Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica.


:Although dengue (DEN) virus is the etiologic agent of dengue fever, the most prevalent vector-borne viral disease in the world, precise information on the antigenic structure of the dengue virion is limited. We have prepared a set of murine monoclonal antibodies (MAbs) specific for the envelope (E) glycoprotein of DEN 2 virus and used these antibodies in a comprehensive biological and biochemical analysis to identify 16 epitopes. Following domain nomenclature developed for the related flavivirus, tick-borne encephalitis, three functional domains were identified. Five epitopes associated with domain A were arranged in three spatially independent regions. These A-domain epitopes were destroyed by reduction, and antibodies reactive with these epitopes were able to block virus hemagglutination, neutralize virus infectivity, and block virus-mediated cell membrane fusion. Domain-A epitopes were present on the full-length E glycoprotein, a 45-kDa tryptic peptide representing its first 400 amino acids (aa) and a 22-kDa tryptic peptide representing at least aa 1-120. Four epitopes mapped into domain B, as determined by their partial resistance to reduction and the localization of these epitopes on a 9-kDa tryptic or chymotryptic peptide fragment (aa 300-400). One domain-B-reactive MAb was also capable of binding to a DEN 2 synthetic peptide corresponding to aa 333-351 of the E glycoprotein, confirming the location of this domain. Domain-B epitopes elicited MAbs that were potent neutralizers of virus infectivity and blocked hemagglutination, but they did not block virus-mediated cell-membrane fusion. Domains A and B were spatially associated. As with tick-borne encephalitis virus, determination of domain C was more problematic; however, at least four epitopes had biochemical characteristics consistent with C-domain epitopes.






Roehrig JT,Bolin RA,Kelly RG




Has Abstract


1998-07-05 00:00:00
















  • Sequences of the N and M genes of the sigma virus of Drosophila and evolutionary comparison.

    abstract::The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa,...


    pub_type: 杂志文章


    authors: Bras F,Teninges D,Dezelee S

    更新日期:1994-04-01 00:00:00

  • HPV8 activates cellular gene expression mainly through Sp1/3 binding sites.

    abstract::The human papillomavirus type 8 (HPV8) is associated with skin cancer development. The goal of this study was to investigate the effects of HPV8 oncoproteins on cellular gene expression and the identification of key regulators. We performed affymetrix microarray analyses to identify differentially expressed genes and ...


    pub_type: 杂志文章


    authors: Kirschberg M,Heuser S,Syed AS,Steger G,Majewski S,Hufbauer M,Akgül B

    更新日期:2019-09-01 00:00:00

  • Cloning of SV40 genomes from human brain tumors.

    abstract::From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...


    pub_type: 杂志文章


    authors: Krieg P,Scherer G

    更新日期:1984-10-30 00:00:00

  • Substrate specificity of the NS3 serine proteinase of hepatitis C virus as determined by mutagenesis at the NS3/NS4A junction.

    abstract::Hepatitis C virus (HCV) encodes a polyprotein that is processed to produce the structural and nonstructural proteins of the virus. Nonstructural protein 3 (NS3) is a serine proteinase that cleaves the polyprotein to release the NS4A, NS4B, NS5A, and NS5B proteins. To characterize the substrate specificity of NS3, we s...


    pub_type: 杂志文章


    authors: Leinbach SS,Bhat RA,Xia SM,Hum WT,Stauffer B,Davis AR,Hung PP,Mizutani S

    更新日期:1994-10-01 00:00:00

  • Complementation analysis of the dales collection of vaccinia virus temperature-sensitive mutants.

    abstract::A collection of randomly generated temperature-sensitive (ts) vaccinia virus (strain IHD-W) mutants were reported by S. Dales et al., (1978, Virology, 84, 403-428) in 1978 and characterized by electron microscopy. We have performed further genetic analysis on the Dales collection of mutants to make the mutants more us...


    pub_type: 杂志文章


    authors: Lackner CA,D'Costa SM,Buck C,Condit RC

    更新日期:2003-01-20 00:00:00

  • Analysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus.

    abstract::The complete nucleotide sequence of the nucleocapsid (N) gene of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus of plant rhabdoviruses, has been determined from cDNA clones of both the viral genomic and messenger RNAs. The previously identified N gene was adjacent to the 3' leader ...


    pub_type: 杂志文章


    authors: Wetzel T,Dietzgen RG,Geering AD,Dale JL

    更新日期:1994-08-01 00:00:00

  • Vesicular stomatitis virus and its defective interfering particles exhibit in vitro transcriptional and replicative competition for purified L-NS polymerase molecules.

    abstract::We have quantitated replication of the RNA genomes of vesicular stomatitis virus (VSV) and its defective interfering (DI) particles in a BHK21 cell-free system into which nucleocapsids were introduced in varying amounts and ratios, with or without addition of purified virus polymerase components. The quantitative tran...


    pub_type: 杂志文章


    authors: Giachetti C,Holland JJ

    更新日期:1989-05-01 00:00:00

  • Bombyx mori nucleopolyhedrovirus BM5 protein regulates progeny virus production and viral gene expression.

    abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mo...


    pub_type: 杂志文章


    authors: Kokusho R,Koh Y,Fujimoto M,Shimada T,Katsuma S

    更新日期:2016-11-01 00:00:00

  • Role of capsid sequence and immature nucleocapsid proteins p9 and p15 in Human Immunodeficiency Virus type 1 genomic RNA dimerization.

    abstract::HIV-1 genomic RNA (gRNA) dimerization is important for viral infectivity and is regulated by proteolytic processing of the Gag precursor protein (Pr55gag) under the direction of the viral protease. The processing occurs in successive steps and, to date, the step associated with formation of a wild-type (WT) level of g...


    pub_type: 杂志文章


    authors: Kafaie J,Dolatshahi M,Ajamian L,Song R,Mouland AJ,Rouiller I,Laughrea M

    更新日期:2009-03-01 00:00:00

  • MMTV accessory factor Naf affects cellular gene expression.

    abstract::Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, lu...


    pub_type: 杂志文章


    authors: Metzner C,Salmons B,Gunzburg WH,Gemeiner M,Miller I,Gesslbauer B,Kungl A,Dangerfield JA

    更新日期:2006-03-01 00:00:00

  • The hypervariable region 1 protein of hepatitis C virus broadly reactive with sera of patients with chronic hepatitis C has a similar amino acid sequence with the consensus sequence.

    abstract::Hypervariable region 1 (HVR1) proteins of hepatitis C virus (HCV) have been reported to react broadly with sera of patients with HCV infection. However, the variability of the broad reactivity of individual HVR1 proteins has not been elucidated. We assessed the reactivity of 25 different HVR1 proteins (genotype 1b) wi...


    pub_type: 杂志文章


    authors: Watanabe K,Yoshioka K,Ito H,Ishigami M,Takagi K,Utsunomiya S,Kobayashi M,Kishimoto H,Yano M,Kakumu S

    更新日期:1999-11-10 00:00:00

  • Apoptosis correlates with immune activation in intestinal lymphoid tissue from macaques acutely infected by a highly enteropathic simian immunodeficiency virus, SIVsmmPBj14.

    abstract::The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, induces an acutely lethal disease in experimentally inoculated pigtailed macaques, that is characterized by severe enteropathy and extensive immune activation, particularly within gut-associated lymphoid tissue (GALT). Experiments were conducted to deter...


    pub_type: 杂志文章


    authors: Gummuluru S,Novembre FJ,Lewis M,Gelbard HA,Dewhurst S

    更新日期:1996-11-01 00:00:00

  • Recovery of structurally intact and infectious poliovirus type 1 from HeLa cells during receptor-mediated endocytosis.

    abstract::Poliovirus type 1 enters HeLa cells by receptor-mediated endocytosis as an intact virus. Up to 30 min after adsorption complete virus particles still containing VP4 and sedimenting with 156 S could be recovered from the cells. These virus particles were N-antigenic and infectious. Thirty minutes after adsorption the r...


    pub_type: 杂志文章


    authors: Willingmann P,Barnert H,Zeichhardt H,Habermehl KO

    更新日期:1989-02-01 00:00:00

  • Low pH-dependent Sindbis virus-induced fusion of BHK cells: differences between strains correlate with amino acid changes in the E1 glycoprotein.

    abstract::Expression of alphavirus glycoproteins on the surface of infected cells leads to cell fusion after exposure to acidic pH. Two strains of Sindbis virus, AR339 (SV) and neuroadapted Sindbis virus (NSV), which differ in virulence for weanling mice, were found to differ in pH-dependent fusion. BHK-21 cells infected with S...


    pub_type: 杂志文章


    authors: Boggs WM,Hahn CS,Strauss EG,Strauss JH,Griffin DE

    更新日期:1989-04-01 00:00:00

  • Identification and characterization of the repetitive DNA element in the genome of insect iridescent virus type 6.

    abstract::The genome of the Chilo iridescent virus (CIV) was analyzed for existence of repetitive DNA sequences by DNA-DNA hybridization using a defined and complete gene library of the viral genome (209 kbp) and by heteroduplex mapping. These experiments revealed the presence of repetitive DNA elements in the CIV genome, which...


    pub_type: 杂志文章


    authors: Fischer M,Schnitzler P,Delius H,Darai G

    更新日期:1988-12-01 00:00:00

  • Equine herpesvirus 1 (EHV-1) glycoprotein M: effect of deletions of transmembrane domains.

    abstract::Equine herpesvirus 1 (EHV-1) recombinants that carry either a deletion of glycoprotein M (gM) or express mutant forms of gM were constructed. The recombinants were derived from strain Kentucky A (KyA), which also lacks genes encoding gE and gI. Plaques on RK13 cells induced by the gM-negative KyA were reduced in size ...


    pub_type: 杂志文章


    authors: Seyboldt C,Granzow H,Osterrieder N

    更新日期:2000-12-20 00:00:00

  • Persistent coinfection of T lymphocytes with HTLV-II and HIV and the role of syncytium formation in HIV-induced cytopathic effect.

    abstract::We previously demonstrated a high permissiveness of HTLV-II-transformed T lymphocytes (C3) to human immunodeficiency virus (HIV) infection in vitro, and that this infection results in the lysis of cells (D.C. Montefiori and W.M. Mitchell (1986) Virology 155, 726-731). We now show that a small percentage of HIV-infecte...


    pub_type: 杂志文章


    authors: Montefiori DC,Mitchell WM

    更新日期:1987-10-01 00:00:00

  • Complementation of deletion of the vaccinia virus E3L gene by the Escherichia coli RNase III gene.

    abstract::This work investigated whether the Escherichia coli RNase III gene, rnc+, could complement vp1080, a mutant vaccinia virus that is deleted of its E3L gene. Like E3L, rnc+ codes for a dsRNA binding protein that contains an additional nucleolytic activity. Rnc genes were cloned into the eukaryotic expression vector pMTV...


    pub_type: 杂志文章


    authors: Shors T,Jacobs BL

    更新日期:1997-01-06 00:00:00

  • Molecular characterization of human immunodeficiency virus (HIV)-1 and -2 in individuals from guinea-bissau with single or dual infections: predominance of a distinct HIV-1 subtype A/G recombinant in West Africa.

    abstract::Guinea-Bissau in West Africa has the highest prevalence of human immunodeficiency virus (HIV)-2 infection in the world, but recently the HIV-1 prevalence increased rapidly with the subsequent appearance of HIV-1 and HIV-2 dual infections. Information about the genetic subtypes of HIV in the region is limited. Therefor...


    pub_type: 杂志文章


    authors: Andersson S,Norrgren H,Dias F,Biberfeld G,Albert J

    更新日期:1999-09-30 00:00:00

  • Recombinant measles viruses defective for RNA editing and V protein synthesis are viable in cultured cells.

    abstract::The measles virus (MV) phosphoprotein (P) gene encodes three proteins, P, C, and V. The V protein is synthesized by pseudo-templated transcription, also designated as RNA editing: during P gene transcription one G residue is inserted at a defined position in about 50% of the mRNAs. To study the importance of sequence ...


    pub_type: 杂志文章


    authors: Schneider H,Kaelin K,Billeter MA

    更新日期:1997-01-20 00:00:00

  • Two regions of simian virus 40 large T-antigen independently extend the life span of primary C57BL/6 mouse embryo fibroblasts and cooperate in immortalization.

    abstract::Expression of the SV40 large T-antigen allows primary cells to escape senescence and thereby become immortalized. Immortalization occurs in two steps, extension of life span and acquisition of unlimited cell division potential. By following the increase in expression of a senescence-associated marker with increased ce...


    pub_type: 杂志文章


    authors: Tevethia MJ,Lacko HA,Conn A

    更新日期:1998-04-10 00:00:00

  • Gene flow and competitive exclusion of avian influenza A virus in natural reservoir hosts.

    abstract::Geographical separation of host species has shaped the avian influenza A virus gene pool into independently evolving Eurasian and American lineages, although phylogenetic evidence for gene flow and reassortment indicates that these lineages also mix on occasion. While the evolutionary dynamics of the avian influenza g...


    pub_type: 杂志文章


    authors: Bahl J,Vijaykrishna D,Holmes EC,Smith GJ,Guan Y

    更新日期:2009-08-01 00:00:00

  • HF1 and HF2: novel bacteriophages of halophilic archaea.

    abstract::Two novel halophilic archaebacterial bacteriophages, HF1 and HF2, were isolated from an Australian solar saltern. They were morphologically identical with icosahedral-shaped heads (diameter 58 nm) and contractile tails (length 94 nm). Other similarities included sensitivity to reduced ionic conditions, similar protein...


    pub_type: 杂志文章


    authors: Nuttall SD,Dyall-Smith ML

    更新日期:1993-12-01 00:00:00

  • Gene transfer into hematopoietic progenitor cells mediated by an adeno-associated virus vector.

    abstract::We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formati...


    pub_type: 杂志文章


    authors: LaFace D,Hermonat P,Wakeland E,Peck A

    更新日期:1988-02-01 00:00:00

  • The HBV-producing cell line HepG2-4A5: a new in vitro system for studying the regulation of HBV replication and for screening anti-hepatitis B virus drugs.

    abstract::All tissue culture systems for propagating HBV employed so far make use of tandemly arranged HBV genomes usually under the control of strong foreign promoters. Thus these systems are helpful for virus production but are of limited value in the investigation of the regulation of HBV replication or of the extent to whic...


    pub_type: 杂志文章


    authors: Weiss L,Kekulè AS,Jakubowski U,Bürgelt E,Hofschneider PH

    更新日期:1996-02-01 00:00:00

  • In vitro excision and replication of 5' telomeres of minute virus of mice DNA from cloned palindromic concatemer junctions.

    abstract::HeLa cell extracts containing wild-type copies of the minute virus of mice NS-1 polypeptide produced from a recombinant vaccinia virus vector could support the excision and replication of viral 5' telomeres from cloned concatemer junction fragments. Resolution did not occur if wild-type NS-1 was omitted from the extra...


    pub_type: 杂志文章


    authors: Cotmore SF,Nuesch JP,Tattersall P

    更新日期:1992-09-01 00:00:00

  • Antibodies targeting dengue virus envelope domain III are not required for serotype-specific protection or prevention of enhancement in vivo.

    abstract::The envelope (E) protein of dengue virus (DENV) is composed of three domains (EDI, EDII, EDIII) and is the main target of neutralizing antibodies. Many monoclonal antibodies that bind EDIII strongly neutralize DENV. However in vitro studies indicate that anti-EDIII antibodies contribute little to the neutralizing pote...


    pub_type: 杂志文章


    authors: Williams KL,Wahala WM,Orozco S,de Silva AM,Harris E

    更新日期:2012-07-20 00:00:00

  • Immunoprotection against influenza H5N1 virus by oral administration of enteric-coated recombinant Lactococcus lactis mini-capsules.

    abstract::Edible vaccines that can be made widely available and easily administered could bring great benefit to the worldwide battle against pandemic viral infections. They can be used not only for the vaccination of humans and domesticated animals, but also for wild herds and live stock which are otherwise difficult to vaccin...


    pub_type: 杂志文章


    authors: Lei H,Xu Y,Chen J,Wei X,Lam DM

    更新日期:2010-11-25 00:00:00

  • Evolution of poliovirus type I during 5.5 years of prolonged enteral replication in an immunodeficient patient.

    abstract::Poliovirus type 1 was isolated from an immunodeficient patient 4 days after onset of paresis (IS1) and after 5.5 years of prolonged enteral virus replication (IS2). Antigenic characterization revealed that IS1 was Sabin 1-like, whereas IS2 reacted like poliovirus 1 Mahoney. Complete genomic sequencing demonstrated the...


    pub_type: 杂志文章


    authors: Bellmunt A,May G,Zell R,Pring-Akerblom P,Verhagen W,Heim A

    更新日期:1999-12-20 00:00:00

  • DNA repair gene expression is increased in HPV positive head and neck squamous cell carcinomas.

    abstract::The incidence of head and neck squamous cell carcinomas (HNSCCs) is rising in developed countries. This is driven by an increase in HNSCCs caused by high-risk human papillomavirus (HPV) infections or HPV + HNSCCs. Compared to HNSCCs not caused by HPV (HPV- HNSCCs), HPV + HNSCCs are more responsive to therapy and assoc...


    pub_type: 杂志文章


    authors: Holcomb AJ,Brown L,Tawfik O,Madan R,Shnayder Y,Thomas SM,Wallace NA

    更新日期:2020-09-01 00:00:00