Abstract:
:In order to study the effect of nef gene expression on viral replication in monocytic cells, we established monocytic (U937 and THP-1) cell transfectants constitutively expressing the human immunodeficiency virus type 1 nef gene. We constructed a plasmid expressing the nef gene derived from an infectious clone, NL432, under the control of SR alpha promoter which can drive a high level of gene expression. We found suppressed viral replication in nef-expressing monocytic cells, although a negative effect of nef was observed, with some variation depending on the virus strain and the cell. We also observed that the expression of the surface CD4 molecule is inversely related to the expression of the nef gene, especially in the U937 transfectants. These results indicate that the suppression of viral replication and the down-modulation of CD4 molecule by nef gene expression occur in monocytic cell lines as in T cell lines.
journal_name
Virologyjournal_title
Virologyauthors
Tsunetsugu-Yokota Y,Matsuda S,Maekawa M,Saito T,Takemori T,Takebe Ydoi
10.1016/0042-6822(92)90272-qsubject
Has Abstractpub_date
1992-12-01 00:00:00pages
960-3issue
2eissn
0042-6822issn
1096-0341journal_volume
191pub_type
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