Analysis of the nucleotide sequence of bean pod mottle virus middle component RNA.

Abstract:

:The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this protein have been identified by comparison with the known three-dimensional structure of the virion and cleavage at these sites would lead to a range of products consistent with those observed during processing of the M RNA-encoded polyproteins in vitro. We have performed computer-aided searches for reiterated sequences within BPMV M RNA which might explain why ordered RNA is visible in the electron density map of BPMV middle component particles (Chen, Z., Stauffacher, C. V., Li, Y., Schmidt, T., Bomu, W., Kamer, G., Shanks, M., Lomonossoff, G., and Johnson, J. E., 1989, Science 245, 154-159). These searches revealed both the presence of overrepresented pentameric sequences and a consensus sequence which was repeated 15 times within the RNA sequence.

journal_name

Virology

journal_title

Virology

authors

MacFarlane SA,Shanks M,Davies JW,Zlotnick A,Lomonossoff GP

doi

10.1016/0042-6822(91)90155-5

subject

Has Abstract

pub_date

1991-07-01 00:00:00

pages

405-9

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

183

pub_type

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