Mapping of the tobacco vein mottling virus VPg cistron.

abstract：:The amino acid sequence of the coat protein of wild-type cowpea chlorotic mottle virus has been nearly completely determined by direct methods. The sites of amino acid replacements were identified in the coat proteins of an oxidation-sensitive mutant, a temperature-sensitive mutant, and a salt-stable mutant. The repla...

journal_title：Virology

authors： Rees MW,Short MN

更新日期：1982-06-01 00:00:00

abstract：:Previous studies have shown that the transmembrane (TM) domain of the subtype B Vpu enhances virion release from cells and some studies have shown that this domain may form an oligomeric structure with properties of an ion channel. To date, no studies have been performed to assess the role of this domain in virus path...

journal_title：Virology

authors： Hout DR,Gomez ML,Pacyniak E,Gomez LM,Inbody SH,Mulcahy ER,Culley N,Pinson DM,Powers MF,Wong SW,Stephens EB

更新日期：2005-08-15 00:00:00

abstract：:In benign squamous lesions and in organotypic epithelial cultures, the human papillomavirus (HPV) E6 and E7 genes are transcriptionally up-regulated in differentiated, spinous keratinocytes. We previously identified sequence elements in the enhancer-promoter regions of HPV types 18 and 11 important for this promoter r...

journal_title：Virology

authors： Zhao W,Chow LT,Broker TR

更新日期：1999-01-20 00:00:00

abstract：:Crosslinking reagents were used to interrupt the process of filamentous phage morphogenesis and investigate the orientation in which nascent virions are extruded through the host cell membrane. Infected bacteria with emerging phage particles were crosslinked with glutaraldehyde. Immunoferritin-labeling studies on thes...

journal_title：Virology

authors： Lopez J,Webster RE

更新日期：1983-05-01 00:00:00

abstract：:The influenza RNA polymerase is known to catalyse three distinct copying activities: (i) transcription of minus-sense virion RNA (vRNA) into mRNA, (ii) transcription of vRNA into full-length complementary RNA (cRNA), and (iii) transcription of cRNA to vRNA. Ever since the discovery of the conserved 13 and 12 long sequ...

journal_title：Virology

authors： Seong BL,Brownlee GG

更新日期：1992-01-01 00:00:00

abstract：:Adenovirus-mediated virotherapy is one of the promising therapeutic approaches for glioma treatment. However, its replication efficiency and specificity still failed to meet the requirements for clinical treatment. To improve the anti-tumor activity and specificity of oncolytic adenoviruses (OA), we applied multiple m...

journal_title：Virology

authors： Yao W,Guo G,Zhang Q,Fan L,Wu N,Bo Y

更新日期：2014-06-01 00:00:00

abstract：:Herpes simplex virus 1 (HSV-1) glycoprotein B (gB) is a multifunctional glycoprotein required for infectivity; it is thought to promote fusion of the viral envelope with the cell membrane and entry of virions into cells. To map the antigenic and functional domains on gB, we constructed amino terminal derivatives lacki...

journal_title：Virology

authors： Pereira L,Ali M,Kousoulas K,Huo B,Banks T

更新日期：1989-09-01 00:00:00

abstract：:Alpha human papillomaviruses (HPVs) are among the most common sexually transmitted agents of which a subset causes cervical neoplasia and cancer in humans. Alpha-PVs have also been identified in non-human primates although few studies have systematically characterized such types. We cloned and characterized 10 distinc...

journal_title：Virology

authors： Chen Z,van Doorslaer K,DeSalle R,Wood CE,Kaplan JR,Wagner JD,Burk RD

更新日期：2009-10-25 00:00:00

abstract：:Specific binding sites for Epstein-Barr virus (EBV) nuclear antigen (EBNA-1), isolated and semipurified from EBV-transformed nonproductive Raji cells, were visualized on the molecule of EBV DNA by electron microscopy and mapped. Two measures had to be applied to counteract the limited purity of the EBNA-1 preparation:...

journal_title：Virology

authors： Reischig J,Bartsch D,Polack A,Vonka V,Hirsch I

更新日期：1987-10-01 00:00:00

abstract：:The genome of the Bacillus subtilis phage SP01 contains a 12.6-kilobase-pair terminal redundancy. The transcription units in this region, which are utilized in vitro by B. subtilis RNA polymerase holoenzme, have been mapped. In vitro transcripts were separated and characterized by gel electrophoresis. Since there is s...

journal_title：Virology

authors： Brennan SM,Chelm BK,Romeo JM,Geiduschek EP

更新日期：1981-06-01 00:00:00

abstract：:The genus Badnavirus is characterized by members that are genetically and serologically heterogeneous which presents challenges for their detection and characterization. The presence of integrated badnavirus-like sequences in some host species further complicates detection using PCR-based protocols. To address these c...

journal_title：Virology

authors： Sukal AC,Kidanemariam DB,Dale JL,Harding RM,James AP

更新日期：2019-03-01 00:00:00

abstract：:Prions were purified from scrapie-infected hamster brains and incubated for 24 hr at 65 degrees with 2 mM Zn2+ or 5 mM Mg2+; no loss of infectivity was observed. Bacteriophage M13, tobacco mosaic virus (TMV), potato virus X, and potato spindle tuber viroid were all inactivated by divalent metal ions under these condit...

journal_title：Virology

authors： Bellinger-Kawahara C,Diener TO,McKinley MP,Groth DF,Smith DR,Prusiner SB

更新日期：1987-09-01 00:00:00

abstract：:When constructing viruses that have desired hybrid phenotypes, anticipated difficulties include the nonviability of many, possibly most, of the hybrid genomes that can be constructed by incorporation of DNA fragments. Therefore, many different hybrid genomes may have to be constructed in order to find one that is viab...

journal_title：Virology

authors： Khan SA,Watson RH,Hayes SJ,Serwer P

更新日期：1997-01-20 00:00:00

abstract：:Our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. In the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express CD8alpha and TCRbeta...

journal_title：Virology

authors： Somamoto T,Yoshiura Y,Sato A,Nakao M,Nakanishi T,Okamoto N,Ototake M

更新日期：2006-05-10 00:00:00

abstract：:Corn lethal necrosis (CLN) is caused by the synergistic interaction between maize chlorotic mottle machlomovirus (MCMV) and any potyvirus which infects cereals. Interactions between MCMV and wheat streak mosaic rymovirus (WSMV) in N28Ht corn produced MCMV concentrations that averaged 3.3- to 11.2-fold higher in doubly...

journal_title：Virology

authors： Scheets K

更新日期：1998-03-01 00:00:00

abstract：:Bovine herpesvirus 5 (BHV-5) is a neurovirulent alpha-herpesvirus that causes fatal encephalitis in calves. We previously demonstrated that deletion of a glycine-rich epitope in the gE ectodomain dramatically reduced BHV-5 neurovirulence. To investigate the role of gE cytoplasmic tail sequences in the neuropathogenesi...

journal_title：Virology

authors： Al-Mubarak A,Simon J,Coats C,Okemba JD,Burton MD,Chowdhury SI

更新日期：2007-09-01 00:00:00

abstract：:To investigate the role of Tobacco vein banding mosaic virus (TVBMV) 3'-UTR in virus systemic infection, three types of deletions were introduced into TVBMV infectious clone pCaTVBMV-GFP. Mutants with deletions at the nucleotide position 8-42, 43-141, or 163-174 in the 3'-UTR failed to cause systemic infection in N. b...

journal_title：Virology

authors： Wang Y,Yang ZY,Tian YP,Geng C,Yuan XF,Li XD

更新日期：2019-01-15 00:00:00

abstract：:Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discov...

journal_title：Virology

authors： Tu H,Gao L,Shi ST,Taylor DR,Yang T,Mircheff AK,Wen Y,Gorbalenya AE,Hwang SB,Lai MM

更新日期：1999-10-10 00:00:00

abstract：:The objective of this study was to identify porcine reproductive and respiratory syndrome virus (PRRSV)-encoded proteins that are responsible for the inhibition of TNF-α expression and the mechanism(s) involved in this phenomenon. Using a TNF-α promoter reporter system, the non-structural protein 1 (Nsp1) was found to...

journal_title：Virology

authors： Subramaniam S,Kwon B,Beura LK,Kuszynski CA,Pattnaik AK,Osorio FA

更新日期：2010-10-25 00:00:00

abstract：:The dependence of retroviral replication on cell proliferation was described as early as 1958, although different classes of retroviruses are able to infect non-dividing cells with different efficiencies. For example, the human immunodeficiency virus (HIV) and other lentiviruses infect most non-dividing cells nearly a...

journal_title：Virology

authors： Yamashita M,Emerman M

更新日期：2006-01-05 00:00:00

abstract：:Retrons are unusual, reverse transcriptase-encoding elements found in bacteria. Although there are a number of indications that retrons are mobile elements, their transposition has not been observed. The Escherichia coli retrons Ec67 and Ec86 are different retrons inserted at the same site and we have further characte...

journal_title：Virology

authors： Dodd IB,Egan JB

更新日期：1996-05-01 00:00:00

abstract：:We have examined the host range of AIDS-associated retroviruses (ARV) that are known to infect human T cells of the helper subset. We have observed that the virus cannot infect fibroblast and epithelial cell lines of many different animal species. It is infectious and replicates efficiently in peripheral mononuclear c...

journal_title：Virology

authors： Levy JA,Shimabukuro J,McHugh T,Casavant C,Stites D,Oshiro L

更新日期：1985-12-01 00:00:00

abstract：:To gain new insights regarding the role of Ku, the DNA-PK DNA-binding component, during lentiviral DNA integration, we have investigated the HIV-1 replication in Ku80-depleted human cells. CEM4fx cells underexpressing the Ku80 factor were selected after transduction by a retroviral vector expressing the Ku80 full-leng...

journal_title：Virology

authors： Jeanson L,Subra F,Vaganay S,Hervy M,Marangoni E,Bourhis J,Mouscadet JF

更新日期：2002-08-15 00:00:00

abstract：:A novel virus isolated from the feather follicles of cockatoos diagnosed as having psittacine beak and feather disease was characterized by electron microscopy, nucleic acid content, and polypeptide composition. Purified virions displayed an icosahedral symmetry, were nonenveloped, and had a mean diameter of 14 to 16 ...

journal_title：Virology

authors： Ritchie BW,Niagro FD,Lukert PD,Steffens WL 3rd,Latimer KS

更新日期：1989-07-01 00:00:00

abstract：:To examine whether there are HBV variants not yet described which cannot express HBe due to a mutation in the pre-C region, and, if they exist, whether they cause a particular course of infection and disease, we analyzed the HBV genomes of a HBs/anti-HBe positive chronic carrier who had several episodes of acute reexa...

journal_title：Virology

authors： Raimondo G,Schneider R,Stemler M,Smedile V,Rodino G,Will H

更新日期：1990-11-01 00:00:00

abstract：:The prevalence of hepatitis B virus vaccine escape mutants has increased as a consequence of the introduction of global vaccination programs. Furthermore and as a consequence of the organization of the genome of hepatitis B virus (HBV) into overlapping reading frames, the selection of polymerase mutants during long-te...

journal_title：Virology

authors： Torresi J,Earnest-Silveira L,Deliyannis G,Edgtton K,Zhuang H,Locarnini SA,Fyfe J,Sozzi T,Jackson DC

更新日期：2002-02-15 00:00:00

abstract：:Recombination is a driving force for the emergence, evolution and virulence/epidemics of viruses, comprising the Enterovirus genus of the Picornaviridae family, important for human and animal health. By analyzing 2949 complete genomes/coding sequences, we provide a thorough and up-to-date overview of the genome-wide p...

journal_title：Virology

authors： Nikolaidis M,Mimouli K,Kyriakopoulou Z,Tsimpidis M,Tsakogiannis D,Markoulatos P,Amoutzias GD

更新日期：2019-01-02 00:00:00

abstract：:The function of a substantial percentage of the putative protein-coding open reading frames (ORFs) in viral genomes is unknown. As their sequence is not similar to that of proteins of known function, the function of these ORFs cannot be assigned on the basis of sequence similarity. Methods complement or in combination...

journal_title：Virology

authors： Han LY,Cai CZ,Ji ZL,Chen YZ

更新日期：2005-01-05 00:00:00

abstract：:Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-1 infection. In the pre...

journal_title：Virology

authors： Bacot SM,Feldman GM,Yamada KM,Dhawan S

更新日期：2015-02-01 00:00:00

abstract：:Segmented genomes of RNA viruses are thought to evolve and be maintained in analogy to sexual recombination and reassortment in eukaryotic systems. If reassortment among genomes is an important event in cucumoviral evolution, then such events should be detectable among extant viruses. In this study, phylogenetic analy...

journal_title：Virology

authors： White PS,Morales F,Roossinck MJ

更新日期：1995-02-20 00:00:00