Biologically active protease 3C of human rhinovirus 1A is expressed from a cloned cDNA segment in Escherichia coli.

abstract：:A virus was found to be associated with a severe disease affecting rabbits on a farm near Anchorage, Alaska. Extracts from the skin of infected rabbits produced syncytia and cell lysis in cultured rabbit skin, rabbit kidney, and Vero cells. Examination of the infectious agent by electron microscopy revealed an icosahe...

journal_title：Virology

authors： Jin L,Löhr CV,Vanarsdall AL,Baker RJ,Moerdyk-Schauwecker M,Levine C,Gerlach RF,Cohen SA,Alvarado DE,Rohrmann GF

更新日期：2008-08-15 00:00:00

abstract：:The herpes simplex virus single-stranded DNA-binding protein, ICP8, localizes initially to structures in the nucleus called prereplicative sites. As replication proceeds, these sites mature into large globular structures called replication compartments. The details of what signals or proteins are involved in the redis...

journal_title：Virology

authors： Taylor TJ,Knipe DM

更新日期：2003-05-10 00:00:00

abstract：:The propensity of retroviruses to rapidly establish persistent infections poses a formidable problem in vaccination strategies. In the current study, we use a live attenuated vaccine to study protection against acute and persistent Friend virus infections in mice. Adoptive transfers of immune CD8(+) T cells combined w...

journal_title：Virology

authors： Dittmer U,Hasenkrug KJ

更新日期：2000-06-20 00:00:00

abstract：:We have previously showed that equine arteritis virus (EAV), an arterivirus, induces apoptosis in vitro. To determine the caspase activation pathways involved in EAV-induced apoptosis, target cells were treated with peptide inhibitors of apoptosis Z-VAD-FMK (pan-caspase inhibitor), Z-IETD-FMK (caspase-8-specific inhib...

journal_title：Virology

authors： St-Louis MC,Archambault D

更新日期：2007-10-10 00:00:00

abstract：:Human cytomegalovirus (HCMV) lytic DNA replication is initiated at the cis-acting oriLyt region and requires six core replication proteins along with UL84 and IE2. Although UL84 is thought to be the replication initiator protein, little is known about its interaction with oriLyt. We have now performed chromatin immuno...

journal_title：Virology

authors： Kagele D,Gao Y,Smallenburg K,Pari GS

更新日期：2009-09-15 00:00:00

abstract：:The envelope glycoprotein E2 of hepatitis C virus (HCV) is a major component of the viral envelope. Knowledge of its topologic features and antigenic determinants in virions is crucial in understanding the viral binding sites to cellular receptor(s) and the induction of neutralizing antibodies. The lack of a robust ce...

journal_title：Virology

authors： Triyatni M,Vergalla J,Davis AR,Hadlock KG,Foung SK,Liang TJ

更新日期：2002-06-20 00:00:00

abstract：:The complete genomic RNAs of two Korean sacbrood virus (SBV) strains, which infect the honey bee, Apis mellifera, were sequenced. The two sequences (AmSBV-Kor19, AmSBV-Kor21) were distinguished by the presence or absence of a PstI restriction site. These strains comprised of 8784 bp and 8835 bp; contained a single lar...

journal_title：Virology

authors： Choe SE,Nguyen LT,Noh JH,Kweon CH,Reddy KE,Koh HB,Chang KY,Kang SW

更新日期：2012-10-10 00:00:00

abstract：:RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...

journal_title：Virology

authors： Navarro JA,Darós JA,Flores R

更新日期：1999-01-05 00:00:00

abstract：:Cell lines persistently infected with foot-and-mouth disease virus (FMDV) have been established by growth of BHK-21 (c-13) or IBRS-2 (c-26) that survived standard cytolytic infections with FMDV. They maintain cytoplasmic FMDV RNA sequences, as shown by dot blot hybridization tests, using cloned FMDV cDNA as probes. Ce...

journal_title：Virology

authors： de la Torre JC,Dávila M,Sobrino F,Ortín J,Domingo E

更新日期：1985-08-01 00:00:00

abstract：:The hemagglutinin of the 2009 pandemic H1N1 influenza virus is a derivative of and is antigenically related to classical swine but not to seasonal human H1N1 viruses. We compared the A/California/7/2009 (CA/7/09) virus recommended by the WHO as the reference virus for vaccine development, with two classical swine infl...

journal_title：Virology

authors： Min JY,Chen GL,Santos C,Lamirande EW,Matsuoka Y,Subbarao K

更新日期：2010-12-05 00:00:00

abstract：:HIV CRF07 B'/C is a strain circulating mainly in northwest region of China. The gp41 region of CRF07 is derived from a clade C virus. In order to compare the difference of CRF07 gp41 with that of typical clade B virus, we solved the crystal structure of the core region of CRF07 gp41. Compared with clade B gp41, CRF07 ...

journal_title：Virology

authors： Du J,Xue H,Ma J,Liu F,Zhou J,Shao Y,Qiao W,Liu X

更新日期：2013-11-01 00:00:00

abstract：:Chikungunya virus (CHIKV) infection leads to activation of innate immune response by triggering Toll-like receptor (TLR) pathways resulting in elevated cytokines and type-I interferon levels. Genetic variations of these genes may influence human CHIKV-susceptibility and disease progression. Present study aimed to iden...

journal_title：Virology

authors： Dutta SK,Tripathi A

更新日期：2017-11-01 00:00:00

abstract：:Vpr is one of the auxiliary gene products encoded by HIV-1 genome. Vpr is a 14-kDa protein and exhibits several interesting characteristics including incorporation into virus particles, oligomerization, localization in the nucleus, and positive regulation of virus replication in primary cells. In an effort to define t...

journal_title：Virology

authors： Mahalingam S,Patel M,Collman RG,Srinivasan A

更新日期：1995-12-20 00:00:00

abstract：:The HIV-1 transframe protein p6 known to modulate HIV-1 protease activation has been suggested to interact with the viral pathogenicity factor Nef. However, a potential interaction site in p6 has not been mapped so far. To evaluate effects of p6 modification on viral replication in light of Nef function, clustered sub...

journal_title：Virology

authors： Leiherer A,Ludwig C,Wagner R

更新日期：2009-04-25 00:00:00

abstract：:To study the role of human papillomavirus (HPV) infection in the development of genetic instability, we transduced normal human airway and anogenital epithelial cells with various combinations of HPV-16 E6, E7, and the reverse transcriptase component of telomerase (hTERT). Cell lines generated by co-expression of E7 w...

journal_title：Virology

authors： Klingelhutz AJ,Qian Q,Phillips SL,Gourronc FA,Darbro BW,Patil SR

更新日期：2005-09-30 00:00:00

abstract：:The antibody response in serum and the reproductive tract of female rabbits to a model antigen, influenza virus haemagglutinin (HA), encoded by a recombinant myxoma virus was investigated. Strong and lasting IgG antibody responses to HA were induced in serum following intradermal, intranasal, and intravaginal immunisa...

journal_title：Virology

authors： Gu W,Holland M,Janssens P,Kerr P

更新日期：2003-08-15 00:00:00

abstract：:Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic li...

journal_title：Virology

authors： Kulkarni SS,Lapedes A,Tang H,Gnanakaran S,Daniels MG,Zhang M,Bhattacharya T,Li M,Polonis VR,McCutchan FE,Morris L,Ellenberger D,Butera ST,Bollinger RC,Korber BT,Paranjape RS,Montefiori DC

更新日期：2009-03-15 00:00:00

abstract：:The nucleotide and deduced amino acid sequence of G serotype 3 equine rotavirus strain H-2 was determined. A predicted 776-amino-acid H-2 VP4 shows less than or equal to 85.3% identity to other rotavirus VP4 types sequenced to date and thus represents a new P serotype. A PCR-generated probe derived from a cDNA clone o...

journal_title：Virology

authors： Hardy ME,Gorziglia M,Woode GN

更新日期：1993-03-01 00:00:00

abstract：:A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome se...

journal_title：Virology

authors： Qiu WP,Geske SM,Hickey CM,Moyer JW

更新日期：1998-04-25 00:00:00

abstract：:Sindbis virus (SINV) induces inflammatory and vasoactive responses that are associated with rash and arthritis in human infections. The mechanisms underlying infection-associated microvasculopathy are still unknown. We investigated whether endothelial cells infected by SINV are differentially responsive to bradykinin ...

journal_title：Virology

authors： Rust NM,Papa MP,Scovino AM,da Silva MM,Calzavara-Silva CE,Marques ET Jr,Peçanha LM,Scharfstein J,Arruda LB

更新日期：2012-01-05 00:00:00

abstract：:Piglets infected in utero with porcine reproductive and respiratory syndrome virus (PRRSV) are born severely immunocompromised. In this article we more closely examine the effects of in utero PRRSV infection on circulating and thymic T cell populations. Numbers of CD4+, CD8+, and dual-positive lymphocytes were quantit...

journal_title：Virology

authors： Feng WH,Tompkins MB,Xu JS,Brown TT,Laster SM,Zhang HX,McCaw MB

更新日期：2002-10-25 00:00:00

abstract：:The longest hepatitis B virus transcript is a 3.9-kb mRNA whose function remained unclear. In this study, we wished to identify the translation products and physiological role of this viral transcript. This transcript initiates from the X promoter region ignoring the inefficient and noncanonical viral polyadenylation ...

journal_title：Virology

authors： Doitsh G,Shaul Y

更新日期：2003-05-10 00:00:00

abstract：:Segment 7 (M) of the cold-adapted live influenza A virus vaccine plays a primary role in the ability of this virus to interfere with the replication of wild-type influenza A viruses. This conclusion is based on several lines of evidence. Single gene reassortant viruses derived by crossing influenza A/Ann Arbor/6/60 (H...

journal_title：Virology

authors： Whitaker-Dowling P,Zvolenski R,Youngner JS

更新日期：1991-01-01 00:00:00

abstract：:Next generation sequence analyses were used to assess virus-derived small RNA (vsRNA) profiles for Homalodisca coagulata virus-1 (HoCV-1), family Dicistroviridae, and Homalodisca vitripennis reovirus (HoVRV), family Reoviridae, from virus-infected H. vitripennis, the glassy-winged sharpshooter. The vsRNA reads were ma...

journal_title：Virology

authors： Nandety RS,Fofanov VY,Koshinsky H,Stenger DC,Falk BW

更新日期：2013-07-20 00:00:00

abstract：:The 5' leader region of the HIV-1 RNA contains the major 5' splice site (ss) that is used in the production of all spliced viral RNAs. This splice-donor (SD) region can fold a stem-loop structure. We demonstrate that whereas stabilization of this SD hairpin reduces splicing efficiency, destabilization increases splici...

journal_title：Virology

authors： Mueller N,van Bel N,Berkhout B,Das AT

更新日期：2014-11-01 00:00:00

abstract：:Picornavirus RNA translation is driven by the internal ribosome entry site (IRES) element. The impact of RNA structure on the foot-and-mouth disease virus (FMDV) IRES activity has been analyzed using Selective 2'Hydroxyl Acylation analyzed by Primer Extension (SHAPE) and high throughput analysis of RNA conformation by...

journal_title：Virology

authors： Fernández N,García-Sacristán A,Ramajo J,Briones C,Martínez-Salas E

更新日期：2011-01-20 00:00:00

abstract：:Molluscum contagiosum virus (MCV) is a human poxvirus that produces small, benign skin tumors primarily in young children and encodes proteins that modulate the host immune response. The MC51L, MC53L, and MC54L open reading frames of MCV have significant amino acid sequence similarities and related proteins are encode...

journal_title：Virology

authors： Xiang Y,Moss B

更新日期：1999-05-10 00:00:00

abstract：:Infection of different human epithelial cell lines with human respiratory syncytial virus (HRSV) revealed significant differences in the electrophoretic mobility of the viral attachment glycoprotein (G). Cell-type specific differences in G protein glycosylation were observed with certain lectins and sugar-specific rea...

journal_title：Virology

authors： García-Beato R,Martínez I,Francí C,Real FX,García-Barreno B,Melero JA

更新日期：1996-07-15 00:00:00

abstract：:Viroids are small, nontranslatable pathogenic RNAs that replicate autonomously and traffic systemically in their host plants. We have used in situ hybridization to analyze the trafficking pattern of Potato spindle tuber viroid (PSTVd) in tomato and Nicotiana benthamiana. When PSTVd was inoculated onto the stem of a pl...

journal_title：Virology

authors： Zhu Y,Green L,Woo YM,Owens R,Ding B

更新日期：2001-01-05 00:00:00

abstract：:The terminal sequences of both genomic dsRNA segments of different strains of infectious bursal disease virus were determined. Analysis of the linear sequences revealed in the 5'-noncoding region of both segments the presence of a 32-nucleotide sequence with motifs conserved between segments and serotypes. The 3'-nonc...

journal_title：Virology

authors： Mundt E,Müller H

更新日期：1995-05-10 00:00:00