Mutations in the region encoding the central domain of helper component-proteinase (HC-Pro) eliminate potato virus X/potyviral synergism.

abstract：:Five regions on the coat protein of BNYVV which had been shown previously to be involved in the formation of continuous epitopes were further analyzed by means of synthetic overlapping peptides. It was found that at least some of these regions may encompass several overlapping epitopes (or parts thereof). Four monoclo...

journal_title：Virology

authors： Commandeur U,Koenig R,Manteuffel R,Torrance L,Lüddecke P,Frank R

更新日期：1994-01-01 00:00:00

abstract：:Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNA...

journal_title：Virology

authors： Scholthof KB,Scholthof HB,Jackson AO

更新日期：1995-08-01 00:00:00

abstract：:The phosphatidylinositol-3-kinase (PI3K)-Akt signaling pathway regulates several key cellular functions including protein synthesis, cell growth, glucose metabolism, and inflammation. Many viruses have evolved mechanisms to manipulate this signaling pathway to ensure successful virus replication. The human herpesvirus...

journal_title：Virology

authors： Liu X,Cohen JI

更新日期：2015-05-01 00:00:00

abstract：:The relationship between the cell cycle and the presence of the nuclear T antigen in simian virus 40-transformed rat cells independently derived from FR 3T3 cells has been investigated using a fluorescence-activated cell sorter and a combination of stainings for cellular DNA (4',6-diamidine-2-phenylindole dihydrochlor...

journal_title：Virology

authors： Imbert J,Lawrence JJ,Birg F

更新日期：1983-04-30 00:00:00

abstract：:The bipartite geminiviruses bean golden mosaic virus (BGMV), cabbage leaf curl virus (CabLCV), and tomato golden mosaic virus (TGMV) exhibit differential tissue tropism in Nicotiana benthamiana. In systemically infected leaves, BGMV remains largely confined to vascular-associated cells (phloem-limited), whereas CabLCV...

journal_title：Virology

authors： Qin Y,Petty IT

更新日期：2001-12-20 00:00:00

abstract：:We have investigated a novel strategy for coexpressing two genes from a retroviral vector. The 5' nontranslated leader region of at least some picornavirus RNAs contains a sequence that can act as an internal ribosome entry site allowing initiation of translation at a downstream AUG codon in a 5' cap-independent manne...

journal_title：Virology

authors： Koo HM,Brown AM,Kaufman RJ,Prorock CM,Ron Y,Dougherty JP

更新日期：1992-02-01 00:00:00

abstract：:Although dengue (DEN) virus is the etiologic agent of dengue fever, the most prevalent vector-borne viral disease in the world, precise information on the antigenic structure of the dengue virion is limited. We have prepared a set of murine monoclonal antibodies (MAbs) specific for the envelope (E) glycoprotein of DEN...

journal_title：Virology

authors： Roehrig JT,Bolin RA,Kelly RG

更新日期：1998-07-05 00:00:00

abstract：:Little is known about antigen-specific T-cell responses to viruses in teleosts due to a lack of a suitable experimental system using inbred or clonal animals. In the present study we have successfully induced an in vitro generation of virus-specific cytotoxic T-cells (CTLs) from isogeneic ginbuna crucian carp. Respond...

journal_title：Virology

authors： Somamoto T,Okamoto N,Nakanishi T,Ototake M,Nakao M

更新日期：2009-06-20 00:00:00

abstract：:Hypervariable region 1 (HVR1) proteins of hepatitis C virus (HCV) have been reported to react broadly with sera of patients with HCV infection. However, the variability of the broad reactivity of individual HVR1 proteins has not been elucidated. We assessed the reactivity of 25 different HVR1 proteins (genotype 1b) wi...

journal_title：Virology

authors： Watanabe K,Yoshioka K,Ito H,Ishigami M,Takagi K,Utsunomiya S,Kobayashi M,Kishimoto H,Yano M,Kakumu S

更新日期：1999-11-10 00:00:00

abstract：:Alphaherpesviruses comprise closely related viruses of man and animal, including herpes simplex virus, varicella-zoster virus and pseudorabies virus (PRV). Here, using methyl-beta-cyclodextrin and fluorescently tagged PRV, we directly show that depletion of cholesterol from the plasma membrane of host cells significan...

journal_title：Virology

authors： Desplanques AS,Nauwynck HJ,Vercauteren D,Geens T,Favoreel HW

更新日期：2008-07-05 00:00:00

abstract：:The immediate-early transcripts of pseudorabies virus have been located in a region of the genome situated internally within the inverted repeat between map positions 0.99 and 0.95. A single immediate-early transcript (approximately 6 kb) can be detected both in the cytoplasmic and nuclear fractions of infected, cyclo...

journal_title：Virology

authors： Ihara S,Feldman L,Watanabe S,Ben-Porat T

更新日期：1983-12-01 00:00:00

abstract：:In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified...

journal_title：Virology

authors： Duarte M,Tobler K,Bridgen A,Rasschaert D,Ackermann M,Laude H

更新日期：1994-02-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) is an economically important cause of post-weaning multisystemic wasting syndrome (PMWS) in weanling piglets. Current commercial vaccines against PCV2 are highly effective. Yet, a recurring emergence of new genotypes in vaccinated herds necessitates a better understanding of protective...

journal_title：Virology

authors： Ilha M,Nara P,Ramamoorthy S

更新日期：2020-01-15 00:00:00

abstract：:The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6-methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA ...

journal_title：Virology

authors： Zhang Y,Nelson M,Nietfeldt J,Xia Y,Burbank D,Ropp S,Van Etten JL

更新日期：1998-01-20 00:00:00

abstract：:Pea enation mosaic virus (PEMV) RNA was isolated from virions and then translated in rabbit reticulocyte lysates and in wheat germ extracts. RNA 1 (apparent molecular weight, Mr 1.77 x 106) was shown to code for two major translation products, vp2 (Mr 88,000) and vp4 (Mr 36,000) plus a high-molecular-weight minor prod...

journal_title：Virology

authors： Gabriel CJ,de Zoeten GA

更新日期：1984-12-01 00:00:00

abstract：:In response to oncogenic insults, normal human cells execute a defense response that culminates in cellular suicide, apoptosis. Normal human diploid fibroblasts expressing the human papillomavirus type 16 (HPV-16) E7 oncoprotein are predisposed to apoptosis when they are deprived of growth factors. Even though a domin...

journal_title：Virology

authors： Eichten A,Rud DS,Grace M,Piboonniyom SO,Zacny V,Münger K

更新日期：2004-02-05 00:00:00

abstract：:Wasting is a sign of various underlying disorders and is a common feature of cancer, sepsis, and AIDS. We have developed an in vivo model to study the various stages of wasting following infection of mice with lymphocytic choriomeningitis virus cl-13. Using this model we have identified four distinct stages of wasting...

journal_title：Virology

authors： Stamm A,Valentine L,Potts R,Premenko-Lanier M

更新日期：2012-04-10 00:00:00

abstract：:Productive infection by human immunodeficiency virus type I (HIV-1) in the central nervous system (CNS) involves mainly macrophages and microglial cells. A frequency of less than 10% of human astrocytes is estimated to be infectable with HIV-1. Nonetheless, this relatively low percentage of infected astrocytes, but as...

journal_title：Virology

authors： Fang J,Acheampong E,Dave R,Wang F,Mukhtar M,Pomerantz RJ

更新日期：2005-06-05 00:00:00

abstract：:Disruption of the perforin gene results in primary immunodeficiency and an increased susceptibility to opportunistic pathogens. Perforin-deficient (PKO) mice fail to clear primary lymphocytic choriomeningitis virus (LCMV) Armstrong, resulting in persistent infection and functional exhaustion of virus-specific CD8+ T c...

journal_title：Virology

authors： Humphreys TD,Khanolkar A,Badovinac VP,Harty JT

更新日期：2008-01-20 00:00:00

abstract：:An infectious and full-length molecular clone of genomic human spumaretrovirus (HSRV) DNA was constructed. The infectivity of the pHSRV13 clone was demonstrated after transfection into susceptible cells by passage of HSRV-specific cytopathic effects as a cell-free culture supernatant, by electron microscopy of HSRV pa...

journal_title：Virology

authors： Löchelt M,Zentgraf H,Flügel RM

更新日期：1991-09-01 00:00:00

abstract：:A nonhemagglutinating mutant of a 1978 isolate of canine parvovirus (CPV) was derived after repeated passages in the NLFK feline kidney cell line. The mutant CPV was antigenically indistinguishable from wild-type virus when tested with 82 monoclonal antibodies, and it replicated in cat and dog cell lines in culture. S...

journal_title：Virology

authors： Parrish CR,Burtonboy G,Carmichael LE

更新日期：1988-03-01 00:00:00

abstract：:Studies were undertaken to compare the effect of poly(A)s from various sources on selective inhibition of protein synthesis in the reticulocyte lysate system programmed with viral and cellular mRNAs. RNA synthesized in vitro by vaccinia virus cores in the presence of only ATP inhibited overall HeLa cell polypeptide sy...

journal_title：Virology

authors： Bablanian R,Goswami SK,Esteban M,Banerjee AK

更新日期：1987-12-01 00:00:00

abstract：:The behavior of two genetically different molecular clones of visna virus KV1772-kv72/67 and LV1-1KS1 was compared in vivo and in vitro. On intracerebral inoculation, clone KV1772-kv72/67 induced a similar response in five sheep as has already been reported with neurovirulent derivates of visna virus. Virus was freque...

journal_title：Virology

authors： Torsteinsdóttir S,Agnarsdóttir G,Matthíasdóttir S,Rafnar B,Andrésdóttir V,Andrésson OS,Staskus K,Pétursson G,Pálsson PA,Georgsson G

更新日期：1997-03-17 00:00:00

abstract：:An SH3-binding domain within the Nef protein of primate lentiviruses has been reported to be important to viral replication and infectivity and dispensable for CD4 downregulation, but its precise role remains unclear. This study investigates the effects of mutations in both the polyproline helix and in the hydrophobic...

journal_title：Virology

authors： Craig HM,Pandori MW,Riggs NL,Richman DD,Guatelli JC

更新日期：1999-09-15 00:00:00

abstract：:Multiple RNA species which hybridize to a cloned cDNA probe of the turnip crinkle virus satellite RNA (S-TCV) are found both in virions and in plants infected with virus particles containing S-TCV. At least six of these RNAs appear to be multimeric forms of the satellite RNA based on their migration in denaturing gels...

journal_title：Virology

authors： Altenbach SB,Howell SH

更新日期：1984-04-15 00:00:00

abstract：:Putative replication complexes of cauliflower mosaic virus (CaMV) which resemble hepatitis B virus replication complexes in several respects have been identified. The CaMV replication complexes contain a heterogeneous population of minus-strand DNA ranging in size from less than 600 nucleotides to full genome length o...

journal_title：Virology

authors： Marsh L,Kuzj A,Guilfoyle T

更新日期：1985-05-01 00:00:00

abstract：:A new set of lambdoid phages (mEp) classified into different immunity groups was previously described. Phages mEp213, mEp237, and mEp410 were unable to grow in mEp167 lysogenic cells, presumably due to an exclusion mechanism expressed constitutively by the mEp167 repressed prophage. In this work, to analyze the exclus...

journal_title：Virology

authors： Uc-Mass A,Loeza EJ,de la Garza M,Guarneros G,Hernández-Sánchez J,Kameyama L

更新日期：2004-11-24 00:00:00

abstract：:In this paper we present a technique developed to physically locate the HSV-1 gene(s) which restore neurovirulence to a non-neurovirulent HSV intertypic recombinant described in the preceding report. In brief, tissue culture cells are co-transfected with unit length RE6 DNA and restriction endonuclease fragmented HSV-...

journal_title：Virology

authors： Thompson RL,Wagner EK,Stevens JG

更新日期：1983-11-01 00:00:00

abstract：:Old world hantaviruses, causing hemorrhagic fever with renal syndrome (HFRS), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. T...

journal_title：Virology

authors： Koch J,Liang M,Queitsch I,Kraus AA,Bautz EK

更新日期：2003-03-30 00:00:00

abstract：:We investigated the localization of host mRNA during Rift Valley fever virus (RVFV) infection. Fluorescence in situ hybridization revealed that infection with RVFV altered the localization of host mRNA. mRNA accumulated in the nuclei of RVFV-infected but not mock-infected cells. Further, overexpression of the NSS gene...

journal_title：Virology

authors： Copeland AM,Van Deusen NM,Schmaljohn CS

更新日期：2015-12-01 00:00:00