Abstract:
:We used serial protein misfolding cyclic amplification (sPMCA) to amplify the D10 strain of CWD prions in a linear relationship over two logs of D10 dilutions. The resultant PMCA-amplified D10 induced terminal TSE disease in CWD-susceptible Tg(cerPrP)1536 mice with a survival time approximately 80 days shorter than the original D10 inoculum, similar to that produced by in vivo sub-passage of D10 in Tg(cerPrP)1536 mice. Both in vitro-amplified and mouse-passaged D10 produced brain lesion profiles, glycoform ratios and conformational stabilities significantly different than those produced by the original D10 inoculum in Tg(cerPrP)1536 mice. These findings demonstrate that sPMCA can amplify and adapt prion strains in vitro as effectively and much more quickly than in vivo strain adaptation by mouse passage. Thus sPMCA may represent a powerful tool to assess prion strain adaptation and species barriers in vitro.
journal_name
Virologyjournal_title
Virologyauthors
Meyerett C,Michel B,Pulford B,Spraker TR,Nichols TA,Johnson T,Kurt T,Hoover EA,Telling GC,Zabel MDdoi
10.1016/j.virol.2008.09.023subject
Has Abstractpub_date
2008-12-20 00:00:00pages
267-76issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(08)00604-1journal_volume
382pub_type
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