Escape from neutralization by the respiratory syncytial virus-specific neutralizing monoclonal antibody palivizumab is driven by changes in on-rate of binding to the fusion protein.

abstract：:The N-terminal portion of the reovirus cell attachment protein sigma 1 has recently been shown to possess intrinsic oligomerization and virion-anchoring functions. Sequence analysis of the sigma 1 proteins of the three reovirus serotypes has revealed the presence of distinct structural domains within this region: a te...

journal_title：Virology

authors： Leone G,Mah DC,Lee PW

更新日期：1991-05-01 00:00:00

abstract：:The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates...

journal_title：Virology

authors： Cheung AK

更新日期：2015-07-01 00:00:00

abstract：:Bluetongue virus (BTV) VP1 protein, a component of the viral RNA-directed RNA polymerase, but not the VP4 or VP6 proteins, was specifically incorporated into baculovirus expressed BTV core-like particles (composed of VP3 and VP7) and BTV virus-like particles (composed of VP2, VP3, VP5, and VP7). The VP1 protein has be...

journal_title：Virology

authors： Loudon PT,Roy P

更新日期：1991-02-01 00:00:00

abstract：:Zika virus (ZIKV) nonstructural protein 5 (NS5) is a multifunctional protein possessing methyltransferase and RNA-dependent RNA polymerase activities. In the present study, we have carried out an extensive mutagenesis analysis to determine the importance of nuclear localization sequences (NLS) of NS5 in its nuclear ac...

journal_title：Virology

authors： Ji W,Luo G

更新日期：2020-02-01 00:00:00

abstract：:Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deep...

journal_title：Virology

authors： Arroyo Mühr LS,Bzhalava D,Lagheden C,Eklund C,Johansson H,Forslund O,Dillner J,Hultin E

更新日期：2015-11-01 00:00:00

abstract：:Murine monoclonal antibodies were developed against the protein products produced by murine C127 cells which had been transfected with a recombinant plasmid clone containing the human T-cell leukemia (lymphotropic) virus type I (HTLV-I) proviral DNA coding regions for part of env, px, and the 3' LTR. Four antibodies w...

journal_title：Virology

authors： Newman MJ,Baker IT,Reitz MS,Eiden M,Blattner WA,Gallo RC,Mann DL

更新日期：1986-04-15 00:00:00

abstract：:The potential role(s) of cytokines in the reduction of infectious virus and persistent viral infection in the central nervous system was examined by determining the kinetics of cytokine mRNA expression following infection with the neurotropic JHM strain of mouse hepatitis virus. Mice were infected with an antibody esc...

journal_title：Virology

authors： Parra B,Hinton DR,Lin MT,Cua DJ,Stohlman SA

更新日期：1997-07-07 00:00:00

abstract：:The effect of adenovirus infection or transformation on the DNA replication of Minute Virus of Mice (MVM) was studied in human fibroblast cell lines. In WI38, HeLa, and 293 cells MVM infection allowed production of viral NS-1 and capsid proteins with or without adenovirus 2 (Ad2) co-infection. However, MVM DNA replica...

journal_title：Virology

authors： Fox E,Moen PT Jr,Bodnar JW

更新日期：1990-06-01 00:00:00

abstract：:The guinea pig cytomegalovirus (GPCMV) co-linear gene and potential functional homolog of HCMV UL84 (GP84) was investigated. The GP84 gene had delayed early transcription kinetics and transient expression studies of GP84 protein (pGP84) demonstrated that it targeted the nucleus and co-localized with the viral DNA poly...

journal_title：Virology

authors： McGregor A,Choi KY,Schleiss MR

更新日期：2011-02-05 00:00:00

abstract：:Five monoclonal antibodies (MAbs) to porcine group (gp) C rotaviruses (Cowden and Ah strains) reactive with both gp A and C rotaviruses in cell culture immunofluorescence (CCIF) tests were produced and characterized. These MAbs reacted with three strains of gp A and two strains of gp C rotaviruses in a CCIF test and w...

journal_title：Virology

authors： Tsunemitsu H,Ojeh CK,Jiang B,Simkins RA,Weilnau PA,Saif LJ

更新日期：1992-11-01 00:00:00

abstract：:Flaviviruses are RNA viruses that constitute a worrisome threat to global human and animal health. In Europe, West Nile virus (WNV) outbreaks have dramatically increased in number and severity in recent years, with dozens of human and horse deaths and a high avian mortality across the continent. Besides WNV, the only ...

journal_title：Virology

authors： Blázquez AB,Escribano-Romero E,Martín-Acebes MA,Petrovic T,Saiz JC

更新日期：2015-08-01 00:00:00

abstract：:Infection with many viruses results in the selective shutoff of host protein synthesis. A common target for virus interference with host protein synthesis is the cap-binding protein complex, eIF4F. The large subunit of the complex, eIF4G, is cleaved upon picornavirus (except cardiovirus) infection. Infection with aden...

journal_title：Virology

authors： Gingras AC,Sonenberg N

更新日期：1997-10-13 00:00:00

abstract：:Infection of adherent cell monolayers using a liquid inoculum represents an established method to reliably and quantitatively study virus infection, but poorly recapitulates the exposure and infection of cells in the respiratory tract that occurs during infection with aerosolized pathogens. To better simulate natural ...

journal_title：Virology

authors： Creager HM,Zeng H,Pulit-Penaloza JA,Maines TR,Tumpey TM,Belser JA

更新日期：2017-01-01 00:00:00

abstract：:An essentially complete DNA copy of the ambisense S RNA species of Punta Toro (PT) phlebovirus (T. Ihara, H. Akashi, and D.H.L. Bishop, 1984, Virology 136, 293-306) has been inserted in either orientation into Autographa californica nuclear polyhedrosis baculovirus (AcNPV) in lieu of the 5' coding region of the AcNPV ...

journal_title：Virology

authors： Overton HA,Ihara T,Bishop DH

更新日期：1987-04-01 00:00:00

abstract：:Genetically engineered mice with targeted mutations in genes encoding immunologically relevant molecules were used to elucidate the role of different immune effector mechanisms in protection against a rabies virus (RV) infection. In vaccinated animals challenged with a highly virulent strain of RV, antibodies were cru...

journal_title：Virology

authors： Xiang ZQ,Knowles BB,McCarrick JW,Ertl HC

更新日期：1995-12-20 00:00:00

abstract：:Edible vaccines that can be made widely available and easily administered could bring great benefit to the worldwide battle against pandemic viral infections. They can be used not only for the vaccination of humans and domesticated animals, but also for wild herds and live stock which are otherwise difficult to vaccin...

journal_title：Virology

authors： Lei H,Xu Y,Chen J,Wei X,Lam DM

更新日期：2010-11-25 00:00:00

abstract：:The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...

journal_title：Virology

authors： Porter DC,Ansardi DC,Wang J,McPherson S,Moldoveanu Z,Morrow CD

更新日期：1998-03-30 00:00:00

abstract：:To begin to map functional domains of the Sendai P-L RNA polymerase complex we wanted to characterize the P binding site on the Sendai L protein. Analysis of in vitro and in vivo P-L polymerase complex formation with carboxyl-truncations of the L protein showed that the N-terminal half of the protein was required. Sit...

journal_title：Virology

authors： Chandrika R,Horikami SM,Smallwood S,Moyer SA

更新日期：1995-11-10 00:00:00

abstract：:The nucleotide sequence of the env gene of a previously described human provirus (ERV3) has been determined beginning near the 3'-end of the pol gene and continuing through the 3'-LTR. Analysis of the nucleotide sequence revealed the presence of a long open reading frame of 1944 nucleotides that is capable of encoding...

journal_title：Virology

authors： Cohen M,Powers M,O'Connell C,Kato N

更新日期：1985-12-01 00:00:00

abstract：:The bovine herpesvirus 1 (BHV-1) U(S) ORF8 protein with homology to the Us9 protein of other alphaherpesviruses induces apoptosis in rabbit kidney (RK13) cells without the presence of other BHV-1-encoded proteins. In this article, we have characterized the cytotoxicity and growth behavior of a BHV-1 recombinant, BHV-1...

journal_title：Virology

authors： Nakamichi K,Matsumoto Y,Otsuka H

更新日期：2002-12-05 00:00:00

abstract：:We have previously shown that the Edmonston strain of measles virus enters and is released preferentially at the apical surfaces of polarized epithelial cells. Small amounts of virus were found to be released at the basal surface. In the present study, we passaged the virus in polarized cells and characterized the pas...

journal_title：Virology

authors： Blau DM,Compans RW

更新日期：1997-05-12 00:00:00

abstract：:Determinants of temperature sensitivity and/or attenuation in Sabin type 1 poliovirus reside in the 5' NTR and coding sequences of the capsid proteins and viral RNA polymerase, 3D(pol). Previous studies have implicated at least two mutations in 3D(pol) of Sabin 1 vaccine strain [PV1(S)], including a Y73H change, as co...

journal_title：Virology

authors： Paul AV,Mugavero J,Yin J,Hobson S,Schultz S,van Boom JH,Wimmer E

更新日期：2000-06-20 00:00:00

abstract：:The surface protein of Marburg virus (GP) is modified by acylation, as shown by labeling with [3H]myristic and [3H]palmitic acid. Acylation of GP also occurred when it was expressed in insect cells with the baculovirus expression system. Gas chromatographic analyses of the bound fatty acids indicated that exogenously ...

journal_title：Virology

authors： Funke C,Becker S,Dartsch H,Klenk HD,Mühlberger E

更新日期：1995-04-01 00:00:00

abstract：:Angiotensin converting enzyme 2 (ACE2) is the receptor that severe acute respiratory syndrome coronavirus (SARS-CoV) utilizes for target cell entry and, therefore, plays an important role in SARS pathogenesis. Since Chinese rhesus (rh) macaques do not usually develop SARS after SARS-CoV infection, it has been suggeste...

journal_title：Virology

authors： Chen Y,Liu L,Wei Q,Zhu H,Jiang H,Tu X,Qin C,Chen Z

更新日期：2008-11-10 00:00:00

abstract：:Peste des petits ruminants (PPR) is a viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. The virus is a member of the family Paramyxoviridae and the genus Morbillivirus. The disease has high morbidity and mortality rates and has a substantial economic impact in developing c...

journal_title：Virology

authors： Ismail TM,Yamanaka MK,Saliki JT,el-Kholy A,Mebus C,Yilma T

更新日期：1995-04-20 00:00:00

abstract：:Two genetically distinct infectious recombinant virus clones (pMLV, constructed from Ingelvac PRRS MLV and pMN184, constructed from virulent strain MN184) were developed to study attenuation of contemporary porcine reproductive and respiratory syndrome virus (PRRSV) strain MN184. Two reciprocal chimeric clones (pMLVOR...

journal_title：Virology

authors： Wang Y,Liang Y,Han J,Burkhart KM,Vaughn EM,Roof MB,Faaberg KS

更新日期：2008-02-20 00:00:00

abstract：:The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...

journal_title：Virology

authors： Katsafanas GC,Grem JL,Blough HA,Moss B

更新日期：1997-09-15 00:00:00

abstract：:The location of the RNA start point of in vivo Q-activated late gene RNA of bacteriophage lambda has been determined to be identical to the start point of in vitro 6 S RNA. The 6 S RNA is made early in infection and is efficiently antiterminated by Q. Two RNase III cut sites are located within Q-dependent RNA sequence...

journal_title：Virology

authors： Daniels DL,Subbarao MN,Blattner FR,Lozeron HA

更新日期：1988-12-01 00:00:00

abstract：:We constructed a sensitive and quantitative assay system to examine human T-cell leukemia virus type I (HTLV-I) envelope (env) glycoprotein-mediated cell fusion in which T7 RNA polymerase in donor cells coexpressing env glycoproteins activates a reporter gene in recipient cells upon cell fusion. An efficient expressio...

journal_title：Virology

authors： Okuma K,Nakamura M,Nakano S,Niho Y,Matsuura Y

更新日期：1999-02-15 00:00:00

abstract：:The epithelium of the cervix uteri has been reported to be frequently coinfected with both human papillomaviruses (HPV) and helper virus-dependent adenoassociated viruses (AAV). Seroepidemiological data suggest that AAV infection could inhibit cervical cancer that is caused by specific ("high-risk") types of papilloma...

journal_title：Virology

authors： Walz CM,Correa-Ochoa MM,Müller M,Schlehofer JR

更新日期：2002-02-01 00:00:00