ,Kelvin AA

    更新日期：2014-01-05 00:00:00

  • Identification and characterization of an equine herpesvirus 1 late gene encoding a potential zinc finger.

    abstract：:In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90525-t

    authors： Holden VR,Yalamanchili RR,Harty RN,O'Callaghan DJ

    更新日期：1992-06-01 00:00:00

  • Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis.

    abstract：:The potential role(s) of cytokines in the reduction of infectious virus and persistent viral infection in the central nervous system was examined by determining the kinetics of cytokine mRNA expression following infection with the neurotropic JHM strain of mouse hepatitis virus. Mice were infected with an antibody esc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8613

    authors： Parra B,Hinton DR,Lin MT,Cua DJ,Stohlman SA

    更新日期：1997-07-07 00:00:00

  • Production of cucumber mosaic virus RNA5 and its role in recombination.

    abstract：:Cucumber Mosaic Virus (CMV) is a plant infecting tripartite positive-strand RNA virus. In addition to three genomic and two known subgenomic RNAs, CMV strains of subgroup II (e.g. Q-CMV), but not subgroup I (e.g. Fny-CMV), produce and package a redundant RNA5 encompassing the 3' 304-307 nucleotides of RNAs 2 and 3. Th...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.10.023

    authors： de Wispelaere M,Rao AL

    更新日期：2009-02-05 00:00:00

  • Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide.

    abstract：:Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90199-2

    authors： Pugh JC,Summers JW

    更新日期：1989-10-01 00:00:00

  • Effect of Ku80 depletion on the preintegrative steps of HIV-1 replication in human cells.

    abstract：:To gain new insights regarding the role of Ku, the DNA-PK DNA-binding component, during lentiviral DNA integration, we have investigated the HIV-1 replication in Ku80-depleted human cells. CEM4fx cells underexpressing the Ku80 factor were selected after transduction by a retroviral vector expressing the Ku80 full-leng...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2002.1515

    authors： Jeanson L,Subra F,Vaganay S,Hervy M,Marangoni E,Bourhis J,Mouscadet JF

    更新日期：2002-08-15 00:00:00

  • Geographic classification of dengue-2 virus strains by antigen signature analysis.

    abstract：:Dengue-2 virus strains from different locations were compared by T1-RNAse-resistant oligonucleotide fingerprinting and antigen signature analysis. The latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactiv...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90457-5

    authors： Monath TP,Wands JR,Hill LJ,Brown NV,Marciniak RA,Wong MA,Gentry MK,Burke DS,Grant JA,Trent DW

    更新日期：1986-10-30 00:00:00

  • Maize chlorotic mottle machlomovirus and wheat streak mosaic rymovirus concentrations increase in the synergistic disease corn lethal necrosis.

    abstract：:Corn lethal necrosis (CLN) is caused by the synergistic interaction between maize chlorotic mottle machlomovirus (MCMV) and any potyvirus which infects cereals. Interactions between MCMV and wheat streak mosaic rymovirus (WSMV) in N28Ht corn produced MCMV concentrations that averaged 3.3- to 11.2-fold higher in doubly...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8989

    authors： Scheets K

    更新日期：1998-03-01 00:00:00

  • Analysis of proviruses integrated in Fli-1 and Evi-1 regions in Cas-Br-E MuLV-induced non-T-, non-B-cell leukemias.

    abstract：:The DNAs of the Cas-Br-E MuLV-induced leukemias always contain somatically acquired mink cell focus-forming (MCF) recombinant proviruses. MCF recombinants could be involved during leukemogenesis at both preleukemic times and in late-stage tumors. Among the Cas-Br-E-induced non-T-, non-B-cell leukemias, viral integrati...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90241-g

    authors： Bergeron D,Poliquin L,Houde J,Barbeau B,Rassart E

    更新日期：1992-12-01 00:00:00

  • Lineage structures in the genome sequences of three Epstein-Barr virus strains.

    abstract：:Whole genome sequences for three Epstein-Barr virus strains (B95-8, GD1 and AG876) were aligned and compared. In addition to known variable loci (including type-specific alleles for the EBNA2, EBNA3A, EBNA3B and EBNA3C genes, plus the EBNA1 and LMP1 genes), seven large-scale regions of lower-level diversity were ident...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.009

    authors： McGeoch DJ,Gatherer D

    更新日期：2007-03-01 00:00:00

  • In vivo and in vitro infection with two different molecular clones of visna virus.

    abstract：:The behavior of two genetically different molecular clones of visna virus KV1772-kv72/67 and LV1-1KS1 was compared in vivo and in vitro. On intracerebral inoculation, clone KV1772-kv72/67 induced a similar response in five sheep as has already been reported with neurovirulent derivates of visna virus. Virus was freque...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8428

    authors： Torsteinsdóttir S,Agnarsdóttir G,Matthíasdóttir S,Rafnar B,Andrésdóttir V,Andrésson OS,Staskus K,Pétursson G,Pálsson PA,Georgsson G

    更新日期：1997-03-17 00:00:00

  • HIV-1 clones resistant to a small molecule CCR5 inhibitor use the inhibitor-bound form of CCR5 for entry.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) infection can be inhibited by small molecules that target the CCR5 coreceptor. Here, we describe some properties of clonal viruses resistant to one such inhibitor, SCH-D, using both chimeric, infectious molecular clones and Env-pseudotypes. Studies using combinations of CCR5...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.11.004

    authors： Pugach P,Marozsan AJ,Ketas TJ,Landes EL,Moore JP,Kuhmann SE

    更新日期：2007-04-25 00:00:00

  • Hsp90 is involved in pseudorabies virus virion assembly via stabilizing major capsid protein VP5.

    abstract：:Many viruses utilize molecular chaperone heat shock protein 90 (Hsp90) for protein folding and stabilization, however, the role of Hsp90 in herpesvirus lifecycle is obscure. Here, we provide evidence that Hsp90 participates in pseudorabies virus (PRV) replication. Viral growth kinetics assays show that Hsp90 inhibitor...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.10.013

    authors： Zhang WJ,Wang RQ,Li LT,Fu W,Chen HC,Liu ZF

    更新日期：2021-01-15 00:00:00

  • Neuronal IFN signaling is dispensable for the establishment of HSV-1 latency.

    abstract：:IFN responses control acute HSV infection, but their role in regulating HSV latency is poorly understood. To address this we used mice lacking IFN signaling specifically in neural tissues. These mice supported a higher acute viral load in nervous tissue and delayed establishment of latency. While latent HSV-1 genome c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.06.016

    authors： Rosato PC,Katzenell S,Pesola JM,North B,Coen DM,Leib DA

    更新日期：2016-10-01 00:00:00

  • Human papillomavirus type 16 E7 oncoprotein engages but does not abrogate the mitotic spindle assembly checkpoint.

    abstract：:The mitotic spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during mitosis by censoring kinetochore-microtubule interactions. It is frequently rendered dysfunctional during carcinogenesis causing chromosome missegregation and genomic instability. There are conflicting reports whether the HPV1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.06.006

    authors： Yu Y,Munger K

    更新日期：2012-10-10 00:00:00

  • GAG-binding variants of tick-borne encephalitis virus.

    abstract：:Previously different authors described various flavivirus mutants with high affinity to cell glycosaminoglycans and low neuroinvasiveness in mice that were obtained consequently passages in cell cultures or in ticks. In present study the analysis of TBEV isolates has shown existence of GAG-binding variants in natural ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.12.012

    authors： Kozlovskaya LI,Osolodkin DI,Shevtsova AS,Romanova LIu,Rogova YV,Dzhivanian TI,Lyapustin VN,Pivanova GP,Gmyl AP,Palyulin VA,Karganova GG

    更新日期：2010-03-15 00:00:00

  • Inhibition of uncoating of tobacco mosaic virus particles in protoplasts from transgenic tobacco plants that express the viral coat protein gene.

    abstract：:The uncoating of tobacco mosaic virus (TMV) particles in protoplasts isolated from leaves of transgenic tobacco plants that express the TMV coat protein gene was investigated. Extracts of these protoplasts collected up to 1 hr after inoculation with TMV contained fewer of the complexes ("striposomes") thought to be in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90163-l

    authors： Wu XJ,Beachy RN,Wilson TM,Shaw JG

    更新日期：1990-12-01 00:00:00

  • Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization.

    abstract：:RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9497

    authors： Navarro JA,Darós JA,Flores R

    更新日期：1999-01-05 00:00:00

  • Interaction of rotavirus cores with the nonstructural glycoprotein NS28.

    abstract：:The nonstructural rotavirus receptor glycoprotein NS28 is 175 amino acids long and oriented in the RER membrane with the NH2 terminus on the luminal side and approximately 131 amino acids accessible from the cytoplasmic side. Au et al. (1988) have demonstrated that NS28 is able to interact with rotavirus single-shelle...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90515-1

    authors： Meyer JC,Bergmann CC,Bellamy AR

    更新日期：1989-07-01 00:00:00

  • Analysis of nucleotides 13-96 of the human parainfluenza virus type 3 antigenomic promoter reveals positive- and negative-acting replication elements.

    abstract：:During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this st...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.08.003

    authors： Gander JR,Schwan LM,Hoffman MA

    更新日期：2011-10-25 00:00:00

  • Simvastatin and oseltamivir combination therapy does not improve the effectiveness of oseltamivir alone following highly pathogenic avian H5N1 influenza virus infection in mice.

    abstract：:Nonspecific anti-inflammatory drugs have been purported to reduce the burden of severe influenza disease. We demonstrate that, unlike oseltamivir administration, simvastatin administration did not reduce morbidity, mortality, or viral load of mice infected with H1N1 or H5N1 viruses. No added benefit to the efficacy of...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.01.017

    authors： Belser JA,Szretter KJ,Katz JM,Tumpey TM

    更新日期：2013-04-25 00:00:00

  • Enhanced infectivity of modified bluetongue virus particles for two insect cell lines and for two Culicoides vector species.

    abstract：:Previous studies (Mertens et al., Virology 157, 375-386, 1987) have shown that removal of the outer capsid layer from bluetongue virus (BTV) significantly reduces (approximately x 10(-4)) the infectivity of the resultant core particle for mammalian cells (BHK 21 cells). In contrast, the studies reported here, using a ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0153

    authors： Mertens PP,Burroughs JN,Walton A,Wellby MP,Fu H,O'Hara RS,Brookes SM,Mellor PS

    更新日期：1996-03-15 00:00:00

  • HPV-16 E5 down-regulates expression of surface HLA class I and reduces recognition by CD8 T cells.

    abstract：:HPV-16 is the major causes of cervical cancer. Persistence of infection is a necessary event for progression of the infection to cancer. Among other factors, virus persistence is due the viral proteins fighting the immune response. HPV-16 E5 down-regulates MHC/HLA class I, which is much reduced on the cell surface and...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.07.044

    authors： Campo MS,Graham SV,Cortese MS,Ashrafi GH,Araibi EH,Dornan ES,Miners K,Nunes C,Man S

    更新日期：2010-11-10 00:00:00

  • A long HBV transcript encoding pX is inefficiently exported from the nucleus.

    abstract：:The longest hepatitis B virus transcript is a 3.9-kb mRNA whose function remained unclear. In this study, we wished to identify the translation products and physiological role of this viral transcript. This transcript initiates from the X promoter region ignoring the inefficient and noncanonical viral polyadenylation ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00156-9

    authors： Doitsh G,Shaul Y

    更新日期：2003-05-10 00:00:00

  • The evolution and characterization of influenza A(H7N9) virus under the selective pressure of peramivir.

    abstract：:Human infection with H7N9 virus has provoked global public health concern due to the substantial morbidity and mortality. Neuraminidase inhibitors (NAIs) are used as first-line drugs to treat the infection. However, virus quasispecies can evolve rapidly under drug pressure, which may alter various biological character...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.08.004

    authors： Ge Y,Chi Y,Min X,Zhao K,Wu B,Wu T,Zhu X,Shi Z,Zhu F,Cui L

    更新日期：2019-10-01 00:00:00

  • Poultry vaccination directed evolution of H9N2 low pathogenicity avian influenza viruses in Korea.

    abstract：:Significant economic losses in the poultry industries have resulted from H9N2 low pathogenic avian influenza virus infections across North Africa, the Middle East and Asia. The present study investigated the evolutionary dynamics of H9N2 viruses circulating in Korea from 1996 to 2012. Our analysis of viral population ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.11.023

    authors： Lee DH,Fusaro A,Song CS,Suarez DL,Swayne DE

    更新日期：2016-01-15 00:00:00

  • Bipartite geminivirus host adaptation determined cooperatively by coding and noncoding sequences of the genome.

    abstract：:Bipartite geminiviruses are small, plant-infecting viruses with genomes composed of circular, single-stranded DNA molecules, designated A and B. Although they are closely related genetically, individual bipartite geminiviruses frequently exhibit host-specific adaptation. Two such viruses are bean golden mosaic virus (...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0620

    authors： Petty IT,Carter SC,Morra MR,Jeffrey JL,Olivey HE

    更新日期：2000-11-25 00:00:00

  • In vitro strain adaptation of CWD prions by serial protein misfolding cyclic amplification.

    abstract：:We used serial protein misfolding cyclic amplification (sPMCA) to amplify the D10 strain of CWD prions in a linear relationship over two logs of D10 dilutions. The resultant PMCA-amplified D10 induced terminal TSE disease in CWD-susceptible Tg(cerPrP)1536 mice with a survival time approximately 80 days shorter than th...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.09.023

    authors： Meyerett C,Michel B,Pulford B,Spraker TR,Nichols TA,Johnson T,Kurt T,Hoover EA,Telling GC,Zabel MD

    更新日期：2008-12-20 00:00:00

  • Recurrence of WHV integration in the b3n locus in woodchuck hepatocellular carcinoma.

    abstract：:Frequent occurrence of woodchuck hepatitis virus DNA (WHV DNA) integration into or in proximity to myc oncogenes and in the win locus of cellular genome in woodchuck hepatocellular carcinomas (HCC) has been described by several authors. We report a further cellular locus as a recurrent target for WHV integration in wo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.9936

    authors： Bruni R,Argentini C,D'Ugo E,Giuseppetti R,Ciccaglione AR,Rapicetta M

    更新日期：1995-12-01 00:00:00