当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Structural and functional analysis of the baculovirus single-stranded DNA-binding protein LEF-3.

Structural and functional analysis of the baculovirus single-stranded DNA-binding protein LEF-3.

Abstract：

:The single-stranded DNA-binding protein LEF-3 of Autographa californica multinucleocapsid nucleopolyhedrovirus consists of 385 amino acid residues, forms oligomers, and promotes Mg2+-independent unwinding of DNA duplexes and annealing of complementary DNA strands. Partial proteolysis revealed that the DNA-binding domain of LEF-3 is located within a central region (residues 28 to 326) that is relatively resistant to proteolysis. In contrast, the N-terminus (27 residues) and C-terminal portion (59 residues) are not involved in interaction with DNA and are readily accessible to proteolytic digestion. Circular dichroism analyses showed that LEF-3 is a folded protein with an estimated alpha-helix content of more than 40%, but it is structurally unstable and undergoes unfolding in aqueous solutions at temperatures near 50 degrees C. Unfolding eliminated the LEF-3 domains that are resistant to proteolysis and randomized the digestion pattern by trypsin. The structural transition was irreversible and was accompanied by the generation of high molecular weight (MW) complexes. The thermal treatment inhibited DNA-binding and unwinding activity of LEF-3 but markedly stimulated its annealing activity. We propose that the shift in LEF-3 activities resulted from the generation of the high MW protein complexes, that specifically stimulate the annealing of complementary DNA strands by providing multiple DNA-binding sites and bringing into close proximity the interacting strands. The unfolded LEF-3 was active in a strand exchange reaction suggesting that it could be involved in the production of recombination intermediates.

journal_name

Virology

journal_title

Virology

authors

Mikhailov VS,Okano K,Rohrmann GF

doi

10.1016/j.virol.2005.11.027

subject

Has Abstract

pub_date

2006-03-15 00:00:00

pages

469-78

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(05)00773-7

journal_volume

346

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • HPV8 activates cellular gene expression mainly through Sp1/3 binding sites.

    abstract：:The human papillomavirus type 8 (HPV8) is associated with skin cancer development. The goal of this study was to investigate the effects of HPV8 oncoproteins on cellular gene expression and the identification of key regulators. We performed affymetrix microarray analyses to identify differentially expressed genes and ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.06.019

    authors： Kirschberg M,Heuser S,Syed AS,Steger G,Majewski S,Hufbauer M,Akgül B

    更新日期：2019-09-01 00:00:00

  • Domain- and nucleotide-specific Rev response element regulation of feline immunodeficiency virus production.

    abstract：:Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.04.009

    authors： Na H,Huisman W,Ellestad KK,Phillips TR,Power C

    更新日期：2010-09-01 00:00:00

  • Mutations in the region encoding the central domain of helper component-proteinase (HC-Pro) eliminate potato virus X/potyviral synergism.

    abstract：:Coinfection of tobacco plants with potato virus X (PVX) and any of several members of the potyvirus group causes a synergistic disease characterized by a dramatic increase in symptom severity correlated with a 3- to 10-fold increase in the accumulation of PVX in the first systemically infected leaves. We have recently...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8488

    authors： Shi XM,Miller H,Verchot J,Carrington JC,Vance VB

    更新日期：1997-04-28 00:00:00

  • Recombinant measles viruses defective for RNA editing and V protein synthesis are viable in cultured cells.

    abstract：:The measles virus (MV) phosphoprotein (P) gene encodes three proteins, P, C, and V. The V protein is synthesized by pseudo-templated transcription, also designated as RNA editing: during P gene transcription one G residue is inserted at a defined position in about 50% of the mRNAs. To study the importance of sequence ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.8339

    authors： Schneider H,Kaelin K,Billeter MA

    更新日期：1997-01-20 00:00:00

  • ERV3, a full-length human endogenous provirus: chromosomal localization and evolutionary relationships.

    abstract：:A full-length human endogenous provirus termed ERV3 was isolated from a human fetal recombinant DNA library by low stringency hybridization with two probes: baboon endogenous virus LTR; and a pol-env subclone from the endogenous chimpanzee provirus, CH2. DNA sequencing within the clone and comparisons with other retro...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90347-7

    authors： O'Connell C,O'Brien S,Nash WG,Cohen M

    更新日期：1984-10-30 00:00:00

  • Identification of viral membrane proteins required for cell fusion and viral dissemination that are modified during vaccinia virus persistence.

    abstract：:Wild-type vaccinia virus WR strain forms non-fusogenic (F-) large plaques and is hemagglutinin positive (HA+) under normal conditions of virus infection. We have analyzed a collection of spontaneous, highly attenuated mutants of vaccinia virus isolated from persistently infected Friend erythroleukemia cells (E. Paez, ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1018

    authors： Ortiz MA,Paez E

    更新日期：1994-01-01 00:00:00

  • In vivo infection of ramified microglia from adult cat central nervous system by feline immunodeficiency virus.

    abstract：:Infection of microglial cells by the human immunodeficiency virus (HIV) is supposed to play an important role in the pathogenesis of AIDS-related central nervous system (CNS) complications. So far, however, experimental data about interactions between HIV and ramified microglia from the adult CNS were only occasionall...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0152

    authors： Hein A,Martin JP,Koehren F,Bingen A,Dörries R

    更新日期：2000-03-15 00:00:00

  • Inhibition of HIV-1 replication by cyclosporine A or related compounds correlates with the ability to disrupt the Gag-cyclophilin A interaction.

    abstract：:The HIV-1 Gag polyprotein specifically incorporates the cellular peptidylprolyl isomerase cyclophilin A into virions. HIV-1 replication is inhibited by cyclosporine A, an immunosuppressive drug which binds with high affinity to cyclophilin A and precludes interaction with the Gag polyprotein. Using a panel of four dru...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0421

    authors： Franke EK,Luban J

    更新日期：1996-08-01 00:00:00

  • Editing the human cytomegalovirus genome with the CRISPR/Cas9 system.

    abstract：:Human Cytomegalovirus (HCMV) is an opportunistic pathogen that causes substantial disease in neonates and immunocompromised individuals. Reverse genetic analysis of the HCMV genome is a powerful tool to dissect the roles that various viral genes play during infection. However, genetic engineering of HCMV is hampered b...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2019.01.021

    authors： King MW,Munger J

    更新日期：2019-03-01 00:00:00

  • Characterization of human papillomavirus type 6b DNA isolated from an invasive squamous carcinoma of the vulva.

    abstract：:Human papillomavirus type 6 (HPV 6) DNA was detected in an episomal form in DNA extracted from an invasive squamous carcinoma of the vulva. The viral DNA, designated HPV6-T70, was molecularly cloned. Restriction analysis of the HPV6-T70 genome revealed an insertion of approximately 35 bp in the 5' portion of the upstr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90676-9

    authors： Kasher MS,Roman A

    更新日期：1988-07-01 00:00:00

  • The metabolism of host RNAs in cells infected by an adenovirus E4 mutant.

    abstract：:Mutants of adenovirus type 5 (Ad5) that lack early region 4 (E4) are defective in the expression of viral late genes. E4 mutants exhibit dramatically reduced levels of both cytoplasmic and nuclear viral late RNAs compared to wild-type virus, due principally to reduced stability of unprocessed viral late RNA in the nuc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90498-z

    authors： Sandler AB,Ketner G

    更新日期：1991-03-01 00:00:00

  • Identification of tolerated insertion sites in poliovirus non-structural proteins.

    abstract：:Insertion of nucleotide sequences encoding "tags" that can be expressed in specific viral proteins during an infection is a useful strategy for purifying viral proteins and their functional complexes from infected cells and/or for visualizing the dynamics of their subcellular location over time. To identify regions in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.09.028

    authors： Teterina NL,Lauber C,Jensen KS,Levenson EA,Gorbalenya AE,Ehrenfeld E

    更新日期：2011-01-05 00:00:00

  • The genome of the archaeal virus SIRV1 has features in common with genomes of eukaryal viruses.

    abstract：:The virus SIRV1 of the extremely thermophilic archaeon Sulfolobus has a double-stranded DNA genome similar in architecture to the genomes of eukaryal viruses of the families Poxviridae, Pycodnaviridae, and Asfarviridae: the two strands of the 32,301 bp long linear genome are covalently connected forming a continuous p...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0776

    authors： Blum H,Zillig W,Mallok S,Domdey H,Prangishvili D

    更新日期：2001-03-01 00:00:00

  • Down-regulation of the internal ribosome entry site (IRES)-mediated translation of the hepatitis C virus: critical role of binding of the stem-loop IIId domain of IRES and the viral core protein.

    abstract：:In a previous study, we observed that hepatitis C virus (HCV) core protein specifically inhibits translation initiated by an HCV internal ribosome entry site (IRES). To investigate the mechanism by which down-regulation of HCV translation occurs, a series of mutations were introduced into the IRES element, as well as ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.10.013

    authors： Shimoike T,Koyama C,Murakami K,Suzuki R,Matsuura Y,Miyamura T,Suzuki T

    更新日期：2006-02-20 00:00:00

  • The termini of the chlorella virus PBCV-1 genome are identical 2.2-kbp inverted repeats.

    abstract：:The Chlorella virus PBCV-1 genome is a linear nonpermuted 333-kbp dsDNA molecule with covalently closed hairpin termini. The termini (minus the hairpin) are identical inverted repeats of at least 2185 bases after which the sequence diverges. The inverted repeats contain two small potential open reading frames and seve...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90089-t

    authors： Strasser P,Zhang YP,Rohozinski J,Van Etten JL

    更新日期：1991-02-01 00:00:00

  • The RING finger domain of the varicella-zoster virus open reading frame 61 protein is required for its transregulatory functions.

    abstract：:Varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein transactivates expression of VZV promoters. VZV ORF61 is functionally homologous to herpes simplex virus type 1 (HSV-1)-infected cell protein 0 (ICP0); however, amino acid sequence homology of these two proteins is limited to the RING finger domains, a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1639

    authors： Moriuchi H,Moriuchi M,Cohen JI

    更新日期：1994-11-15 00:00:00

  • Lassa-VSV chimeric virus targets and destroys human and mouse ovarian cancer by direct oncolytic action and by initiating an anti-tumor response.

    abstract：:Ovarian cancer is the third most common female cancer, with poor survival in later stages of metastatic spread. We test a chimeric virus consisting of genes from Lassa and vesicular stomatitis viruses, LASV-VSV; the native VSV glycoprotein is replaced by the Lassa glycoprotein, greatly reducing neurotropism. Human ova...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.10.009

    authors： van den Pol AN,Zhang X,Lima E,Pitruzzello M,Albayrak N,Alvero A,Davis JN,Mor G

    更新日期：2020-11-12 00:00:00

  • Recurrence of WHV integration in the b3n locus in woodchuck hepatocellular carcinoma.

    abstract：:Frequent occurrence of woodchuck hepatitis virus DNA (WHV DNA) integration into or in proximity to myc oncogenes and in the win locus of cellular genome in woodchuck hepatocellular carcinomas (HCC) has been described by several authors. We report a further cellular locus as a recurrent target for WHV integration in wo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.9936

    authors： Bruni R,Argentini C,D'Ugo E,Giuseppetti R,Ciccaglione AR,Rapicetta M

    更新日期：1995-12-01 00:00:00

  • Identification of the essential cysteine and histidine residues of the turnip yellow mosaic virus protease.

    abstract：:The nonstructural protein expressed from ORF-206 of turnip yellow mosaic virus is proteolytically processed to produce N-terminal 150-kDa and C-terminal 70-kDa proteins. Through the use of linker insertion and deletion analyses coupled to in vitro translation, we have delimited the protease domain to residues 731-885 ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1017

    authors： Bransom KL,Dreher TW

    更新日期：1994-01-01 00:00:00

  • Demonstration of the specificity of poliovirus encapsidation using a novel replicon which encodes enzymatically active firefly luciferase.

    abstract：:The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9046

    authors： Porter DC,Ansardi DC,Wang J,McPherson S,Moldoveanu Z,Morrow CD

    更新日期：1998-03-30 00:00:00

  • Shared ancestry of herpes simplex virus 1 strain Patton with recent clinical isolates from Asia and with strain KOS63.

    abstract：:Herpes simplex virus 1 (HSV-1) is a widespread pathogen that persists for life, replicating in surface tissues and establishing latency in peripheral ganglia. Increasingly, molecular studies of latency use cultured neuron models developed using recombinant viruses such as HSV-1 GFP-US11, a derivative of strain Patton ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.09.016

    authors： Pourchet A,Copin R,Mulvey MC,Shopsin B,Mohr I,Wilson AC

    更新日期：2017-12-01 00:00:00

  • Perturbation of differentiated functions during viral infection in vivo. II. Viral reassortants map growth hormone defect to the S RNA of the lymphocytic choriomeningitis virus genome.

    abstract：:Lymphocytic choriomeningitis virus (LCMV) Armstrong caused disordered growth and glucose metabolism secondary to growth hormone deficiency in infected C3H/St mice. In contrast, LCMV strain WE replicated in fewer growth hormone-producing cells, failed to disrupt growth hormone synthesis, and did not cause growth hormon...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90431-3

    authors： Riviere Y,Ahmed R,Southern P,Oldstone MB

    更新日期：1985-04-15 00:00:00

  • The V protein of the paramyxovirus SV5 interacts with damage-specific DNA binding protein.

    abstract：:The simian parainfluenza virus 5 (SV5) V/P gene encodes two proteins: V and the phosphoprotein P. The V and P proteins are amino coterminal for 164 residues, but they have unique carboxyl termini. The unique carboxyl terminus of V contains seven cysteine residues, resembles a zinc finger, and binds two atoms of zinc. ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9317

    authors： Lin GY,Paterson RG,Richardson CD,Lamb RA

    更新日期：1998-09-15 00:00:00

  • The mannosidase inhibitors 1-deoxymannojirimycin and swainsonine have no effect on the biosynthesis and infectivity of Rous sarcoma virus.

    abstract：:The effects of inhibitors, which interfere with oligosaccharide trimming by blocking mannosidases, on the processing and export of the viral glycoproteins of Rous sarcoma virus (RSV), have been studied. 1-Deoxymannojirimycin (DIM) prevents removal of mannose residues from the Man9 (GlcNAc)2 oligosaccharide whereas swa...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90122-9

    authors： Bosch JV,Tlusty A,McDowell W,Legler G,Schwarz RT

    更新日期：1985-05-01 00:00:00

  • Next generation sequencing of DNA-launched Chikungunya vaccine virus.

    abstract：:Chikungunya virus (CHIKV) represents a pandemic threat with no approved vaccine available. Recently, we described a novel vaccination strategy based on iDNA® infectious clone designed to launch a live-attenuated CHIKV vaccine from plasmid DNA in vitro or in vivo. As a proof of concept, we prepared iDNA plasmid pCHIKV-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.01.009

    authors： Hidajat R,Nickols B,Forrester N,Tretyakova I,Weaver S,Pushko P

    更新日期：2016-03-01 00:00:00

  • Poliovirus replication and spread in primary neuron cultures.

    abstract：:While some neurotropic viruses cause rapid central nervous system (CNS) disease upon entry into the brain parenchyma, other viruses that are cytolytic in the periphery either result in little neuropathology or are associated with a protracted course of CNS disease consistent with persistent infection. One such virus, ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.05.032

    authors： Daley JK,Gechman LA,Skipworth J,Rall GF

    更新日期：2005-09-15 00:00:00

  • Host cell p53 associates with the feline calicivirus major viral capsid protein VP1, the protease-polymerase NS6/7, and the double-stranded RNA playing a role in virus replication.

    abstract：:p53 is implicated in several cellular pathways such as induction of cell-cycle arrest, differentiation, senescence, and apoptosis. p53 is activated by a broad range of stress signals, including viral infections. While some viruses activate p53, others induce its inactivation, and occasionally p53 is differentially mod...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2020.08.008

    authors： Trujillo-Uscanga A,Gutiérrez-Escolano AL

    更新日期：2020-11-01 00:00:00

  • Formation of surface tubules and fluorescent foci in Arabidopsis thaliana protoplasts expressing a fusion between the green fluorescent protein and the cauliflower mosaic virus movement protein.

    abstract：:The movement protein (MP) of cauliflower mosaic virus (CaMV) is a multifunctional protein that potentiates the cell-to-cell and long distance movement of the virus. Functional domains in the CaMV MP were determined by analyzing deletions in green fluorescent protein (GFP)-MP fusions transfected into Arabidopsis thalia...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0292

    authors： Huang Z,Han Y,Howell SH

    更新日期：2000-05-25 00:00:00

  • Differential virus restriction patterns of rhesus macaque and human APOBEC3A: implications for lentivirus evolution.

    abstract：:The human apolipoprotein B mRNA editing enzyme catalytic peptide-like 3 (APOBEC3; A3) family of proteins (A3A-H) are known to restrict various retroviruses and retroelements, but the full complement of rhesus macaque A3 proteins remains unclear. We report the isolation and characterization of the hA3A homologue from r...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.07.017

    authors： Schmitt K,Guo K,Algaier M,Ruiz A,Cheng F,Qiu J,Wissing S,Santiago ML,Stephens EB

    更新日期：2011-10-10 00:00:00

  • Inclusion of the NS2-specific exon in minute virus of mice mRNA is facilitated by an intronic splicing enhancer that affects definition of the downstream small intron.

    abstract：:Alternative splicing of pre-mRNAs plays a critical role in maximizing the coding capacity of the small parvovirus genome. The small-intron region of minute virus of mice (MVM) pre-mRNAs undergoes an unusual pattern of overlapping alternative splicing, using two donors, D1 and D2, and two acceptors, A1 and A2, within a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9696

    authors： Haut DD,Pintel DJ

    更新日期：1999-05-25 00:00:00