Analysis of the virulence modulating region of potato spindle tuber viroid (PSTVd) by site-directed mutagenesis.

abstract：:Primary strains of human immunodeficiency virus type 1 (HIV-1) are known to adapt to replication in cell lines in vitro by becoming sensitive to soluble CD4 (sCD4) and neutralizing antibodies (NAb). T-cell lines favor isolation of variants that use CXCR4 as a co-receptor, while primary isolates predominantly use CCR5....

journal_title：Virology

authors： Pugach P,Kuhmann SE,Taylor J,Marozsan AJ,Snyder A,Ketas T,Wolinsky SM,Korber BT,Moore JP

更新日期：2004-03-30 00:00:00

abstract：:Hemagglutination is an established method but has not been used previously to determine the efficacy of virus binding to a specific cellular receptor. Here we have utilized CD46-expressing erythrocytes from a transgenic mouse to establish whether and to what extent the species B adenoviruses (Ads) as well as Ad37 and ...

journal_title：Virology

authors： Andersson EK,Mei YF,Wadell G

更新日期：2010-06-20 00:00:00

abstract：:We examined the molecular epidemiology and evolution of Ross River (RR) virus in Australia and the Pacific Islands. Nucleotide sequences of the E2 and E3 genes of five RR virus strains revealed remarkable conservation between 1959 and 1989 with a maximum divergence of only 3.3%. Sequence data from a 505-base pair frag...

journal_title：Virology

authors： Sammels LM,Coelen RJ,Lindsay MD,Mackenzie JS

更新日期：1995-09-10 00:00:00

abstract：:The polycation DEAE-dextran increased the binding of VSV to BHK cells about fourfold over a wide range of VSV concentrations. The same proportion of bound virions was internalized by the cell in the presence or absence of DEAE-dextran. Viral primary RNA production was increased to the equivalent of a 4- to 4.5-fold in...

journal_title：Virology

authors： Bailey CA,Miller DK,Lenard J

更新日期：1984-02-01 00:00:00

abstract：:An infectious and full-length molecular clone of genomic human spumaretrovirus (HSRV) DNA was constructed. The infectivity of the pHSRV13 clone was demonstrated after transfection into susceptible cells by passage of HSRV-specific cytopathic effects as a cell-free culture supernatant, by electron microscopy of HSRV pa...

journal_title：Virology

authors： Löchelt M,Zentgraf H,Flügel RM

更新日期：1991-09-01 00:00:00

abstract：:Packaging of lambda DNA is mediated by the phage-encoded enzyme, terminase, which acts at a site termed cos. cos consists of cosB, the site where terminase binds lambda DNA, and cosN, the site where nicks are introduced to generate the cohesive ends of virion DNA. cos contains multiple binding sites for gpNu1, the sma...

journal_title：Virology

authors： Yeo A,Kosturka LD,Feiss M

更新日期：1990-01-01 00:00:00

abstract：:MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...

journal_title：Virology

authors： Yuen PH,Soong MM,Kissil MS,Wong PK

更新日期：1984-01-30 00:00:00

abstract：:Alpha human papillomaviruses (HPVs) are among the most common sexually transmitted agents of which a subset causes cervical neoplasia and cancer in humans. Alpha-PVs have also been identified in non-human primates although few studies have systematically characterized such types. We cloned and characterized 10 distinc...

journal_title：Virology

authors： Chen Z,van Doorslaer K,DeSalle R,Wood CE,Kaplan JR,Wagner JD,Burk RD

更新日期：2009-10-25 00:00:00

abstract：:Alphaherpesviruses comprise closely related viruses of man and animal, including herpes simplex virus, varicella-zoster virus and pseudorabies virus (PRV). Here, using methyl-beta-cyclodextrin and fluorescently tagged PRV, we directly show that depletion of cholesterol from the plasma membrane of host cells significan...

journal_title：Virology

authors： Desplanques AS,Nauwynck HJ,Vercauteren D,Geens T,Favoreel HW

更新日期：2008-07-05 00:00:00

abstract：:Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the trans...

journal_title：Virology

authors： Ruiz A,Lau D,Mitchell RS,Hill MS,Schmitt K,Guatelli JC,Stephens EB

更新日期：2010-10-25 00:00:00

abstract：:Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...

journal_title：Virology

authors： Noble AG,Lee GT,Sprague R,Parish ML,Spear PG

更新日期：1983-08-01 00:00:00

abstract：:The DNAs from the granulosis viruses of Plodia interpunctella and Pieris rapae were clearly distinguishable based on restriction endonuclease fragment patterns. By sizing single- and double-digestion products approximate molecular weights of 72 x 10(2) and 75 x 10(6), respectively, were estimated for the viral genomes...

journal_title：Virology

authors： Tweeten KA,Bulla LA Jr,Consigli RA

更新日期：1980-07-30 00:00:00

abstract：:Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are local...

journal_title：Virology

authors： Cheng L,Huang X,Li X,Xiong W,Sun W,Yang C,Zhang K,Wang Y,Liu H,Huang X,Ji G,Sun F,Zheng C,Zhu P

更新日期：2014-02-01 00:00:00

abstract：:Five monoclonal antibodies (MAbs) to porcine group (gp) C rotaviruses (Cowden and Ah strains) reactive with both gp A and C rotaviruses in cell culture immunofluorescence (CCIF) tests were produced and characterized. These MAbs reacted with three strains of gp A and two strains of gp C rotaviruses in a CCIF test and w...

journal_title：Virology

authors： Tsunemitsu H,Ojeh CK,Jiang B,Simkins RA,Weilnau PA,Saif LJ

更新日期：1992-11-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in cont...

journal_title：Virology

authors： Mahmoud NF,Jasirwan C,Kanemoto S,Wakata A,Wang B,Hata Y,Nagamata S,Kawabata A,Tang H,Mori Y

更新日期：2016-03-01 00:00:00

abstract：:The nucleotide sequence of two variants of coconut tinangaja viroid (CTiV) were obtained. Both sequence variants are 254 nucleotide residues in size but differ in sequence at two positions. In comparisons with other viroids, the sequences and proposed secondary structure of CTiV show most homology with the 246 nucleot...

journal_title：Virology

authors： Keese P,Osorio-Keese ME,Symons RH

更新日期：1988-02-01 00:00:00

abstract：:This study was initiated to evaluate the in vivo infectivity and pathogenicity of a group of recombinant feline leukemia viruses (rFeLVs) previously generated by in vitro forced recombination between a FeLV subgroup A virus (FeLV-A) and an endogenous FeLV (enFeLV) envelope (env) element (Sheets et al., 1992, Virology ...

journal_title：Virology

authors： Pandey R,Bechtel MK,Su Y,Ghosh AK,Hayes KA,Mathes LE,Roy-Burman P

更新日期：1995-12-20 00:00:00

abstract：:Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...

journal_title：Virology

authors： Weldon RA Jr,Sarkar P,Brown SM,Weldon SK

更新日期：2003-09-15 00:00:00

abstract：:Friend murine leukemia virus (Fr-MLV) produces unspliced mRNAs, as well as singly-spliced mRNAs by which the Env protein is generated. We investigated the role of the intron within the Fr-MLV gene in Env expression using vectors with serially truncated introns. The up-regulatory regions, the 361-878 and the 5135-5399 ...

journal_title：Virology

authors： Yamamoto N,Takase-Yoden S

更新日期：2009-03-01 00:00:00

abstract：:L83V-related variants of human papillomavirus (HPV) 16 E6, exemplified by the Asian-American variant Q14H/H78Y/L83V, were shown to be more prevalent than E6 prototype in progressing lesions and cervical cancer. We evaluated functions relevant to carcinogenesis for the E6 variants L83V, R10/L83V and Q14H/H78Y/L83V as w...

journal_title：Virology

authors： Zehbe I,Richard C,DeCarlo CA,Shai A,Lambert PF,Lichtig H,Tommasino M,Sherman L

更新日期：2009-01-05 00:00:00

abstract：:The nucleocapsid (N) protein genes of vesicular stomatitis virus, serotypes New Jersey and Indiana, were expressed in Escherichia coli BL21(DE3) transformed by the recombinant expression vector pET-3a containing N genes under the control of the T7 RNA polymerase promoter. Following induction with isopropylthiogalactop...

journal_title：Virology

authors： Das T,Banerjee AK

更新日期：1993-03-01 00:00:00

abstract：:Glycoprotein B (gB, gpUL55) is a major antigen for the induction of neutralizing antibodies against human cytomegalovirus, making it an attractive antigen for active and passive immunoprophylaxis. The immunodominant region on gB is the antigenic domain 1 (AD-1), a complex structure which requires a minimal linear amin...

journal_title：Virology

authors： Schoppel K,Hassfurther E,Britt W,Ohlin M,Borrebaeck CA,Mach M

更新日期：1996-02-01 00:00:00

abstract：:Murine gammaherpesvirus 68 (MHV68) is used as a model to study gammaherpesvirus pathogenesis both in tissue culture systems and in vivo. We used a gene-trapping approach to get insight into cellular factors involved in MHV68 infection. By generating a library of gene-trapped CHO cells, we were able to isolate several ...

journal_title：Virology

authors： Jarousse N,Coscoy L

更新日期：2008-04-10 00:00:00

abstract：:Rice dwarf virus (RDV) spreads, along actin-based filopodia, as one of the ways of its transport, from infected to non-infected cells of its vector insect via tubules that are composed of the viral nonstructural protein Pns10 and contain viral particles. An inhibitor that perturbs the endomembrane system, namely, bref...

journal_title：Virology

authors： Wei T,Shimizu T,Omura T

更新日期：2008-03-15 00:00:00

abstract：:Two noncomplementing vaccinia virus temperature-sensitive mutants, Cts8 and Cts26, were mapped to the A3L gene, which encodes the major virion structural protein, 4b. The two ts mutants display normal patterns of gene expression, DNA replication, telomere resolution, and protein processing during infection. Morphogene...

journal_title：Virology

authors： Kato SE,Strahl AL,Moussatche N,Condit RC

更新日期：2004-12-05 00:00:00

abstract：:The utilization of transgenic plants expressing recombinant antigens to be used in the formulation of experimental immunogens has been recently communicated. We report here the development of transgenic plants of alfalfa expressing the structural protein VP1 of foot and mouth disease virus (FMDV). The presence of the ...

journal_title：Virology

authors： Wigdorovitz A,Carrillo C,Dus Santos MJ,Trono K,Peralta A,Gómez MC,Ríos RD,Franzone PM,Sadir AM,Escribano JM,Borca MV

更新日期：1999-03-15 00:00:00

abstract：:To better understand the role of specific residues within the duck hepatitis B virus (DHBV) pre-S protein in neutralization and infectivity, we have selected and identified pre-S variants which escape neutralization. A highly neutralizing monoclonal antibody (Mab 900) which recognizes an epitope 83IPQPQWTP90 localized...

journal_title：Virology

authors： Sunyach C,Chassot S,Jamard C,Kay A,Trepo C,Cova L

更新日期：1997-08-04 00:00:00

abstract：:Infectious bronchitis virus (IBV) infection and associated illness may be dramatically modified by passive transfer of immune T lymphocytes. Lymphocytes collected 10 days postinfection were transferred to naive chicks before challenge with virus. As determined by respiratory illness and viral load, transfer of syngene...

journal_title：Virology

authors： Seo SH,Pei J,Briles WE,Dzielawa J,Collisson EW

更新日期：2000-03-30 00:00:00

abstract：:Putative replication complexes of cauliflower mosaic virus (CaMV) which resemble hepatitis B virus replication complexes in several respects have been identified. The CaMV replication complexes contain a heterogeneous population of minus-strand DNA ranging in size from less than 600 nucleotides to full genome length o...

journal_title：Virology

authors： Marsh L,Kuzj A,Guilfoyle T

更新日期：1985-05-01 00:00:00

abstract：:The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this pr...

journal_title：Virology

authors： MacFarlane SA,Shanks M,Davies JW,Zlotnick A,Lomonossoff GP

更新日期：1991-07-01 00:00:00