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Ceramide regulates SR protein phosphorylation during adenoviral infection.

Abstract：

:In this study, we show that adenoviral infection induced accumulation of the sphingolipid ceramide in a dose- and time-dependent manner. This accumulation preceded cell lysis, occurred in the absence of biochemical evidence of apoptosis, and was derived from de novo synthesis of ceramide. An adenovirus mutant that lacks the adenovirus death protein (ADP) produced ceramide accumulation in the absence of cell lysis. This suggested that ceramide accumulation was either driven by adenovirus or was a cellular stress response but was unlikely a result of cell death. The use of inhibitors of ceramide synthesis resulted in a significant delay in cell lysis, suggesting that ceramide was necessary for the lytic phase of the infection. Serine/arginine-rich (SR) proteins were dephosphorylated during the late phase of the viral cycle, and inhibitors of ceramide synthesis reversed this. These findings suggest that adenovirus utilizes the ceramide pathway to regulate SR proteins during infection.

journal_name

Virology

journal_title

Virology

authors

Kanj SS,Dandashi N,El-Hed A,Harik H,Maalouf M,Kozhaya L,Mousallem T,Tollefson AE,Wold WS,Chalfant CE,Dbaibo GS

doi

10.1016/j.virol.2005.09.060

subject

Has Abstract

pub_date

2006-02-05 00:00:00

pages

280-9

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(05)00640-9

journal_volume

345

pub_type

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