Abstract:
:The genus Badnavirus is characterized by members that are genetically and serologically heterogeneous which presents challenges for their detection and characterization. The presence of integrated badnavirus-like sequences in some host species further complicates detection using PCR-based protocols. To address these challenges, we have assessed and optimized various RCA protocols including random-primed RCA (RP-RCA), primer-spiked random-primed RCA (primer-spiked RP-RCA), directed RCA (D-RCA) and specific-primed RCA (SP-RCA). Using Dioscorea bacilliform AL virus (DBALV) as an example, we demonstrate that viral DNA amplified using the optimized D-RCA and SP-RCA protocols showed an 85-fold increase in badnavirus NGS reads compared with RP-RCA. The optimized RCA techniques described here were used to detect a range of badnaviruses infecting banana, sugar cane, taro and yam demonstrating the utility of RCA for detection of diverse badnaviruses infecting a variety of host plant species.
journal_name
Virologyjournal_title
Virologyauthors
Sukal AC,Kidanemariam DB,Dale JL,Harding RM,James APdoi
10.1016/j.virol.2019.01.013subject
Has Abstractpub_date
2019-03-01 00:00:00pages
73-80eissn
0042-6822issn
1096-0341pii
S0042-6822(19)30013-3journal_volume
529pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The bottom component B1a of TYMV with a buoyant density of 1.42-1.43 g/ml, is converted to a more dense particle B2a following CsCI density gradient centrifugation. This conversion is complete after a prior incubation of the virus in the CsCl solution at elevated temperatures. It is shown that the formation of B28 is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90041-1
更新日期:1982-07-30 00:00:00
abstract::By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and pa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91205-9
更新日期:1992-09-01 00:00:00
abstract::Langat (LGT) flavivirus, derived from infectious full-length cDNA clone 636, was investigated for its apoptotic activities in mouse neuroblastoma (Neuro-2a) and simian kidney (Vero and LLC-MK(2)) cells. The hallmark of apoptosis, cleavage of cellular DNA, was observed 48 h after infection of Vero, LLC-MK(2), and Neuro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0980
更新日期:2001-08-01 00:00:00
abstract::In order to molecularly characterize rapidly and slowly replicating HIV-1 variants, molecular clones were obtained from a rapid/high virus isolate. This isolate, 4803, had only been passaged in peripheral blood mononuclear cells (PBMC) prior to cloning. Molecular cloning was done in bacteriophage lambda-dash using hig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90469-r
更新日期:1991-03-01 00:00:00
abstract::The molecular host response to hepatitis C virus (HCV) infection was examined by isolation of HCV-induced genes from a cDNA library constructed from chimpanzee liver during the acute phase of hepatitis C. Two cDNA clones, 130-7 and 130-51, were obtained by differential hybridization with cDNA probes prepared from poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90925-f
更新日期:1992-10-01 00:00:00
abstract::Lloviu virus (LLOV), a phylogenetically divergent filovirus, is the proposed etiologic agent of die-offs of Schreibers's long-fingered bats (Miniopterus schreibersii) in western Europe. Studies of LLOV remain limited because the infectious agent has not yet been isolated. Here, we generated a recombinant vesicular sto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.08.019
更新日期:2014-11-01 00:00:00
abstract::Human immunodeficiency virus (HIV) encodes a transacting protein, Rev, which interacts with an RNA element (RRE) to mediate nuclear export of unspliced viral mRNA. Recently, the RNA constitutive transport elements (CTE) from Mason-Pfizer monkey virus (MPMV) and simian retrovirus type I (SRV-1) were shown to render Rev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8407
更新日期:1997-02-17 00:00:00
abstract::The biological phenotype of HIV-1 isolates from 21 subjects with primary HIV-1 infection was determined in primary lymphocytes and monocyte-derived macrophages as well as in three T-cell lines (MT-2, HUT78, and C8166). Seven isolates (33%) replicated on at least one cell line and were classified as rapid/high (n = 6) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1534
更新日期:1994-10-01 00:00:00
abstract::Infectious bronchitis has remained one of the most difficult to control diseases in poultry since it was first described in 1931. Previous studies demonstrated that primary CD8(+) T lymphocytes collected at 10 days post-infection (p.i.) are important in controlling acute infection. To further investigate the role of m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00059-4
更新日期:2003-02-15 00:00:00
abstract::The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90155-5
更新日期:1991-07-01 00:00:00
abstract::We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90491-6
更新日期:1988-02-01 00:00:00
abstract::The major capsid protein of HPV, L1, assembles into pentamers that form a T = 7 icosahedral particle, but the location of the co-assembled minor capsid protein, L2, remains controversial. Several researchers have developed useful monoclonal antibodies targeting L2, but most react with linear epitopes toward the N-term...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.08.017
更新日期:2018-11-01 00:00:00
abstract::The cellular protein MDM2 can bind to the tumor suppressor gene product p53 and abrogate its transcriptional activity. In addition, p53 can regulate expression of the mdm2 gene. We and others have previously shown that p53 is present at high levels in adenovirus-transformed cells which express the larger E1B protein. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1349
更新日期:1995-07-10 00:00:00
abstract::In a plantar cyst composed of the wall of the squamous cell layer and the horny inner substance in the lower dermis, we found characteristic pathological changes, such as cytoplasmic eosinophilic inclusions and vacuolated structure, and, immunohistochemically, the papillomavirus capsid antigen. The human papillomaviru...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91254-r
更新日期:1992-09-01 00:00:00
abstract::The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various role...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.023
更新日期:2018-02-01 00:00:00
abstract::Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90047-f
更新日期:1991-01-01 00:00:00
abstract::Two noncomplementing vaccinia virus temperature-sensitive mutants, Cts8 and Cts26, were mapped to the A3L gene, which encodes the major virion structural protein, 4b. The two ts mutants display normal patterns of gene expression, DNA replication, telomere resolution, and protein processing during infection. Morphogene...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.08.038
更新日期:2004-12-05 00:00:00
abstract::To elucidate the virulence factors of equine herpesvirus-1 (EHV-1), we compared the genomic architecture of the HH1 strain, a Japanese isolate of EHV-1, with that of its four descendant strains obtained by serial passage in bovine kidney (BK) cells, i.e. BK77, BK161, BK271, and BK343. In preliminary studies high-passa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1228
更新日期:1994-05-01 00:00:00
abstract::The influenza A virus nucleoprotein previously expressed in Escherichia coli after fusion to 32 heterologous amino acids has now been purified and tested for its ability to form complexes with RNA in vitro. By using a simple filter binding assay, we show that ribonucleoprotein (RNP) complexes form readily with single-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90419-3
更新日期:1987-02-01 00:00:00
abstract::The tegument protein pp28 of human cytomegalovirus (HCMV) is essential for the assembly of infectious HCMV virions, but how it functions during the process of HCMV tegumentation and envelopment remains unclear. By using live cell fluorescence resonance energy transfer (FRET) microscopy and yeast two-hybrid assays, we ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.007
更新日期:2009-05-25 00:00:00
abstract::Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, heparin) and copolymers of acrylic acid with vinylalcohol sulfate have proved to be potent inhibitors of human cytomegalovirus (CMV) infectivity in vitro. Sulfated alpha-cyclodextrins are only weak inhibitors of CMV. A close correlation was found...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90680-n
更新日期:1992-07-01 00:00:00
abstract::Genome-length cDNA clones of turnip crinkle virus (TCV) were constructed with SmaI and XbaI restriction sites engineered at the 5' and 3' termini, respectively. The genome-length cDNAs were positioned downstream of modified lambda and T7 phage promoters such that in vitro transcription resulted in RNAs with 5 extra nu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90368-1
更新日期:1989-05-01 00:00:00
abstract::The yellow fever 17D virus is attenuated and used for human vaccination. Two of its substrains, 17D-204 and 17DD, are used for vaccine production. One of the remarkable properties of this vaccine is limited viral replication in the host but with significant dissemination of the viral mass, yielding a robust and long-l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00583-x
更新日期:2003-11-10 00:00:00
abstract::The transmembrane envelope (TM) proteins of retroviruses are used as antigen in diagnostic immunoassays and they represent a conserved target for neutralizing antibodies. To analyze the situation in infections with the feline foamy virus (FFV), its recombinant TM protein was produced and used for ELISA and Western blo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.01.023
更新日期:2011-04-10 00:00:00
abstract::DNA sequence and transcriptional analyses were performed on the region of the equine herpesvirus type 1 (EHV-1) genome (KyA strain) (map units 0.129 to 0.152) encoding open reading frames (ORFs) 15 and 16. ORF16 encodes a homolog of glycoprotein C of HSV-1 (herpes simplex virus type 1), while ORF15 corresponds in posi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1200
更新日期:1993-04-01 00:00:00
abstract::All tissue culture systems for propagating HBV employed so far make use of tandemly arranged HBV genomes usually under the control of strong foreign promoters. Thus these systems are helpful for virus production but are of limited value in the investigation of the regulation of HBV replication or of the extent to whic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0049
更新日期:1996-02-01 00:00:00
abstract::Although rodents are important reservoirs for RNA viruses, to date only one species of rodent coronavirus (CoV) has been identified. Herein, we describe a new CoV, denoted Lucheng Rn rat coronavirus (LRNV), and novel variants of two Betacoronavirus species termed Longquan Aa mouse coronavirus (LAMV) and Longquan Rl ra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.10.017
更新日期:2015-01-01 00:00:00
abstract::Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0638
更新日期:2000-12-05 00:00:00
abstract::Maturation of T4 can proceed in the presence of 5-methyltryptophan or puromycin. Puromycin inhibits phage protein synthesis within a few seconds, thus limiting the amount of phage precursor material available for maturation. After the arrest of protein synthesis, maturation continues unabated until the limiting protei...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(66)90050-x
更新日期:1966-03-01 00:00:00
abstract::The genome of the single-stranded DNA phage f1 contains an intergenic region (IG), 508-nucleotides long, that does not code for any known protein. By use of a system of chimeric plasmids haboring different f1 fragments, we had previously shown that this region contains, in addition to the f1 'functional origin' of DNA...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90136-8
更新日期:1983-10-15 00:00:00