In vitro translation of cucumoviral satellites. II. CARNA 5 from cucumber mosaic virus strain S and SP6 transcripts of cloned (S)CARNA 5 cDNA produce electrophoretically comigrating protein products.

abstract：:We have developed a sensitive assay for DNA replication in mammalian cells that enables us to detect replicated DNA fragments of human cytomegalovirus. A 1/100 portion of DNA extracted from 1000 microinjected cells was subjected to polymerase chain reaction after digestion with appropriate restriction enzymes to diffe...

journal_title：Virology

authors： Watanabe S,Yamaguchi N

更新日期：1993-01-01 00:00:00

abstract：:The single-stranded DNA-binding protein LEF-3 of Autographa californica multinucleocapsid nucleopolyhedrovirus consists of 385 amino acid residues, forms oligomers, and promotes Mg2+-independent unwinding of DNA duplexes and annealing of complementary DNA strands. Partial proteolysis revealed that the DNA-binding doma...

journal_title：Virology

authors： Mikhailov VS,Okano K,Rohrmann GF

更新日期：2006-03-15 00:00:00

abstract：:Coinfection of tobacco plants with potato virus X (PVX) and any of several members of the potyvirus group causes a synergistic disease characterized by a dramatic increase in symptom severity correlated with a 3- to 10-fold increase in the accumulation of PVX in the first systemically infected leaves. We have recently...

journal_title：Virology

authors： Shi XM,Miller H,Verchot J,Carrington JC,Vance VB

更新日期：1997-04-28 00:00:00

abstract：:A new strain of wound tumor virus (WTV) has been isolated from a periwinkle plant (Catharanthus roseus) that was among several used as bait plants in a blueberry field. The 12 segments of double-stranded RNA of the viral genome were isolated directly from infected tissue and found to have mobilities through agarose ge...

journal_title：Virology

authors： Hillman BI,Anzola JV,Halpern BT,Cavileer TD,Nuss DL

更新日期：1991-12-01 00:00:00

abstract：:Based on our previous analysis of HIV-1 isolates from Cameroon, we constructed a full-length infectious molecular clone from a primary isolate belonging to the CRF02.AG group of recombinant viruses which dominate the HIV-epidemic in West and Central Africa. The virus derived by transfection of the proviral clone pBD6-...

journal_title：Virology

authors： Tebit DM,Zekeng L,Kaptué L,Kräusslich HG,Herchenröder O

更新日期：2003-09-01 00:00:00

abstract：:The HIV-1 Nef protein causes a decrease in major histocompatibility complex (MHC) class I and CD4 molecule expression on the cell surface. To determine if Nef can affect components of the innate immune response, we assessed the ability of Nef to alter the cell surface expression of human CD1d. In cells co-expressing C...

journal_title：Virology

authors： Cho S,Knox KS,Kohli LM,He JJ,Exley MA,Wilson SB,Brutkiewicz RR

更新日期：2005-07-05 00:00:00

abstract：:Dipeptidyl peptidase 4 (DPP4) is a receptor for MERS-CoV. The soluble form of DPP4 (sDPP4) circulates systematically and can competitively inhibit MERS-CoV entry into host cells. Here, we measured the concentration of sDPP4 in the plasma and sputa of 14 MERS-CoV-infected patients of various degrees of disease severity...

journal_title：Virology

authors： Inn KS,Kim Y,Aigerim A,Park U,Hwang ES,Choi MS,Kim YS,Cho NH

更新日期：2018-05-01 00:00:00

abstract：:nsP3 is one of four viral nonstructural proteins required for RNA replication of Sindbis virus. In this report, post-translational modifications of nsP3 which occur in both vertebrate and mosquito cell cultures have been examined. In pulse-chase experiments, analyzed by immunoprecipitation and sodium dodecyl sulfate-p...

journal_title：Virology

authors： Li GP,La Starza MW,Hardy WR,Strauss JH,Rice CM

更新日期：1990-11-01 00:00:00

abstract：:Ultraviolet absorption spectra and difference spectra were determined for barley stripe mosaic virus (BSMV) virions and protein and for tobacco mosaic virus (TMV) virions and protein in buffer and in sodium dodecyl sulfate (SDS). The absorbance at 260 nm of BSMV virions disrupted in SDS was 92% of that of intact virio...

journal_title：Virology

authors： Brakke MK

更新日期：1979-10-15 00:00:00

abstract：:The tegument protein pp28 of human cytomegalovirus (HCMV) is essential for the assembly of infectious HCMV virions, but how it functions during the process of HCMV tegumentation and envelopment remains unclear. By using live cell fluorescence resonance energy transfer (FRET) microscopy and yeast two-hybrid assays, we ...

journal_title：Virology

authors： Liu Y,Cui Z,Zhang Z,Wei H,Zhou Y,Wang M,Zhang XE

更新日期：2009-05-25 00:00:00

abstract：:In this study, we show that adenoviral infection induced accumulation of the sphingolipid ceramide in a dose- and time-dependent manner. This accumulation preceded cell lysis, occurred in the absence of biochemical evidence of apoptosis, and was derived from de novo synthesis of ceramide. An adenovirus mutant that lac...

journal_title：Virology

authors： Kanj SS,Dandashi N,El-Hed A,Harik H,Maalouf M,Kozhaya L,Mousallem T,Tollefson AE,Wold WS,Chalfant CE,Dbaibo GS

更新日期：2006-02-05 00:00:00

abstract：:We report here the nonenzymatic self-ligation of transcripts corresponding to the peach latent mosaic viroid (PLMVd). This is the first description of this process with viroid sequences, although it has been reported to occur with human hepatitis delta virus RNA. Self-ligation occurs when the 5'-hydroxyl and the 2',3'...

journal_title：Virology

authors： Lafontaine D,Beaudry D,Marquis P,Perreault JP

更新日期：1995-10-01 00:00:00

abstract：:We have previously demonstrated that the bZIP transcription factor CREB-2, also called ATF-4, trans-activates, in association with the viral protein Tax, the human T-cell leukemia virus type I (HTLV-I) promoter. In this study, we have examined whether CREB-2 acetylation affects transcriptional activation mediated by T...

journal_title：Virology

authors： Gachon F,Devaux C,Mesnard JM

更新日期：2002-08-01 00:00:00

abstract：:The propensity of retroviruses to rapidly establish persistent infections poses a formidable problem in vaccination strategies. In the current study, we use a live attenuated vaccine to study protection against acute and persistent Friend virus infections in mice. Adoptive transfers of immune CD8(+) T cells combined w...

journal_title：Virology

authors： Dittmer U,Hasenkrug KJ

更新日期：2000-06-20 00:00:00

abstract：:The longest hepatitis B virus transcript is a 3.9-kb mRNA whose function remained unclear. In this study, we wished to identify the translation products and physiological role of this viral transcript. This transcript initiates from the X promoter region ignoring the inefficient and noncanonical viral polyadenylation ...

journal_title：Virology

authors： Doitsh G,Shaul Y

更新日期：2003-05-10 00:00:00

abstract：:Dengue 2 (DEN 2) virus strains collected from dengue hemorrhagic fever (DHF) patients and Aedes aegypti mosquitoes in Thailand, Burma, and Vietnam over a 25-year period have been analyzed by computer assisted T1-RNase-resistant oligonucleotide fingerprinting. Fifty-seven DEN 2 virus strains of the Thailand topotype we...

journal_title：Virology

authors： Trent DW,Grant JA,Monath TP,Manske CL,Corina M,Fox GE

更新日期：1989-10-01 00:00:00

abstract：:While HPV 16 variant lineages have been well characterized, the knowledge about HPV 18 variants is limited. In this study, HPV 18 nucleotide variations in the E2 hinge region were characterized by sequence analysis in 47 control and 51 tumor specimens. Fifty of these specimens were randomly selected for sequencing of ...

journal_title：Virology

authors： Arias-Pulido H,Peyton CL,Torrez-Martínez N,Anderson DN,Wheeler CM

更新日期：2005-07-20 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) is an economically important cause of post-weaning multisystemic wasting syndrome (PMWS) in weanling piglets. Current commercial vaccines against PCV2 are highly effective. Yet, a recurring emergence of new genotypes in vaccinated herds necessitates a better understanding of protective...

journal_title：Virology

authors： Ilha M,Nara P,Ramamoorthy S

更新日期：2020-01-15 00:00:00

abstract：:IL-17-producing (Th17) and regulatory T (Treg) cells have been well established in the pathogenesis of many inflammatory diseases. To assess whether Th17 and Treg were altered in acute virus-induced myocarditis (AVMC) mice, we assessed Th17/Treg functions on different levels in AVMC. It was shown that the expression o...

journal_title：Virology

authors： Xie Y,Chen R,Zhang X,Chen P,Liu X,Xie Y,Yu Y,Yang Y,Zou Y,Ge J,Chen H

更新日期：2011-12-05 00:00:00

abstract：:The envelope (E) protein of dengue virus (DENV) is composed of three domains (EDI, EDII, EDIII) and is the main target of neutralizing antibodies. Many monoclonal antibodies that bind EDIII strongly neutralize DENV. However in vitro studies indicate that anti-EDIII antibodies contribute little to the neutralizing pote...

journal_title：Virology

authors： Williams KL,Wahala WM,Orozco S,de Silva AM,Harris E

更新日期：2012-07-20 00:00:00

abstract：:An essentially complete DNA copy of the ambisense S RNA species of Punta Toro (PT) phlebovirus (T. Ihara, H. Akashi, and D.H.L. Bishop, 1984, Virology 136, 293-306) has been inserted in either orientation into Autographa californica nuclear polyhedrosis baculovirus (AcNPV) in lieu of the 5' coding region of the AcNPV ...

journal_title：Virology

authors： Overton HA,Ihara T,Bishop DH

更新日期：1987-04-01 00:00:00

abstract：:A cDNA was constructed to encode an internally-truncated version of negative-sense genomic RNA (vRNA) of respiratory syncytial virus (RSV) containing (in 3' to 5' order) the 3' vRNA leader region, the complement of the open reading frame for bacterial chloramphenicol acetyl transferase (CAT) under the control of RSV g...

journal_title：Virology

authors： Collins PL,Mink MA,Hill MG 3rd,Camargo E,Grosfeld H,Stec DS

更新日期：1993-07-01 00:00:00

abstract：:Primary varicella-zoster virus (VZV) infection of humans may result in latent infection of sensory neurons in the peripheral nervous system. To examine the interaction of VZV with the sensory neuron we infected immunochemically defined human neurons with cell-associated VZV. Utilizing double-label immunofluorescence t...

journal_title：Virology

authors： Wigdahl B,Rong BL,Kinney-Thomas E

更新日期：1986-07-30 00:00:00

abstract：:The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding ...

journal_title：Virology

authors： Putkonen P,Quesada-Rolander M,Leandersson AC,Schwartz S,Thorstensson R,Okuda K,Wahren B,Hinkula J

更新日期：1998-10-25 00:00:00

abstract：:3H-labelled complementary DNA (cDNA) was reverse transcribed from the RNA of three biologically distinct isolates of bean yellow mosaic virus (BYMV-G, Q, and S) by the random primer method of Taylor et al. [Biochim. Biophys. Acta 442, 324-330 (1976)]. Excess virus RNA was hybridized with each of the cDNAs and percenta...

journal_title：Virology

authors： Abu-Samah N,Randles JW

更新日期：1981-04-30 00:00:00

abstract：:Revertants were isolated from the protease activation mutant of Sendai virus, F1-R, which causes a systemic infection in mice. The fusion (F) glycoprotein of F1-R is susceptible to activation cleavage by ubiquitous cellular proteases and is thus responsible for pantropism in mice (Tashiro et al., 1988. Virology 165, 5...

journal_title：Virology

authors： Tashiro M,James I,Karri S,Wahn K,Tobita K,Klenk HD,Rott R,Seto JT

更新日期：1991-09-01 00:00:00

abstract：:Expression of Kaposi's sarcoma herpesvirus vFLIP, a potent activator of NFkB signaling, promotes latency. Inhibition of NFkB signaling promotes lytic reactivation. We previously reported that lytic inducer, RTA, inhibits vFLIP induced NFkB signaling by inducing the degradation of vFLIP via the proteasome. Here we repo...

journal_title：Virology

authors： Chmura JC,Herold K,Ruffin A,Atuobi T,Fabiyi Y,Mitchell AE,Choi YB,Ehrlich ES

更新日期：2017-01-15 00:00:00

abstract：:Measles virus transcription generates multiple P/C gene-specific mRNAs by a process which has been termed editing. In one of these mRNAs, the cotranscriptional addition of a single nontemplated G residue allows translational access to the V protein reading frame. The protein translated from this mRNA has been called V...

journal_title：Virology

authors： Liston P,Briedis DJ

更新日期：1994-01-01 00:00:00

abstract：:Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...

journal_title：Virology

authors： Noble AG,Lee GT,Sprague R,Parish ML,Spear PG

更新日期：1983-08-01 00:00:00

abstract：:An effective type I interferon (IFN-alpha/beta) response is critical for the control of many viral infections. Using an oncolytic strain of vesicular stomatitis virus, we have examined the cross-talk between virus-induced apoptosis and initiation of innate immune response. The intrinsic apoptotic cascade, specifically...

journal_title：Virology

authors： Sharif-Askari E,Nakhaei P,Oliere S,Tumilasci V,Hernandez E,Wilkinson P,Lin R,Bell J,Hiscott J

更新日期：2007-08-15 00:00:00