Abstract:
:The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI restriction site and has been cloned in Escherichia coli HB101 using the plasmid pBR328 as a vector. The recombinant plasmid was purified by equilibrium centrifugation in ethidium bromide-cesium chloride gradient. After ClaI endonuclease digestion, the inserted SpV4 RF DNA was recovered by low melting point agarose gel electrophoresis and was recircularized by ligation. The cloned SpV4 RF DNA was demonstrated to be infectious by transfection.
journal_name
Virologyjournal_title
Virologyauthors
Pascarel-Devilder MC,Renaudin J,Bove JMdoi
10.1016/0042-6822(86)90060-7subject
Has Abstractpub_date
1986-06-01 00:00:00pages
390-3issue
2eissn
0042-6822issn
1096-0341journal_volume
151pub_type
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