Abstract:
:The movement protein (MP) of cauliflower mosaic virus (CaMV) is a multifunctional protein that potentiates the cell-to-cell and long distance movement of the virus. Functional domains in the CaMV MP were determined by analyzing deletions in green fluorescent protein (GFP)-MP fusions transfected into Arabidopsis thaliana leaf protoplasts. GFP-MP accumulated at fluorescent foci at the cell periphery and in tubular structures extending from the protoplast surface. A region located near the center of MP was required for targeting GFP-MP to foci, whereas a larger region extending nearly to the N-terminus was needed for tubule formation. Cytoskeletal assembly inhibitors did not disrupt tubule formation or the accumulation of GFP-MP at foci, but brefeldin A, which disrupts the plant cell endomembrane system, did interfere with the formation of tubules but not foci.
journal_name
Virologyjournal_title
Virologyauthors
Huang Z,Han Y,Howell SHdoi
10.1006/viro.2000.0292subject
Has Abstractpub_date
2000-05-25 00:00:00pages
58-64issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(00)90292-7journal_volume
271pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Human herpesvirus-8 (HHV-8) variants have been found heterogeneously distributed among human populations living in diverse geographic regions, but their differential pathogenicity in Kaposi's sarcoma development remains controversial. In the present study, HHV-8 variant distribution has been analyzed in classic, iatro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.005
更新日期:2010-03-15 00:00:00
abstract::The host innate response to viral infection includes the production of interferons, which is dependent on the coordinated activity of multiple transcription factors. Herpes simplex virus 1 (HSV-1) has been shown to block efficient interferon expression by multiple mechanisms. We and others have demonstrated that HSV-1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.028
更新日期:2007-04-10 00:00:00
abstract::GV1B is one of five viroids that have recently been purified from grapevines. GV1B has now been sequenced and its 363 nucleotide residues can potentially form the typical rod-like structure of viroids with 67% of nucleotides base-paired. GV1B has highest sequence similarity with grapevine yellow speckle viroid (GYSV; ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90450-9
更新日期:1989-06-01 00:00:00
abstract::Structure-guided approaches to HIV-1 vaccine design depend on knowledge of the presentation of neutralizing epitopes on gp120, such as the epitope for the broadly neutralizing mAb b12. Here, we characterized predicted three-dimensional structures of functionally diverse gp120 proteins in their b12-bound conformation, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.06.024
更新日期:2012-10-25 00:00:00
abstract::Determinants of temperature sensitivity and/or attenuation in Sabin type 1 poliovirus reside in the 5' NTR and coding sequences of the capsid proteins and viral RNA polymerase, 3D(pol). Previous studies have implicated at least two mutations in 3D(pol) of Sabin 1 vaccine strain [PV1(S)], including a Y73H change, as co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0354
更新日期:2000-06-20 00:00:00
abstract::Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.031
更新日期:2007-04-10 00:00:00
abstract::In this study, we analyzed the replication and budding sites of severe acute respiratory syndrome coronavirus (SARS-CoV) at early time points of infection. We detected cytoplasmic accumulations containing the viral nucleocapsid protein, viral RNA and the non-structural protein nsp3. Using EM techniques, we found that ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.027
更新日期:2007-05-10 00:00:00
abstract::Expansion of CHIKV outbreaks with appearance of novel mutations are reported from many parts of the world. Two novel mutations viz. E1:K211E and E2:V264A in background of E1:226A are recently identified from Aedes aegypti dominated areas of India. In this study, the role of these mutations in modulation of infectivity...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.025
更新日期:2016-10-01 00:00:00
abstract::Adenovirus vectors are known to induce certain genes and impact innate response networks, but a broad understanding of this process and its mechanisms is currently lacking. For this reason, we chose to investigate and characterize Ad innate immunity using homogeneous, primary MEF cells replete with all the elements of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.041
更新日期:2007-02-20 00:00:00
abstract::Dengue-2 virus strains from different locations were compared by T1-RNAse-resistant oligonucleotide fingerprinting and antigen signature analysis. The latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactiv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90457-5
更新日期:1986-10-30 00:00:00
abstract::A series of nucleotide substitutions (G46----C; C47----A; C315----U; U317----C) were introduced into the virulence modulating region of the intermediate strain of potato spindle tuber viroid (PSTVd) in order to examine their effect upon viroid infectivity and pathogenicity with the presence of all four mutations resul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90468-5
更新日期:1992-04-01 00:00:00
abstract::Efforts to generate a vaccine to prevent infection by human immunodeficiency virus (HIV) have focused on inducing neutralizing antibodies. However, cytotoxic T-lymphocyte (CTL) responses are a major immune defense mechanism required for recovery from many different virus infections. Since CTL epitopes can be defined b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90504-i
更新日期:1992-06-01 00:00:00
abstract::The unordered N-termini of parvovirus capsid proteins (Nt) are translocated through a channel at the icosahedral five-fold axis to serve for virus traffick. Heterologous peptides were genetically inserted at the Nt of MVM to study their functional tolerance to manipulations. Insertion of a 5T4-single-chain antibody at...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.05.025
更新日期:2012-10-10 00:00:00
abstract::We examined the molecular epidemiology and evolution of Ross River (RR) virus in Australia and the Pacific Islands. Nucleotide sequences of the E2 and E3 genes of five RR virus strains revealed remarkable conservation between 1959 and 1989 with a maximum divergence of only 3.3%. Sequence data from a 505-base pair frag...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1449
更新日期:1995-09-10 00:00:00
abstract::We have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to E1 but not to heterologous oncogenes such as c-myc, N-ras, or polyoma middle t (Sircar et al. (1988) Oncogene 3, 725-728). DNA tumor virus oncogenes have been suggested to transform cells via the common mechanis...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90180-w
更新日期:1992-11-01 00:00:00
abstract::Hemorrhagic fever viruses are associated with rapidly progressing severe disease with high case fatality, making them of public health and biothreat importance. Effective antivirals are not available for most of the members of this diverse group of viruses. A broad spectrum strategy for antiviral development would be ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.032
更新日期:2009-09-15 00:00:00
abstract::Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutraliz...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.12.014
更新日期:2013-03-15 00:00:00
abstract::Enteroviruses (EV) frequently infect the central nervous system (CNS) and induce neurological diseases. Although the CNS is composed of many different cell types, the spectrum of tropism for each EV is considerable. These viruses have the ability to completely shut down host translational machinery and are considered ...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2010.12.014
更新日期:2011-03-15 00:00:00
abstract::Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) is expressed on the membrane of B-lymphocytes and blocks B cell receptor (BCR) signaling in EBV-transformed B-lymphocytes in vitro. The LMP2A amino-terminal domain, which is essential for the LMP2A-mediated block of B cell signal transduction, contains eight ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9964
更新日期:1999-10-25 00:00:00
abstract::Information about the basic biological properties of human rhinovirus-C (HRV-C) viruses is lacking due to difficulties with culturing these viruses. Our objective was to develop a cell culture system to grow HRV-C. Epithelial cells from human sinuses (HSEC) were differentiated at air-liquid interface (ALI). Differenti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.002
更新日期:2013-02-05 00:00:00
abstract::We have previously shown that the late promoter of the human papovavirus BK (prototype) is contained within the three 68-bp repeats and a 66-bp region to the late side of the repeats which together constitute the early promoter enhancer. We have now carried out unidirectional deletion and linker scan analyses of these...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90053-6
更新日期:1989-03-01 00:00:00
abstract::Insertion of a functional splicing cassette into a construct containing the HIV-1 poly(A) site followed by the adenovirus L3 poly(A) site results in both specific stimulation of 3' end processing at the HIV-1 site and an increase in the steady-state levels of RNA processed at both sites. To further evaluate this influ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0556
更新日期:1996-10-15 00:00:00
abstract::We have proposed that chronic infection of keratinocytes by HPV modifies the expression of potentially important cytokines by interfering with the NF-kappaB signal pathway. We evaluated the constitutive and IL-1beta-induced expression of GM-CSF and TNF-alpha and the expression/activity of NF-kappaB in HPV+ and HPV- ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1468
更新日期:2002-07-05 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.09.006
更新日期:2021-01-02 00:00:00
abstract::To investigate the role of Tobacco vein banding mosaic virus (TVBMV) 3'-UTR in virus systemic infection, three types of deletions were introduced into TVBMV infectious clone pCaTVBMV-GFP. Mutants with deletions at the nucleotide position 8-42, 43-141, or 163-174 in the 3'-UTR failed to cause systemic infection in N. b...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.11.001
更新日期:2019-01-15 00:00:00
abstract::Barley stripe mosaic virus (BSMV) has a tripartite genome comprising RNAs designated alpha, beta, and gamma, which collectively encode seven polypeptides. We show here that an antiserum raised against an abundant disease-specific protein from BSMV-infected plants reacts specifically with the viral beta b gene product ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90559-a
更新日期:1990-08-01 00:00:00
abstract::It has previously been shown that a far-upstream region of the herpes simplex virus immediate early (IE) gene 3 increases the expression of a linked thymidine kinase (TK) gene and also contains sequences which respond to stimulation of transcription by a virion component. The effects of altering the orientation and di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90238-1
更新日期:1984-09-01 00:00:00
abstract::Multiplication of frog virus 3 (FV 3) occurs in mammalian cells provided they are incubated at temperatures lower than 33 degrees. The expression of the viral genome at supraoptimal temperatures was followed by analyzing the polypeptides produced in CHO-infected cells and comparing with those obtained under restrictiv...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90138-8
更新日期:1986-07-30 00:00:00
abstract::The interaction between the viral envelope protein gp120 and the cellular surface antigen CD4 is a key event in HIV-1 infection. Reciprocal high affinity binding sites have been located in the first domain of CD4 and in the carboxy-terminal region of gp120, respectively. Upon infection, the membranes of the target cel...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90553-n
更新日期:1991-12-01 00:00:00
abstract::LP-BM5, a retroviral isolate, induces a disease featuring an acquired immunodeficiency syndrome termed murine AIDS (MAIDS). Many of the features of the LP-BM5-initiated disease are shared with HIV/AIDS. Our lab has shown that the interaction of B and CD4 T cells that is central to MAIDS pathogenesis requires ligation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.002
更新日期:2007-03-30 00:00:00