当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > The requirement of protein synthesis for VSV inhibition of host cell RNA synthesis.

The requirement of protein synthesis for VSV inhibition of host cell RNA synthesis.

Abstract：

:Published ultraviolet (uv) inactivation data and in vitro transcription studies have suggested that vesicular stomatitis virus (VSV) leader RNA was solely responsible for the inhibition of host cell RNA synthesis by this virus. Since no protein product is encoded in leader RNA, this conclusion implied that no protein synthesis should be required for this effect. Therefore, the inhibitory activity of VSV was examined in the presence of the protein synthesis inhibitors, cycloheximide, pactamycin, and emetine. Protein synthesis inhibitors are known not to interfere with VSV primary transcription, but in their presence viral replication and amplification of transcription do not take place. Although at 39 degrees the VSV mutant tsG22 could undergo only primary transcription, maximum inhibition of host cell RNA synthesis took place. However, in the presence of the protein synthesis inhibitors the VSV mutant was no longer able to interfere with host cell RNA synthesis. These results could not be explained by a change in the concentration of intracellular leader RNA which remained unaltered by the drugs. Similar results were also obtained with wild-type VSV in the presence of cycloheximide. Upon removal of the drug, inhibition of host cell RNA synthesis was reestablished in parallel with the restoration of protein synthesis. It is concluded that protein synthesis is required for the inhibitory activity of VSV, presumably because the active inhibitory complex is a nucleoprotein containing leader RNA and either a cellular protein or the viral N protein. The cellular protein would have to be in limiting supply since de novo protein synthesis was required for the inhibition to take place.

journal_name

Virology

journal_title

Virology

authors

Poirot MK,Schnitzlein WM,Reichmann ME

doi

10.1016/0042-6822(85)90448-9

subject

Has Abstract

pub_date

1985-01-15 00:00:00

pages

91-101

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

140

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Interferon-inducible gene expression in chimpanzee liver infected with hepatitis C virus.

    abstract：:The molecular host response to hepatitis C virus (HCV) infection was examined by isolation of HCV-induced genes from a cDNA library constructed from chimpanzee liver during the acute phase of hepatitis C. Two cDNA clones, 130-7 and 130-51, were obtained by differential hybridization with cDNA probes prepared from poly...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90925-f

    authors： Kato T,Esumi M,Yamashita S,Abe K,Shikata T

    更新日期：1992-10-01 00:00:00

  • Effect of exogenous interferon and an interferon inducer on western equine encephalitis virus disease in a hamster model.

    abstract：:Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.031

    authors： Julander JG,Siddharthan V,Blatt LM,Schafer K,Sidwell RW,Morrey JD

    更新日期：2007-04-10 00:00:00

  • RFHVMn ORF73 is structurally related to the KSHV ORF73 latency-associated nuclear antigen (LANA) and is expressed in retroperitoneal fibromatosis (RF) tumor cells.

    abstract：:Retroperitoneal fibromatosis herpesvirus (RFHV), the macaque homolog of the human rhadinovirus, Kaposi's sarcoma-associated herpesvirus (KSHV), was first identified in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS. We cloned and sequenced the ORF73 latency-associated nuclear antigen (LAN...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.06.022

    authors： Burnside KL,Ryan JT,Bielefeldt-Ohmann H,Gregory Bruce A,Thouless ME,Tsai CC,Rose TM

    更新日期：2006-10-10 00:00:00

  • Marek's disease virus-encoded analog of microRNA-155 activates the oncogene c-Myc by targeting LTBP1 and suppressing the TGF-β signaling pathway.

    abstract：:Marek's disease virus (MDV) is a representative alpha herpes virus able to induce rapid-onset T-cell lymphoma in its natural host and regarded as an ideal model for the study of virus-induced tumorigenesis. Recent studies have shown that the mdv1-miR-M4-5p, a viral analog of cellular miR-155, is critical for MDV׳s onc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.11.027

    authors： Chi JQ,Teng M,Yu ZH,Xu H,Su JW,Zhao P,Xing GX,Liang HD,Deng RG,Qu LH,Zhang GP,Luo J

    更新日期：2015-02-01 00:00:00

  • The RNA encompassing the internal ribosome entry site in the poliovirus 5' nontranslated region enhances the encapsidation of genomic RNA.

    abstract：:Poliovirus replicons were constructed which contain the internal ribosome entry site (IRES) of encephalomyocarditis virus (EMCV) substituted for the poliovirus IRES. To monitor gene expression and encapsidation, the gene encoding firefly luciferase was substituted for the P1 gene. Replicons can be encapsidated followi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0433

    authors： Johansen LK,Morrow CD

    更新日期：2000-08-01 00:00:00

  • Pirates of the Caudovirales.

    abstract：:Molecular piracy is a biological phenomenon in which one replicon (the pirate) uses the structural proteins encoded by another replicon (the helper) to package its own genome and thus allow its propagation and spread. Such piracy is dependent on a complex web of interactions between the helper and the pirate that occu...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2012.10.028

    authors： Christie GE,Dokland T

    更新日期：2012-12-20 00:00:00

  • Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV.

    abstract：:Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8457

    authors： Gomi S,Zhou CE,Yih W,Majima K,Maeda S

    更新日期：1997-03-31 00:00:00

  • Amplification of the chromosome 20q region is associated with expression of HPV-16 E7 in human airway and anogenital epithelial cells.

    abstract：:To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.06.027

    authors： Klingelhutz AJ,Qian Q,Phillips SL,Gourronc FA,Darbro BW,Patil SR

    更新日期：2005-09-30 00:00:00

  • Protection against polyoma virus-induced tumors is perforin-independent.

    abstract：:CD8 T cells are necessary for controlling tumors induced by mouse polyoma virus (PyV), but the effector mechanism(s) responsible have not been determined. We examined the PyV tumorigenicity in C57BL/6 mice mutated in Fas or carrying targeted disruptions in the perforin gene or in both TNF receptor type I and type II g...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.08.044

    authors： Byers AM,Hadley A,Lukacher AE

    更新日期：2007-02-20 00:00:00

  • Microevolution of tick-borne encephalitis virus in course of host alternation.

    abstract：:Two tick-borne encephalitis (TBE) virus variants were studied: mouse brain-adapted strain EK-328 and its derivate adapted to Hyalomma marginatum ticks. The tick-adapted virus exhibited small-plaque phenotype and slower replication in PEK cells, higher yield in ticks, decreased neuroinvasiveness in mice, increased bind...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.12.013

    authors： Romanova LIu,Gmyl AP,Dzhivanian TI,Bakhmutov DV,Lukashev AN,Gmyl LV,Rumyantsev AA,Burenkova LA,Lashkevich VA,Karganova GG

    更新日期：2007-05-25 00:00:00

  • Morphogenesis of filamentous bacteriophage f1: orientation of extrusion and production of polyphage.

    abstract：:Crosslinking reagents were used to interrupt the process of filamentous phage morphogenesis and investigate the orientation in which nascent virions are extruded through the host cell membrane. Infected bacteria with emerging phage particles were crosslinked with glutaraldehyde. Immunoferritin-labeling studies on thes...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90382-3

    authors： Lopez J,Webster RE

    更新日期：1983-05-01 00:00:00

  • An analysis of the requirements for human cytomegalovirus oriLyt-dependent DNA synthesis in the presence of the herpes simplex virus type 1 replication fork proteins.

    abstract：:Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00005-9

    authors： Reid GG,Ellsmore V,Stow ND

    更新日期：2003-04-10 00:00:00

  • Evidence that RNA 4 of alfalfa mosaic virus does not replicate autonomously.

    abstract：:A mutant (Tbts7) of alfalfa mosaic virus, the coat protein of which is unable to activate the viral genome (the RNA species 1, 2, and 3, which need some coat protein for infectivity) at 30 degrees , can be rescued at this temperature by adding to the inoculum wild-type RNA 3 (the genome part that contains the coat pro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(82)90528-1

    authors： Smit CH,Jaspars EM

    更新日期：1982-02-01 00:00:00

  • Recombinant adeno-associated virus expressing the receptor-binding domain of severe acute respiratory syndrome coronavirus S protein elicits neutralizing antibodies: Implication for developing SARS vaccines.

    abstract：:Development of an effective vaccine for severe acute respiratory syndrome (SARS) remains to be a priority to prevent possible re-emergence of SARS coronavirus (SARS-CoV). We previously demonstrated that the receptor-binding domain (RBD) of SARS-CoV S protein is a major target of neutralizing antibodies. This suggests ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.03.049

    authors： Du L,He Y,Wang Y,Zhang H,Ma S,Wong CK,Wu SH,Ng F,Huang JD,Yuen KY,Jiang S,Zhou Y,Zheng BJ

    更新日期：2006-09-15 00:00:00

  • Inhibition of vaccinia virus replication by N-(phosphonoacetyl)-L-aspartate: differential effects on viral gene expression result from a reduced pyrimidine nucleotide pool.

    abstract：:The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8735

    authors： Katsafanas GC,Grem JL,Blough HA,Moss B

    更新日期：1997-09-15 00:00:00

  • A chimpanzee adenoviral vector-based rabies vaccine protects beagle dogs from lethal rabies virus challenge.

    abstract：:Rabies continues to poses serious threats to the public health in many countries. The development of novel inexpensive, safe and effective vaccines has become a high priority for rabies control worldwide. We previously generated a novel recombinant rabies vaccine by cloning rabies virus glycoprotein into a chimpanzee ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.07.022

    authors： Wang X,Fang Z,Xiong J,Yang K,Chi Y,Tang X,Ma L,Zhang R,Deng F,Lan K,Zhou D

    更新日期：2019-10-01 00:00:00

  • Unique physicochemical properties of human enteric Ad41 responsible for its survival and replication in the gastrointestinal tract.

    abstract：:Human enteric adenovirus Ad41 is associated with children gastroenteritis. To infect gastrointestinal cells, the invading virus must be acid-stable and resistant to inactivation by bile salts and proteases. In addition, it has to cross the mucus barrier before it infects mucosa cells. We show that Ad41 infectivity is ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.01.020

    authors： Favier AL,Burmeister WP,Chroboczek J

    更新日期：2004-04-25 00:00:00

  • Human papillomavirus type 2c is identical to human papillomavirus type 27.

    abstract：:Human papillomavirus type 27 (HPV-27) and HPV-2c are isolates of the same virus as judged by genomic sequence identity. In a 400-bp stretch of the long control region and the E6 and L1 genes no changes were found between HPV-27 and HPV-2c whereas 55 changes were found between HPV-27 and HPV-2a. This justifies the elev...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1307

    authors： Chan SY,Tan CH,Delius H,Bernard HU

    更新日期：1994-06-01 00:00:00

  • TGF-β regulation of gene expression at early and late stages of HPV16-mediated transformation of human keratinocytes.

    abstract：:In our in vitro model for HPV16-mediated transformation, HPV16-immortalized human keratinocytes (HKc/HPV16) give rise to differentiation resistant, premalignant cells (HKc/DR). HKc/DR, but not HKc/HPV16, are resistant to growth inhibition by transforming growth factor beta (TGF-β), due to a partial loss of TGF-β recep...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.08.034

    authors： Kowli S,Velidandla R,Creek KE,Pirisi L

    更新日期：2013-12-01 00:00:00

  • Infection and uptake of duck hepatitis B virus by duck hepatocytes maintained in the presence of dimethyl sulfoxide.

    abstract：:Primary duck hepatocytes maintained in serum-free culture medium containing dimethyl sulfoxide support efficient replication of duck hepatitis B virus following infection in vitro. Cells remain susceptible to infection for at least 2 weeks after plating, allowing spread of virus via repeat cycles of infection. Up to 1...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90199-2

    authors： Pugh JC,Summers JW

    更新日期：1989-10-01 00:00:00

  • Association of toll-like receptor polymorphisms with susceptibility to chikungunya virus infection.

    abstract：:Chikungunya virus (CHIKV) infection leads to activation of innate immune response by triggering Toll-like receptor (TLR) pathways resulting in elevated cytokines and type-I interferon levels. Genetic variations of these genes may influence human CHIKV-susceptibility and disease progression. Present study aimed to iden...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.08.009

    authors： Dutta SK,Tripathi A

    更新日期：2017-11-01 00:00:00

  • Development of plaque assays for hepatitis C virus-JFH1 strain and isolation of mutants with enhanced cytopathogenicity and replication capacity.

    abstract：:HCV culture in vitro results in massive cell death, which suggests the presence of HCV-induced cytopathic effects. Therefore, we investigated its mechanisms and viral nucleotide sequences involved in this effect using HCV-JFH1 cell culture and a newly developed HCV plaque assay technique. The plaque assay developed cy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.09.019

    authors： Sekine-Osajima Y,Sakamoto N,Mishima K,Nakagawa M,Itsui Y,Tasaka M,Nishimura-Sakurai Y,Chen CH,Kanai T,Tsuchiya K,Wakita T,Enomoto N,Watanabe M

    更新日期：2008-02-05 00:00:00

  • Cytosine deamination and selection of CpG suppressed clones are the two major independent biological forces that shape codon usage bias in coronaviruses.

    abstract：:Using the complete genome sequences of 19 coronavirus genomes, we analyzed the codon usage bias, dinucleotide relative abundance and cytosine deamination in coronavirus genomes. Of the eight codons that contain CpG, six were markedly suppressed. The mean NNU/NNC ratio of the six amino acids using either NNC or NNU as ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.08.010

    authors： Woo PC,Wong BH,Huang Y,Lau SK,Yuen KY

    更新日期：2007-12-20 00:00:00

  • The spiroplasma virus 4 replicative form cloned in Escherichia coli transfects spiroplasmas.

    abstract：:The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90060-7

    authors： Pascarel-Devilder MC,Renaudin J,Bove JM

    更新日期：1986-06-01 00:00:00

  • Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses.

    abstract：:A full-size cloned cDNA copy of the rotavirus gene encoding the structural neutralization glycoprotein (VP7) of Nebraska calf diarrhea virus (NCDV), a strain recently shown to be effective as a vaccine in children, has been sequenced. Comparison of the deduced amino acid sequence of NCDV (serotype 6) VP7 with that of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90260-0

    authors： Glass RI,Keith J,Nakagomi O,Nakagomi T,Askaa J,Kapikian AZ,Chanock RM,Flores J

    更新日期：1985-03-01 00:00:00

  • Glycosylation of the major outer capsid protein of bluetongue viruses.

    abstract：:The major outer capsid protein, VP5, of five US bluetongue viruses, was found to be glycosylated. Enzymatic removal of the carbohydrate moiety did not affect the electrophoretic mobility of VP5 in SDS-PAGE, indicating the presence of only short and possibly unbranched oligosaccharide chains. The surface accessibilitie...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(83)90000-4

    authors： Yang YY,Li JK

    更新日期：1993-05-01 00:00:00

  • Heat shock protein 90AB1 and hyperthermia rescue infectivity of HIV with defective cores.

    abstract：:We previously showed that reduced infectivity of HIV with incompletely processed capsid-spacer protein 1 (CA-SP1) is rescued by cellular activation or increased expression of HSP90AB1, a member of the cytosolic heat shock protein 90 family. Here we show that HSP90AB1 is present in HIV virions and that HSP90AB1, but no...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.11.005

    authors： Joshi P,Sloan B,Torbett BE,Stoddart CA

    更新日期：2013-02-05 00:00:00

  • Reovirus type 3 synthesizes proteins in interferon-treated HeLa cells without reversing the antiviral state.

    abstract：:Treatment of HeLa cells with human lymphoblastoid interferon (IFN-alpha) does not inhibit reovirus type 3 protein synthesis during virus infection. In contrast, reovirus translation is blocked by treatment of L cells with mouse IFN-alpha. The (2'-5')A synthetase activity is induced in HeLa cells by IFN-alpha treatment...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90555-7

    authors： Feduchi E,Esteban M,Carrasco L

    更新日期：1988-06-01 00:00:00

  • Begomovirus genetic diversity in the native plant reservoir Solanum nigrum: Evidence for the presence of a new virus species of recombinant nature.

    abstract：:We examined the native plant host Solanum nigrum as reservoir of genetic diversity of begomoviruses that cause the tomato yellow leaf curl disease (TYLCD) emerging in southern Spain. Presence of isolates of all the species and strains found associated with TYLCD in this area was demonstrated. Mixed infections were com...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.02.028

    authors： García-Andrés S,Monci F,Navas-Castillo J,Moriones E

    更新日期：2006-07-05 00:00:00

  • West Nile virus infection of Drosophila melanogaster induces a protective RNAi response.

    abstract：:To determine if West Nile virus (WNV) infection of insect cells induces a protective RNAi response, Drosophila melanogaster S2 and Aedes albopictus C6/36 cells were infected with WNV, and the production of WNV-homologous small RNAs was assayed as an indicator of RNAi induction. A distinct population of approximately 2...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.04.021

    authors： Chotkowski HL,Ciota AT,Jia Y,Puig-Basagoiti F,Kramer LD,Shi PY,Glaser RL

    更新日期：2008-07-20 00:00:00