Nucleotide sequence analysis of the respiratory syncytial virus subgroup A cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated virus vaccine candidate.

abstract：:A recombination event at the 3' end of RNA 3 in a pseudorecombinant virus (C1C2T3) having RNAs 1 and 2 from TrK7-cucumber mosaic virus (CMV) and RNA 3 from P-tomato aspermy virus (TAV) was detected by ribonuclease protection assay (RPA). Sequence analysis of the 3' end of RNA 3 of C1C2T3 and of its parental CMV and TA...

journal_title：Virology

authors： Fernández-Cuartero B,Burgyán J,Aranda MA,Salánki K,Moriones E,García-Arenal F

更新日期：1994-09-01 00:00:00

abstract：:Alpacas develop diminished disease following bovine viral diarrhea virus (BVDV) infection compared to cattle. We hypothesized that alpaca and bovine cells have differential permissiveness and responses to BVDV infection. To characterize alpaca testicular (AT) and bovine turbinate (BT) cells BVDV infection permissivene...

journal_title：Virology

authors： Samson HC,Topliff CL,Donis RO,Kelling CL

更新日期：2011-04-25 00:00:00

abstract：:The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant wer...

journal_title：Virology

authors： Murata T,Isomura H,Yamashita Y,Toyama S,Sato Y,Nakayama S,Kudoh A,Iwahori S,Kanda T,Tsurumi T

更新日期：2009-06-20 00:00:00

abstract：:Rotaviruses are the leading cause of severe diarrhea in infants and young children worldwide. Thus, the development of an effective rotavirus vaccine is a major public health goal. This study was performed to identify the gene or genes responsible for rotavirus virulence or host range restriction and attenuation in a ...

journal_title：Virology

authors： Hoshino Y,Saif LJ,Kang SY,Sereno MM,Chen WK,Kapikian AZ

更新日期：1995-05-10 00:00:00

abstract：:To begin to map functional domains of the Sendai P-L RNA polymerase complex we wanted to characterize the P binding site on the Sendai L protein. Analysis of in vitro and in vivo P-L polymerase complex formation with carboxyl-truncations of the L protein showed that the N-terminal half of the protein was required. Sit...

journal_title：Virology

authors： Chandrika R,Horikami SM,Smallwood S,Moyer SA

更新日期：1995-11-10 00:00:00

abstract：:The longest hepatitis B virus transcript is a 3.9-kb mRNA whose function remained unclear. In this study, we wished to identify the translation products and physiological role of this viral transcript. This transcript initiates from the X promoter region ignoring the inefficient and noncanonical viral polyadenylation ...

journal_title：Virology

authors： Doitsh G,Shaul Y

更新日期：2003-05-10 00:00:00

abstract：:Enterovirus A71 (EV-A71) is a neurotropic enterovirus that uses heparan sulfate as an attachment receptor. The molecular determinants of EV-A71-heparan sulfate interaction are unknown. With In silico heparin docking and mutagenesis of all possible lysine residues in VP1, we identified that K162, K242 and K244 are resp...

journal_title：Virology

authors： Tan CW,Sam IC,Lee VS,Wong HV,Chan YF

更新日期：2017-01-15 00:00:00

abstract：:The complete nucleotide sequence of the nucleocapsid (N) gene of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus of plant rhabdoviruses, has been determined from cDNA clones of both the viral genomic and messenger RNAs. The previously identified N gene was adjacent to the 3' leader ...

journal_title：Virology

authors： Wetzel T,Dietzgen RG,Geering AD,Dale JL

更新日期：1994-08-01 00:00:00

abstract：:Infection with many viruses results in the selective shutoff of host protein synthesis. A common target for virus interference with host protein synthesis is the cap-binding protein complex, eIF4F. The large subunit of the complex, eIF4G, is cleaved upon picornavirus (except cardiovirus) infection. Infection with aden...

journal_title：Virology

authors： Gingras AC,Sonenberg N

更新日期：1997-10-13 00:00:00

abstract：:Among 489 bats of 11 species in China, three novel paramyxoviruses [Tuhokovirus 1, 2 and 3 (ThkPV-1, ThkPV-2 and ThkPV-3)] were discovered in 15 Leschenault's rousettes. Phylogenetically, the three viruses are most closely related to Menangle and Tioman virus. Genome analysis showed that their 3'-leader sequences are ...

journal_title：Virology

authors： Lau SK,Woo PC,Wong BH,Wong AY,Tsoi HW,Wang M,Lee P,Xu H,Poon RW,Guo R,Li KS,Chan KH,Zheng BJ,Yuen KY

更新日期：2010-08-15 00:00:00

abstract：:To elucidate the virulence factors of equine herpesvirus-1 (EHV-1), we compared the genomic architecture of the HH1 strain, a Japanese isolate of EHV-1, with that of its four descendant strains obtained by serial passage in bovine kidney (BK) cells, i.e. BK77, BK161, BK271, and BK343. In preliminary studies high-passa...

journal_title：Virology

authors： Kirisawa R,Ui S,Takahashi A,Kawakami Y,Iwai H

更新日期：1994-05-01 00:00:00

abstract：:Early region 1 (E1) of the human adenoviruses has many intriguing properties which have prompted numerous mutational studies to help delineate and characterize the domains responsible for these functions. In mutational analyses being done currently, the E1 region is usually cloned into a bacterial plasmid where it is ...

journal_title：Virology

authors： McGrory WJ,Bautista DS,Graham FL

更新日期：1988-04-01 00:00:00

abstract：:Molluscum contagiosum virus (MCV) is a human poxvirus that produces small, benign skin tumors primarily in young children and encodes proteins that modulate the host immune response. The MC51L, MC53L, and MC54L open reading frames of MCV have significant amino acid sequence similarities and related proteins are encode...

journal_title：Virology

authors： Xiang Y,Moss B

更新日期：1999-05-10 00:00:00

abstract：:Cucumber seedlings infected with cucumber mosaic virus (CMV) contain a virus-induced RNA-dependent RNA polymerase (RNA replicase) in both soluble and particulate fractions of plant extracts; no such activity is found in uninfected plants. The extensive purification and characterization of the soluble RNA replicase has...

journal_title：Virology

authors： Gill DS,Kumarasamy R,Symons RH

更新日期：1981-08-01 00:00:00

abstract：:Previous in vitro work demonstrated the incorporation of the major inducible 70k heat shock protein (i.e., 72k HSP) into the biologically active light nucleocapsid (L-NC) variant of canine distemper virus (CDV). Here, in vitro induction of the cellular stress response, characterized by elevated cytoplasmic and intranu...

journal_title：Virology

authors： Oglesbee MJ,Kenney H,Kenney T,Krakowka S

更新日期：1993-02-01 00:00:00

abstract：:BS-1, a stroma cell line derived from normal human bone marrow, can support the growth of murine erythroid (BFU-E), granulocyte-macrophage (CFU-GM), and megakaryocyte (CFU-Meg) progenitor cells in a short-term in vitro coculture system. Exposure of BS-1 cell to the human immunodeficiency virus (HIV) prior to coculture...

journal_title：Virology

authors： Steinberg HN,Anderson J,Crumpacker CS,Chatis PA

更新日期：1993-03-01 00:00:00

abstract：:Recent findings suggested that the SUMO-interacting motifs (SIMs) present in the human TRIM5α (TRIM5α(hu)) protein play an important role in the ability of TRIM5α(hu) to restrict N-MLV. Here we explored the role of SIMs in the ability of rhesus TRIM5α (TRIM5α(rh)) to restrict HIV-1, and found that TRIM5α(rh) SIM mutan...

journal_title：Virology

authors： Brandariz-Nuñez A,Roa A,Valle-Casuso JC,Biris N,Ivanov D,Diaz-Griffero F

更新日期：2013-01-20 00:00:00

abstract：:The bipartite geminiviruses bean golden mosaic virus (BGMV), cabbage leaf curl virus (CabLCV), and tomato golden mosaic virus (TGMV) exhibit differential tissue tropism in Nicotiana benthamiana. In systemically infected leaves, BGMV remains largely confined to vascular-associated cells (phloem-limited), whereas CabLCV...

journal_title：Virology

authors： Qin Y,Petty IT

更新日期：2001-12-20 00:00:00

abstract：:Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant p...

journal_title：Virology

authors： Bhrigu V,Trempe JP

更新日期：2009-07-20 00:00:00

abstract：:Marburg virus (MBGV), for which no vaccines or treatments currently exist, causes an acute hemorrhagic fever with a high mortality rate in humans. We previously showed that immunization with either killed MBGV or a glycoprotein (GP) subunit prevented lethal infection in guinea pigs. In the studies reported here, an RN...

journal_title：Virology

authors： Hevey M,Negley D,Pushko P,Smith J,Schmaljohn A

更新日期：1998-11-10 00:00:00

abstract：:The major late promoter (MLP) of the subgroup C human adenoviruses is a preeminent model for the study of the mechanisms of basal and activated transcription, both in vivo and in vitro. However, while the structure and function of the human virus MLP has been the subject of extensive investigation, the conservation of...

journal_title：Virology

authors： Song B,Hu SL,Darai G,Spindler KR,Young CS

更新日期：1996-06-15 00:00:00

abstract：:Mouse mammary tumour virus (MMTV) encodes a viral superantigen (Sag) and a negative acting factor (Naf) which share parts of their coding sequence. Using 2-dimensional gel electrophoresis (2D-DIGE), we could show that at least 10 different cellular proteins were differentially expressed in Naf positive cells. Also, lu...

journal_title：Virology

authors： Metzner C,Salmons B,Gunzburg WH,Gemeiner M,Miller I,Gesslbauer B,Kungl A,Dangerfield JA

更新日期：2006-03-01 00:00:00

abstract：:During respiratory syncytial virus (RSV) infection there is a close physical interaction between the filamentous actin (F-actin) and the virus, involving both inclusion bodies and the virus filaments. This interaction appears to occur relatively early in the replication cycle, and can be detected from 8 h post-infecti...

journal_title：Virology

authors： Jeffree CE,Brown G,Aitken J,Su-Yin DY,Tan BH,Sugrue RJ

更新日期：2007-12-20 00:00:00

abstract：:Here, we evaluated the role of two host proteins, a Bromo domain containing RNA binding protein (BRP1) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), in the replication of Cucumber mosaic virus (CMV). LC-MS/MS analysis of host/viral proteins pull down against BRP1 from CMV-infected plants co-infiltrated with BR...

journal_title：Virology

authors： Chaturvedi S,Seo JK,Rao AL

更新日期：2016-07-01 00:00:00

abstract：:We reported previously that carbohydrate attachment to an overlapping glycosylation site adjacent to the signal peptide of GP3 from equine arteritis virus (EAV) prevents cleavage. Here we investigated whether this unusual processing scheme is a feature of GP3s of other Arteriviridae, which all contain a glycosylation ...

journal_title：Virology

authors： Zhang M,Veit M

更新日期：2018-04-01 00:00:00

abstract：:Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a hi...

journal_title：Virology

authors： Zhang W,Bouwman KM,van Beurden SJ,Ordonez SR,van Eijk M,Haagsman HP,Verheije MH,Veldhuizen EJA

更新日期：2017-09-01 00:00:00

abstract：:Cauliflower mosaic virus gene VI product (P6) is an essential protein that forms cytoplasmic, inclusion bodies (IBs). P6 contains four regions involved in self-association, termed D1-D4. D3 binds to D1, along with D4 and contains a spacer region (termed D3b) between two RNA-binding domains. Here we show D3b binds full...

journal_title：Virology

authors： Lutz L,Okenka G,Schoelz J,Leisner S

更新日期：2015-02-01 00:00:00

abstract：:A mutant strain of polyoma virus encoding a truncated middle T protein has been studied for its ability to replicate and induce tumors following inoculation into newborn mice. Virus replication in the acute period prior to expected onset of tumors as well as persistence of virus in older animals were followed. The mut...

journal_title：Virology

authors： Freund R,Sotnikov A,Bronson RT,Benjamin TL

更新日期：1992-12-01 00:00:00

abstract：:This report presents a procedure for evaluating the relative affinities of simple sialoside receptor determinants for their interaction with the receptor binding pocket of the influenza virus hemagglutinin. The virus examined was A/Memphis/102/72, previously shown to exhibit preferential binding specificity for enzyma...

journal_title：Virology

authors： Pritchett TJ,Brossmer R,Rose U,Paulson JC

更新日期：1987-10-01 00:00:00

abstract：:The complete RNA sequence of Sabin 3 (LED3) used in vaccine in the United States has been determined. The LED3 Sabin 3 sequence contains the attenuating mutations at bases 472 and 2034 but differs from that published by Stanway et al. (Nucleic Acids Res., 11, 5629-5643, 1983) at two other base positions, 2493 and 6061...

journal_title：Virology

authors： Weeks-Levy C,Tatem JM,DiMichele SJ,Waterfield W,Georgiu AF,Mento SJ

更新日期：1991-12-01 00:00:00