Abstract:
:The coat protein (CP) genes of all the strains of potato virus X (PVX) code for a protein of 25 kDa. Analysis of the CP by SDS-PAGE shows a migration mobility of 27 to 29 kDa, depending of the strain. Amino acid identity between some strains is too high to explain such abnormal migration by differences in primary structure of the protein. Periodate oxidation demonstrated the presence of carbohydrate moieties in purified CP of six PVX strains (cp, CP4, HB, MS, DX, CS35), and trifluoromethanesulfonic acid treatment identified glycosylated and nonglycosylated bands in SDS-PAGE complex patterns of CP and in Western blots. Digestion with glycosidases, recognition by lectins, and mild alkali treatment (beta-elimination) of PVX CP indicated the presence of an O-linked sugar.
journal_name
Virologyjournal_title
Virologyauthors
Tozzini AC,Ek B,Palva ET,Hopp HEdoi
10.1006/viro.1994.1386subject
Has Abstractpub_date
1994-08-01 00:00:00pages
651-8issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71386-9journal_volume
202pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that, following primary infection (varicella), establishes latency in sensory, autonomic, sympathetic and parasympathetic neurons, where it remains until reactivation (zoster). VZV-specific cell-mediated immune responses maintain VZV latency; thus, immunos...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.06.013
更新日期:2018-09-01 00:00:00
abstract::Segment 7 (M) of the cold-adapted live influenza A virus vaccine plays a primary role in the ability of this virus to interfere with the replication of wild-type influenza A viruses. This conclusion is based on several lines of evidence. Single gene reassortant viruses derived by crossing influenza A/Ann Arbor/6/60 (H...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90011-y
更新日期:1991-01-01 00:00:00
abstract::It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90239-1
更新日期:1980-10-15 00:00:00
abstract::Here we address the role of RIG-I and TLR3 in differential cytokine responses against Simian Virus 5 (SV5) and two distinct cytokine inducing SV5 mutants. IFN-beta and IL-6 secretion was induced by infection with P/V-CPI-, an SV5 mutant with P/V substitutions, and were reduced by either siRNA-mediated knockdown of RIG...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.014
更新日期:2010-02-05 00:00:00
abstract::The Nef protein of Simian immunodeficiency virus (SIV) associates with multiple T lymphocyte signaling proteins, including the T cell receptor (TCR) zeta chain. We demonstrate here that these interactions are conserved and highly specific. Nefs derived from genetically diverse strains of SIV (SIV(mac)239, SIV(smm)PBj,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1628
更新日期:2002-10-10 00:00:00
abstract::Monoclonal antibodies to the matrix or E1 glycoprotein of mouse hepatitis virus (MHV) were tested for their ability to protect mice from a normally lethal challenge of MHV-4. Four antibodies were tested, and two of these, J.1.3 and J.3.9, were protective. Protection did not correlate with virus neutralization in vitro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90415-7
更新日期:1989-01-01 00:00:00
abstract::Cells infected with a reassortant (113/Ho, H7N2) between A/fowl plague/Rostock/34 (FPV, H7N1) and A/Hong Kong/1/68 (H3N2) carrying RNA segments 1 and 6 of the Hong Kong virus and the residual genes of FPV, synthesized at 40 degrees a neuraminidase (NA) which is enzymatically not active and which is not incorporated in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90266-7
更新日期:1986-04-15 00:00:00
abstract::A cDNA encoding the entire amino acid sequence of the G glycoprotein of respiratory syncytial virus (RSV) was inserted into a yeast-Escherichia coli shuttle vector such that expression of the virus gene was regulated by the yeast GAL1 promoter. Transformation of Saccharomyces cerevisiae with the vector led to the form...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90485-5
更新日期:1987-08-01 00:00:00
abstract::The bipartite genomic RNAs of red clover necrotic mosaic virus (RCNMV) lack a 5' cap and a 3' poly(A) tail. RNA1 encodes viral replication proteins, and RNA2 encodes a movement protein (MP). These proteins are translated in a cap-independent manner. We previously identified two cis-acting RNA elements that cooperative...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.06.015
更新日期:2017-09-01 00:00:00
abstract::We expressed the carboxy-terminal portion of the E2 open reading frame (ORF)-encoded protein of human papillomavirus type 16 (HPV-16) and purified it to near homogeneity. Using DNase I footprinting techniques, we show that like the homologous protein from bovine papillomavirus type 1 (BPV-1), HPV-18, HPV-11, it binds ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90109-5
更新日期:1990-02-01 00:00:00
abstract::The ability of HIV to match levels of viral mRNA to the activation state of the host cell may play a role in its ability to persist as well as to replicate. This linkage depends on the function of the viral transcriptional regulatory protein, Tat, which increases the efficiency of RNA elongation (transcriptional proce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9647
更新日期:1999-05-10 00:00:00
abstract::The yellow fever 17D virus is attenuated and used for human vaccination. Two of its substrains, 17D-204 and 17DD, are used for vaccine production. One of the remarkable properties of this vaccine is limited viral replication in the host but with significant dissemination of the viral mass, yielding a robust and long-l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00583-x
更新日期:2003-11-10 00:00:00
abstract::The co-existence of hepatitis B surface antigen (HBsAg) and anti-HBs antibodies (HBsAb) in serum of hepatitis B virus (HBV)-chronic carriers has been previously associated with HBsAg-amino acid (aa) substitutions. However, the aa pattern of HBV-reverse transcriptase (RT) and the clinical settings associated with this ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.012
更新日期:2007-10-10 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) Rev is a 19-kDa regulatory protein which binds to unspliced and partially spliced HIV-1 RNAs. Export, splicing, stability, and translation of HIV-1 RNAs are influenced by Rev. To further understand the effect of Rev on HIV-1 RNA splicing, the intranuclear localization of uns...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9312
更新日期:1998-09-30 00:00:00
abstract::Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in cont...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.12.018
更新日期:2016-03-01 00:00:00
abstract::Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.008
更新日期:2014-09-01 00:00:00
abstract::During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.08.007
更新日期:2007-12-20 00:00:00
abstract::Immunodeficiency caused by HIV infection probably results from profound dysregulation of normal T lymphocyte properties by the virus. Despite description of the virus cytopathicity and numerous modifications in T cell functions, such as perturbation of antigen receptor signaling, CD4 downregulation, and induction of a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1124
更新日期:1994-03-01 00:00:00
abstract::Genetic studies have been carried out on orbiviruses in the Great Island (GI) antigenic subgroup of the Kemerovo serogroup (Orbivrus, Reoviridae) to elucidate the functions of the 10 genomic double-stranded RNA segments. Such studies have shown that segment 4 is the major genetic determinant of neurovirulence (P.A. Nu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90096-a
更新日期:1990-02-01 00:00:00
abstract::There is growing evidence that the receptor-binding characteristics of influenza viruses are affected by the host-dependent glycosylation of viral hemagglutinin (HA). To better understand these effects, we propagated two variants of the human influenza virus USSR/90/77 (which differed by the mutation Asn131 reversible...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9224
更新日期:1998-08-01 00:00:00
abstract::The post-entry events of HIV-1 infection occur within reverse transcription complexes derived from the viral cores entering the target cell. HIV-1 cores contain host proteins incorporated from virus-producing cells. In this report, we show that MCM5, a subunit of the hexameric minichromosome maintenance (MCM) DNA heli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.023
更新日期:2016-10-01 00:00:00
abstract::The polyomavirus hr-t class of mutants has served as a major prototype to study the function of middle T + small T in the virus lytic cycle, Biochem. Biophys. Acta 695 (2), 69-95). The properties of these middle T + small T defective mutants were defined by comparisons with "wild-type" strains reconstructed by marker ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00030-2
更新日期:2003-03-15 00:00:00
abstract::Published ultraviolet (uv) inactivation data and in vitro transcription studies have suggested that vesicular stomatitis virus (VSV) leader RNA was solely responsible for the inhibition of host cell RNA synthesis by this virus. Since no protein product is encoded in leader RNA, this conclusion implied that no protein ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90448-9
更新日期:1985-01-15 00:00:00
abstract::The molecular cloning of the DNA provirus of Mason-Pfizer monkey virus (M-PMV) is described. Fourteen independent clones of integrated M-PMV proviruses were isolated from a human embryo kidney cell line that had been previously derived from a single cell clone infected with M-PMV. Characterization of these clones for ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90023-1
更新日期:1986-09-01 00:00:00
abstract::Arabis mosaic virus is a subgroup A nepovirus. The RNA1-encoded polyprotein (P1) contains the domains for the NTP-binding protein (NTB), VPg, proteinase (Pro) and polymerase at its C-terminus. Putative cleavage sites delineating these domains have been proposed. However, the number and location of cleavage sites upstr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.01.046
更新日期:2008-05-25 00:00:00
abstract::The enteric adenovirus type 40 strain Dugan (Ad40) cannot be passaged in HeLa cells, but will grow in 293 cells, which express Ad5 E1 functions. To determine the reason for this limited host range, KB cell lines expressing Ad2 E1A, E1B, or E1A + E1B (L. E. Babiss, C. S. H. Young, P. B. Fisher, and H. S. Ginsberg, 1983...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90634-x
更新日期:1989-08-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.001
更新日期:2013-02-05 00:00:00
abstract::CD8+ T lymphocytes are critical for the control of gammaherpesvirus latency. To determine how memory CD8+ T cells generated during latency differ from those primed during acute or chronic viral infection, we adoptively transferred naive P14 CD8+ T cells into uninfected recipients, and examined surface proteins, cytoki...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.06.018
更新日期:2017-09-01 00:00:00
abstract::Whole genome sequences for three Epstein-Barr virus strains (B95-8, GD1 and AG876) were aligned and compared. In addition to known variable loci (including type-specific alleles for the EBNA2, EBNA3A, EBNA3B and EBNA3C genes, plus the EBNA1 and LMP1 genes), seven large-scale regions of lower-level diversity were ident...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.009
更新日期:2007-03-01 00:00:00
abstract::Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discov...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9893
更新日期:1999-10-10 00:00:00