Abstract:
:The human hepatitis B Virus genome (HBV) contains a liver-specific enhancer upstream of the X ORF which has been studied in detail by several investigators. A second liver-specific enhancer element, designated here as enhancer II, has been relatively recently described in the HBV genome, which is located within the core/pregenomic promoter. We have studied the interactions of transcriptional factors with this element and show here that the nuclear factor CCAAT/enhancer binding protein (C/EBP) binds at a unique site within these sequences. Further, using the transient cotransfection scheme of expression with C/EBP encoding vectors and an enhancer II-reporter gene construct, we demonstrate that the enhancer element II responds to increasing amounts of C/EBP by displaying transactivation. Evidence for the functional role of the enhancer element II in transcriptional regulation of the HBV gene expression is presented. A major influence of the enhancer II appears to be on the surface antigen expression.
journal_name
Virologyjournal_title
Virologyauthors
López-Cabrera M,Letovsky J,Hu KQ,Siddiqui Adoi
10.1016/0042-6822(91)91019-dsubject
Has Abstractpub_date
1991-08-01 00:00:00pages
825-9issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(91)91019-Djournal_volume
183pub_type
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