Abstract:
:To gain new insights regarding the role of Ku, the DNA-PK DNA-binding component, during lentiviral DNA integration, we have investigated the HIV-1 replication in Ku80-depleted human cells. CEM4fx cells underexpressing the Ku80 factor were selected after transduction by a retroviral vector expressing the Ku80 full-length antisense sequence. De novo infection experiment with NL4.3 HIV-1 strain led to the observation that the viral replication was delayed in the Ku80-depleted cells. Early events of the replicative cycle, including nuclear import of the viral DNA, were not affected. In contrast, the formation of the 2-LTR circles was impaired, thus demonstrating the implication of Ku in HIV-1 DNA circularization, for the first time in human cells. Furthermore, the detection of integrated proviruses by an Alu-LTR-nested PCR amplification method was affected in cells underexpressing Ku80. These results suggest that this factor may also be involved in the mechanisms leading to the stable establishment of HIV-1 provirus.
journal_name
Virologyjournal_title
Virologyauthors
Jeanson L,Subra F,Vaganay S,Hervy M,Marangoni E,Bourhis J,Mouscadet JFdoi
10.1006/viro.2002.1515subject
Has Abstractpub_date
2002-08-15 00:00:00pages
100-8issue
1eissn
0042-6822issn
1096-0341pii
S0042682202915151journal_volume
300pub_type
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