Molecular characterization of Drosophila cells persistently infected with Flock House virus.

abstract：:Tick-borne flaviviruses are maintained in nature in an enzootic cycle involving a tick vector and a vertebrate host. Thus, the virus replicates in two disparate hosts, each providing selective pressures that can influence virus replication and pathogenicity. To identify viral determinants associated with replication i...

journal_title：Virology

authors： Mitzel DN,Best SM,Masnick MF,Porcella SF,Wolfinbarger JB,Bloom ME

更新日期：2008-11-25 00:00:00

abstract：:Neutralizing sites on bluetongue virus serotype 10 (BTV-10) were investigated with a panel of seven neutralizing monoclonal antibodies (MAbs). Each MAb was coupled to agarose beads and tested against the other MAbs and a nonneutralizing control MAb in a competitive immueprecipitation assay. In addition, neutralization...

journal_title：Virology

authors： Heidner HW,Rossitto PV,MacLachlan NJ

更新日期：1990-06-01 00:00:00

abstract：:Analogous to transmission of human T-cell leukemia virus type 1 (HTLV-1) in vivo, an in vitro cell-to-cell infection model was established by coculturing MT-2 cells as virus donors and HUT78 cells as recipients. At a donor:recipient ratio of 1:2, cell fusion occurred and a new round of HTLV-1 genome replication was in...

journal_title：Virology

authors： Benovic S,Kok T,Stephenson A,McInnes J,Burrell C,Li P

更新日期：1998-05-10 00:00:00

abstract：:In order to study the effect of nef gene expression on viral replication in monocytic cells, we established monocytic (U937 and THP-1) cell transfectants constitutively expressing the human immunodeficiency virus type 1 nef gene. We constructed a plasmid expressing the nef gene derived from an infectious clone, NL432,...

journal_title：Virology

authors： Tsunetsugu-Yokota Y,Matsuda S,Maekawa M,Saito T,Takemori T,Takebe Y

更新日期：1992-12-01 00:00:00

abstract：:The 682-nt satellite DNA (sat-DNA) of Tomato leaf curl virus (TLCV) depends on the helper virus for its replication. In contrast to the strict specificity that exists in each geminivirus for its cognate replication associated protein (Rep), TLCV sat-DNA can utilize Rep encoded by distinct geminiviruses. We have used a...

journal_title：Virology

authors： Lin B,Akbar Behjatnia SA,Dry IB,Randles JW,Rezaian MA

更新日期：2003-01-20 00:00:00

abstract：:In order to investigate the global and dynamic host microRNAs (miRNAs)/messenger RNAs (mRNAs) expression alteration during in vitro acute HCV infection, a comprehensive microarray analysis was performed using human hepatoma cells. Totally, 108 human miRNAs and 1247 mRNAs were identified whose expression levels changed...

journal_title：Virology

authors： Liu X,Wang T,Wakita T,Yang W

更新日期：2010-03-01 00:00:00

abstract：:Ts1, a temperature-sensitive mutant of Moloney murine leukemia virus-TB (MoMuLV-TB), causes a progressive hindlimb paralytic disease in susceptible strains of mice. Previously, it has been shown that a single amino acid substitution, Val-25----Ile in gPr80env, is responsible for the temperature sensitivity, inefficien...

journal_title：Virology

authors： Kamps CA,Lin YC,Wong PK

更新日期：1991-10-01 00:00:00

abstract：:We report the complete nucleotide sequence of the bovine herpesvirus 1 (BHV-1) immediate-early gene encoding BICP4, the homolog of the ICP4 protein of herpes simplex virus. Combined with previous mapping studies, the sequence analysis revealed that the transcript for BICP4 consisted of a noncoding leader RNA (exon 1; ...

journal_title：Virology

authors： Schwyzer M,Vlcek C,Menekse O,Fraefel C,Paces V

更新日期：1993-11-01 00:00:00

abstract：:The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of int...

journal_title：Virology

authors： Simon KO,Cardamone JJ Jr,Whitaker-Dowling PA,Youngner JS,Widnell CC

更新日期：1990-07-01 00:00:00

abstract：:Bluetongue virus (BTV) VP1 protein, a component of the viral RNA-directed RNA polymerase, but not the VP4 or VP6 proteins, was specifically incorporated into baculovirus expressed BTV core-like particles (composed of VP3 and VP7) and BTV virus-like particles (composed of VP2, VP3, VP5, and VP7). The VP1 protein has be...

journal_title：Virology

authors： Loudon PT,Roy P

更新日期：1991-02-01 00:00:00

abstract：:The phospholipid (PL) molecular species compositions of bacteriophages PRD1 and Bam35 as well as their respective hosts were determined quantitatively using liquid chromatography/electrospray ionization mass-spectrometry (LC-ESI-MS) and backed up by gas-chromatographic/mass-spectrometric (GC-MS) analysis of the total ...

journal_title：Virology

authors： Laurinavicius S,Käkelä R,Somerharju P,Bamford DH

更新日期：2004-05-01 00:00:00

abstract：:The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...

journal_title：Virology

authors： Grieder FB,Davis NL,Aronson JF,Charles PC,Sellon DC,Suzuki K,Johnston RE

更新日期：1995-02-01 00:00:00

abstract：:Varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein transactivates expression of VZV promoters. VZV ORF61 is functionally homologous to herpes simplex virus type 1 (HSV-1)-infected cell protein 0 (ICP0); however, amino acid sequence homology of these two proteins is limited to the RING finger domains, a...

journal_title：Virology

authors： Moriuchi H,Moriuchi M,Cohen JI

更新日期：1994-11-15 00:00:00

abstract：:The major DNA-binding protein or infected cell protein 8 (ICP8) encoded by herpes simplex virus exhibits multiple interactions with the cell nucleus in that it interacts with the host cell nuclear matrix and viral DNA molecules as sequential stages in its maturational process (M. P. Quinlan, L. B. Chen, and D. M. Knip...

journal_title：Virology

authors： Gao M,Bouchey J,Curtin K,Knipe DM

更新日期：1988-04-01 00:00:00

abstract：:The ability of some Sendai virus stocks to strongly activate IFNbeta has long been known to be associated with defective-interfering (DI) genomes. We have compared SeV stocks containing various copyback and internal deletion DI genomes (and those containing only nondefective (ND) genomes) for their ability to activate...

journal_title：Virology

authors： Strahle L,Garcin D,Kolakofsky D

更新日期：2006-07-20 00:00:00

abstract：:Avian infectious laryngotracheitis virus (ILTV), a herpesvirus, is a highly contagious pathogen that causes an upper respiratory tract infection in chickens. It is one of the major problems in the poultry industry worldwide. Current vaccines are not satisfactory due to the induction of latent infection. Here we descri...

journal_title：Virology

authors： Guo P,Scholz E,Maloney B,Welniak E

更新日期：1994-08-01 00:00:00

abstract：:Hemorrhagic fever viruses are associated with rapidly progressing severe disease with high case fatality, making them of public health and biothreat importance. Effective antivirals are not available for most of the members of this diverse group of viruses. A broad spectrum strategy for antiviral development would be ...

journal_title：Virology

authors： Spiropoulou CF,Ranjan P,Pearce MB,Sealy TK,Albariño CG,Gangappa S,Fujita T,Rollin PE,Nichol ST,Ksiazek TG,Sambhara S

更新日期：2009-09-15 00:00:00

abstract：:Within the family of Poxviridae, the entomopoxviruses are the most distant relatives of the more well-known and intensively studied orthopoxviruses (vaccinia and variola). The growth of the entomopoxvirus from Amsacta moorei (AmEPV) has been characterized in cell culture and compared to that of vaccinia virus (VV), th...

journal_title：Virology

authors： Winter J,Hall RL,Moyer RW

更新日期：1995-08-20 00:00:00

abstract：:A chloroplast-localized papaya methionine sulfoxide reductase B1 (PaMsrB1) interacting with Papaya ringspot virus (PRSV) NIa-Pro was identified using a Sos recruitment two-hybrid system (SRS). SRS analysis of several deletion mutants of PRSV NIa-Pro and PaMsrB1 demonstrated that the C-terminal (residues 133-239) fragm...

journal_title：Virology

authors： Gao L,Shen W,Yan P,Tuo D,Li X,Zhou P

更新日期：2012-12-05 00:00:00

abstract：:Human Cytomegalovirus (HCMV) infection is compromised in cells lacking p53, a transcription factor that mediates cellular stress responses. In this study we have investigated compromised functional virion production in cells with p53 knocked out (p53KOs). Infectious center assays found most p53KOs released functional ...

journal_title：Virology

authors： Kuan MI,O'Dowd JM,Chughtai K,Hayman I,Brown CJ,Fortunato EA

更新日期：2016-10-01 00:00:00

abstract：:Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cell...

journal_title：Virology

authors： Desport M,Tenaya IW,McLachlan A,McNab TJ,Rachmat J,Hartaningsih N,Wilcox GE

更新日期：2009-10-25 00:00:00

abstract：:Phylogenetic analysis of the N8 neuraminidase (NA) genes from 18 influenza A viruses, representing equine and avian hosts in different geographic locations, revealed three major lineages: (i) currently circulating equine 2 viruses; (ii) avian viruses isolated in the Eurasian region, including A/Equine/Jilin/1/89, a re...

journal_title：Virology

authors： Saito T,Kawaoka Y,Webster RG

更新日期：1993-04-01 00:00:00

abstract：:The tobamoviruses, tobacco mosaic virus (TMV) and Odontoglossum ringspot virus (ORSV), differ in the range of plant species that each can systemically infect. Both viruses systemically infect Nicotiana benthamiana similarly, but differ in the ability to systemically infect tobacco (N. tabacum). ORSV is confined to the...

journal_title：Virology

authors： Hilf ME,Dawson WO

更新日期：1993-03-01 00:00:00

abstract：:The influenza virus mRNAs are structurally similar to cellular mRNAs nevertheless; the virus promotes selective translation of viral mRNAs despite the inhibition of host cell protein synthesis. The infection proceeds normally upon functional impairment of eIF4E cap-binding protein, but requires functional eIF4A helica...

journal_title：Virology

authors： Yángüez E,Rodriguez P,Goodfellow I,Nieto A

更新日期：2012-01-20 00:00:00

abstract：:Clones have been constructed containing full-length cDNA copies of PEBV RNA1 and RNA2, flanked by the CaMV 35 S RNA promoter and the nopaline synthase terminator. The clones are infectious when inoculated onto Nicotiana benthamiana plants. Both the viral RNAs and the virus particles were identified in infected plants....

journal_title：Virology

authors： MacFarlane SA,Gilmer D,Davies JW

更新日期：1992-04-01 00:00:00

abstract：:The presence of anti-Tax antibody responses in human T cell leukemia virus type I (HTLV-I)-infected individuals has been correlated with increased proviral load, increased risk of transmitting infection, and increased risk of developing tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM). In this study...

journal_title：Virology

authors： Lydy SL,Conner ME,Marriott SJ

更新日期：1998-10-10 00:00:00

abstract：:Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which repres...

journal_title：Virology

authors： Godeny EK,Speicher DW,Brinton MA

更新日期：1990-08-01 00:00:00

abstract：:Two minor proteins with molecular masses of 8.5 and 15.5 kDa were identified in feline calicivirus (FCV) virions. Direct sequence analysis showed that the N-terminal sequence of the 8.5-kDa protein was identical to that of the predicted protein encoded by open reading frame 3 (ORF3) of the FCV genome. The N-terminal s...

journal_title：Virology

authors： Sosnovtsev SV,Green KY

更新日期：2000-11-10 00:00:00

abstract：:The complete nucleotide sequence of the RSV cpts-248/404 live attenuated vaccine candidate was determined from cloned cDNA and was compared to that of the RSV A2/HEK7 wild-type, cold-passaged cp-RSV, and cpts-248 virus, which constitute the series of progenitor viruses. RSV cpts-248/404 is more attenuated and more tem...

journal_title：Virology

authors： Firestone CY,Whitehead SS,Collins PL,Murphy BR,Crowe JE Jr

更新日期：1996-11-15 00:00:00

abstract：:Old world hantaviruses, causing hemorrhagic fever with renal syndrome (HFRS), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. T...

journal_title：Virology

authors： Koch J,Liang M,Queitsch I,Kraus AA,Bautz EK

更新日期：2003-03-30 00:00:00