Abstract:
:The roles of two porcine circovirus replication initiator proteins, Rep and Rep׳, in generating copy-release and rolling-circle DNA replication intermediates were determined. Rep uses the supercoiled closed-circular genome (ccc) to initiate leading-strand synthesis (identical to copy-release replication) and generates the single-stranded circular (ssc) genome from the displaced DNA strand. In the process, a minus-genome primer (MGP) necessary for complementary-strand synthesis, from ssc to ccc, is synthesized. Rep׳ cleaves the growing nascent-strand to regenerate the parent ccc molecule. In the process, a Rep׳-DNA hybrid containing the right palindromic sequence (at the origin of DNA replication) is generated. Analysis of the virus particle showed that it is composed of four components: ssc, MGP, capsid protein and a novel Rep-related protein (designated Protein-3).
journal_name
Virologyjournal_title
Virologyauthors
Cheung AKdoi
10.1016/j.virol.2015.01.004subject
Has Abstractpub_date
2015-07-01 00:00:00pages
43-50eissn
0042-6822issn
1096-0341pii
S0042-6822(15)00005-7journal_volume
481pub_type
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