Abstract:
:The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfection. Selection of cells in G418 led to the isolation of resistant colonies which were propagated in culture. Four cell lines termed FE-A, FEH 18L, FEP18-5, and FEP18-11 have been maintained in culture for 1 1/2-2 years and were selected for further analysis. In all cases the viral DNA was integrated into the cellular genome and the early genes were transcribed, including RNA complementary to the E2, E6, and E7 open reading frames. Radioimmunoprecipitation showed that all cell lines synthesized the E6 and E7 proteins. However, none of the cell lines tested were tumorigenic. The differentiation capacity of these cells was analyzed by assessing their ability to proliferate clonally after exposure to 1.2 mM calcium chloride. All four cell lines were resistant to this stimulus and formed colonies upon return to regular growth medium whereas normal cells differentiated terminally. K6a and K14 keratin RNA expression was down-regulated in the HPV immortalized cell lines compared to primary human epithelial cells.
journal_name
Virologyjournal_title
Virologyauthors
Kaur P,McDougall JKdoi
10.1016/0042-6822(89)90247-xsubject
Has Abstractpub_date
1989-11-01 00:00:00pages
302-10issue
1eissn
0042-6822issn
1096-0341journal_volume
173pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::V-CATH, a cathepsin L-like cysteine protease encoded by the baculovirus Autographa californica M nucleopolyhedrovirus, has been shown to play an essential role in host liquefaction. Similar to cellular cathepsin L, V-CATH is synthesized as an inactive proenzyme and is activated by cleavage of the propeptide. Previous ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1378
更新日期:2002-05-10 00:00:00
abstract::Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.10.031
更新日期:2007-04-10 00:00:00
abstract::We present a case of sexual transmission of HIV-1 predicted to have CXCR4-tropism during male-to-male sexual exposure. Phylogenetic analyses exclude cell-free virus in the seminal plasma of the source partner and possibly point to the seminal cells as the origin of the transmission event. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.010
更新日期:2012-12-05 00:00:00
abstract::A new type of retrovirus (Sm-MTV) released by cultured cells of a spontaneous mammary tumor from a house musk shrew, Suncus murinus, is described. The Sm-MTV is distinct morphologically from type C particles. In spite of certain morphological similarities to type B and type D retroviruses, the Sm-MTV is readily distin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90324-1
更新日期:1985-07-15 00:00:00
abstract::Replication of Tomato bushy stunt virus (TBSV) RNA takes place on the cytosolic membrane surface of peroxisomes in plants and in yeast, a model host. To identify the host proteins involved in assisting the peroxisomal localization of the tombusvirus p33 replication protein, we tested if p33 could bind directly to yeas...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.044
更新日期:2008-09-30 00:00:00
abstract::The Epstein-Barr virus nuclear antigen 2A (EBNA-2A) has been strongly implicated in the EBV-mediated B-cell transformation process. Since EBNA-2A might exert this function through interaction with proteins of the infected cell, we studied the association of EBNA-2A with cellular proteins. Immunoprecipitation of EBNA-2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1406
更新日期:1993-08-01 00:00:00
abstract::Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.02.016
更新日期:2008-05-10 00:00:00
abstract::Monoclonal antibody 1B4, previously shown to be protective in vivo and to cross-react with both virally encoded and normal host cell proteins, was used to screen a lambda gt11 cDNA derived from mRNA harvested from mouse embryo fibroblasts 24 hr after infection with murine cytomegalovirus (MCMV). A 700-bp cDNA was iden...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1227
更新日期:1994-05-01 00:00:00
abstract::Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0638
更新日期:2000-12-05 00:00:00
abstract::Equine infectious anemia virus (EIAV) is a lentivirus that causes persistent infections in horses. We hypothesized that high-avidity CTL specific for nonvariable epitopes might be associated with low viral load and minimal disease in EIAV-infected horses. To test this hypothesis, memory CTL (CTLm) responses were analy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00344-1
更新日期:2003-09-01 00:00:00
abstract::Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90386-3
更新日期:1989-05-01 00:00:00
abstract::Phage PR4 contains a lipid bilayer within the phage protein capsid. The phospholipids of the bilayer are derived from those of the host. We report that phage morphogenesis selects against the unusually bulky phospholipids synthesized by Escherichia coli grown in the presence of various sugar alcohols. These data indic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1004
更新日期:1994-01-01 00:00:00
abstract::The sequences of the 3'-noncoding regions (NCR) of 12 strains of tick-borne encephalitis (TBE) virus were analyzed and found to vary in length from 350 to approximately 750 nucleotides. The size heterogeneity is restricted to a variable region following the stop codon, whereas the most 3'-terminal 350 nucleotides form...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1557
更新日期:1995-10-20 00:00:00
abstract::EBNA-1 is the sole virally specified protein required for replication of the Epstein-Barr virus latency origin and also modulates its own expression. Both of these functions are mediated through specific DNA binding. We created a series of amino acid substitutions across the previously identified DNA-binding domain of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1669
更新日期:1994-12-01 00:00:00
abstract::The P1a protein of the ipomovirus Cucumber vein yellowing virus is one of the self-cleavage serine proteases present in Potyviridae family members. P1a is located at the N-terminal end of the viral polyprotein, and is closely related to potyviral P1 protease. For its proteolytic activity, P1a requires a still unknown ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.12.013
更新日期:2015-02-01 00:00:00
abstract::Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a292l. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recom...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9958
更新日期:1999-10-25 00:00:00
abstract::Multiple RNA species (ASV(1-5)) ranging in size from about 61,000 to 185,000 daltons have been detected in association with sunblotch disease of avocado. The predominant species, ASV(5), with a molecular weight of 61,000 accumulates to concentration approaching 5 S RNA. All RNA species are detected in extracts from sy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90370-0
更新日期:1980-07-15 00:00:00
abstract::Segment reassortment of negative strand viruses is an important mechanism for the development of new virus strains with altered pathogenicity. This study reports on in vitro generation of reassortants between Andes (ANDV) and Sin Nombre (SNV) viruses. Although they both cause hantavirus pulmonary syndrome (HPS), ANDV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.012
更新日期:2004-10-01 00:00:00
abstract::The nucleotide sequence was determined of a 1.6-kb restriction fragment containing the polyhedrin gene of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV). In addition, both the 5' and 3' termini of the polyhedrin mRNA were located in the flanking sequences using S1 mapping procedures. 5' Le...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90031-0
更新日期:1986-09-01 00:00:00
abstract::J-chain-deficient (Jch(-/-)) mice were used to study the role of polymeric IgA (pIgA) in primary disease and protective immunity following genital herpes simplex type 2 (HSV-2) infection. Vaginal IgA in the Jch(-/-) mice was composed primarily of monomeric IgA and was not associated with secretory component (SC). In c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0303
更新日期:2000-05-25 00:00:00
abstract::In situ hybridization of viral DNA fragments to metaphase chromosomes of two chicken T-lymphoblastoid cell lines (MDCC) demonstrated that Marek's disease virus DNA was located in the short arm of chromosome 2 of MDCC-MSB1 and MDCC-RP1 cells and in the short arm of chromosome 4 of MDCC-MSB1 cells. The MDV DNA sequences...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90390-9
更新日期:1986-07-15 00:00:00
abstract::Infectious bronchitis virus (IBV) produces six subgenomic (sg) mRNAs, each containing a 64 nucleotide (nt) leader sequence, derived from the 5' end of the genome by a discontinuous process. Several putative functional domains such as a papain-like proteinase (PL(pro)), main protease (M(pro)), RNA-dependent RNA polymer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.03.032
更新日期:2004-06-20 00:00:00
abstract::A dodecapeptide containing the sequence of the C terminus of the nonstructural polyprotein of Sindbis virus has been synthesized and used to immunize rabbits. The antisera obtained precipitated polypeptides from cells infected with the HR strains of Sindbis or with temperature-sensitive mutants ts11 or ts18. Four diff...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90254-5
更新日期:1985-03-01 00:00:00
abstract::Treatment of HeLa cells with human lymphoblastoid interferon (IFN-alpha) does not inhibit reovirus type 3 protein synthesis during virus infection. In contrast, reovirus translation is blocked by treatment of L cells with mouse IFN-alpha. The (2'-5')A synthetase activity is induced in HeLa cells by IFN-alpha treatment...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90555-7
更新日期:1988-06-01 00:00:00
abstract::West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characteriz...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.006
更新日期:2016-09-01 00:00:00
abstract::We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90491-6
更新日期:1988-02-01 00:00:00
abstract::Three prophage sequences were identified in the Lactobacillus johnsoni strain NCC533. Prophage Lj965 predicted a gene map very similar to those of pac-site Streptococcus thermophilus phages over its DNA packaging and head and tail morphogenesis modules. Sequence similarity linked the putative DNA packaging and head mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0498
更新日期:2000-09-30 00:00:00
abstract::Respiratory syncytial virus (RSV) is a major cause of bronchiolitis and pneumonia in infants and pneumonia virus of mice (PVM) causes similar disease. BALB/c mice are highly susceptible, while C57BL/6 mice are more resistant to PVM. IL-12 was significantly more up-regulated in response to PVM infection in BALB/c than ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.06.020
更新日期:2016-10-01 00:00:00
abstract::In this study, we confirmed the autophagy induced by porcine reproductive and respiratory syndrome virus (PRRSV) in permissive cells and investigated the role of autophagy in the replication of PRRSV. We first demonstrated that PRRSV infection significantly results in the increased double-membrane vesicles, the accumu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.03.022
更新日期:2012-08-01 00:00:00
abstract::The ultrastructure of the mature and immature poxvirus Molluscum contagiosum has been examined by electron microscopy. With freeze-fracturing one fracture plane was observed in the viral envelope. During the conversion of immature into mature virus, particles inside the envelope become partly rearranged into parallel ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90215-x
更新日期:1977-11-01 00:00:00