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In vitro exposure system for study of aerosolized influenza virus.

Abstract：

:Infection of adherent cell monolayers using a liquid inoculum represents an established method to reliably and quantitatively study virus infection, but poorly recapitulates the exposure and infection of cells in the respiratory tract that occurs during infection with aerosolized pathogens. To better simulate natural infection in vitro, we adapted a system that generates viral aerosols similar to those exhaled by infected humans to the inoculation of epithelial cell monolayers. Procedures for cellular infection and calculation of exposure dose were developed and tested using viruses characterized by distinct transmission and pathogenicity phenotypes: an HPAI H5N1, an LPAI H7N9, and a seasonal H3N2 virus. While all three aerosolized viruses were highly infectious in a human bronchial epithelial cell line (Calu-3) cultured submerged in media, differences between the viruses were observed in primary human alveolar epithelial cells and in Calu-3 cells cultured at air-liquid interface. This system provides a novel enhancement to traditional in vitro experiments, particularly those focused on the early stages of infection.

journal_name

Virology

journal_title

Virology

authors

Creager HM,Zeng H,Pulit-Penaloza JA,Maines TR,Tumpey TM,Belser JA

doi

10.1016/j.virol.2016.10.007

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

62-70

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(16)30301-4

journal_volume

500

pub_type

杂志文章

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