Abstract:
:Human immunodeficiency virus (HIV) encodes a transacting protein, Rev, which interacts with an RNA element (RRE) to mediate nuclear export of unspliced viral mRNA. Recently, the RNA constitutive transport elements (CTE) from Mason-Pfizer monkey virus (MPMV) and simian retrovirus type I (SRV-1) were shown to render Rev-independent expression of gag, pol, or env genes in subgenomic constructs of HIV-1 and to support replication of HIV-1 mutants lacking RRE and Rev. Since CTEs act in cis, in the absence of any viral regulatory proteins, it is widely believed that they interact directly with the cellular export machinery by means of RNA-protein interaction. In this report, Electrophoretic mobility shift and UV-crosslinking assays were carried out to identify nuclear proteins that interact specifically with CTEs from both MPMV and SRV-1. Two of the four proteins (65 and 40 kDa, respectively) that bound CTE RNA did not interact in the same assays with RNA of a nonfunctional CTE mutant generated by site-directed mutagenesis. Both proteins have been partially purified. The nature of these proteins and their roles in RNA intracellular trafficking are currently under investigation.
journal_name
Virologyjournal_title
Virologyauthors
Tang H,Xu Y,Wong-Staal Fdoi
10.1006/viro.1996.8407subject
Has Abstractpub_date
1997-02-17 00:00:00pages
333-9issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(96)98407-Xjournal_volume
228pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::There are more than 100 known adenovirus serotypes, including 50 human serotypes. They can infect all 5 major vertebrate classes but only Aviadenovirus infecting birds and Mastadenovirus infecting mammals have been well studied. CELO (chicken embryo lethal orphan) adenovirus is responsible for mild respiratory patholo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.05.011
更新日期:2008-08-15 00:00:00
abstract::Cells that express the human papillomavirus (HPV) type 16 E7 oncoprotein are predisposed to undergo apoptosis. Transgenic mice that have E7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. We established a cell cu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8851
更新日期:1997-12-08 00:00:00
abstract::Dengue viruses (DENV) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. DENV transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosqu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.049
更新日期:2007-02-20 00:00:00
abstract::Previously a new small subclass of SV40 large T antigen with a high-binding affinity to living target cells was characterized (J. Lange-Mutschler and R. Henning, 1983, Virology 127, 333-344.) In the present study the external binding process, particularly the tight linkage of T antigen to lipid of the target cells, wa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90176-4
更新日期:1984-07-30 00:00:00
abstract::The complete nucleotide sequence of the RSV cpts-248/404 live attenuated vaccine candidate was determined from cloned cDNA and was compared to that of the RSV A2/HEK7 wild-type, cold-passaged cp-RSV, and cpts-248 virus, which constitute the series of progenitor viruses. RSV cpts-248/404 is more attenuated and more tem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0618
更新日期:1996-11-15 00:00:00
abstract::Sequence heterogeneity of hepatitis B virus (HBV) is increasingly recognized to play a role in virus-host interaction. We have used a recently established method for HBV full-length genome amplification to search for novel types of HBV variants and to investigate further the sequence heterogeneity of HBV genome popula...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8898
更新日期:1997-12-22 00:00:00
abstract::The results of a previous study suggested that the poly(A) sequence in barley stripe mosaic virus (BSMV) RNA is intercalated between a 3'-terminal tyrosine-accepting structure and the 5'-terminal coding part of the BSMV genome. Here we show that poly(A)+ and poly(A)- fractions of BSMV RNA can be cleaved into two fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90064-2
更新日期:1982-05-01 00:00:00
abstract::The vaginal and rectal mucosae are the first line of cellular immune defense to sexually transmitted human immunodeficiency virus type 1 (HIV-1) entry. Thus, intraepithelial lymphocytes (IELs) may be important in the immune response to HIV infection. Here we investigated whether functional IELs in mucosal compartments...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.08.046
更新日期:2004-02-20 00:00:00
abstract::Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are local...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.12.012
更新日期:2014-02-01 00:00:00
abstract::Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90556-5
更新日期:1992-06-01 00:00:00
abstract::Influenza viruses of the H3N2 subtype similar to Aichi/2/68 and Victoria/3/75 persist in pigs many years after their antigenic counterparts have disappeared from humans (Shortridge et al. (1977). Science 19, 1454-1455). To provide information on the mechanism of conservation of these influenza viruses in pigs, the hem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90405-9
更新日期:1988-01-01 00:00:00
abstract::The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8608
更新日期:1997-06-23 00:00:00
abstract::Previously we have reported that the essential 3a movement gene of icosahedral cowpea chlorotic mottle virus (CCMV) can be functionally replaced by the 30-kDa movement gene of rod-shaped sunn-hemp mosaic virus (SHMV). Because plant RNA viruses differ in requiring or not requiring coat protein for systemic infection, w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8544
更新日期:1997-05-26 00:00:00
abstract::Influenza virus has a segmented genome composed of eight negative stranded RNA segments. Each segment is covered with NP forming ribonucleoproteins (vRNPs) and carries a copy of the heterotrimeric polymerase complex. As a rare phenomenon among the RNA viruses, the viral replication occurs in the nucleus and therefore ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.10.001
更新日期:2011-01-05 00:00:00
abstract::Poliovirus type 1 enters HeLa cells by receptor-mediated endocytosis as an intact virus. Up to 30 min after adsorption complete virus particles still containing VP4 and sedimenting with 156 S could be recovered from the cells. These virus particles were N-antigenic and infectious. Thirty minutes after adsorption the r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90286-9
更新日期:1989-02-01 00:00:00
abstract::Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90386-3
更新日期:1989-05-01 00:00:00
abstract::We have previously described a selective increase in HIV-DNA content in CCR5-negative lymphocytes from late stage HIV-infected patients. Here, we show that this increase occurred even in the absence of viral phenotypic switching from CCR5- to CXCR4-tropic. This leads us to hypothesize that early and late CCR5-tropic v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.003
更新日期:2006-04-10 00:00:00
abstract::Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-1 infection. In the pre...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.11.032
更新日期:2015-02-01 00:00:00
abstract::The mitotic spindle assembly checkpoint (SAC) ensures faithful chromosome segregation during mitosis by censoring kinetochore-microtubule interactions. It is frequently rendered dysfunctional during carcinogenesis causing chromosome missegregation and genomic instability. There are conflicting reports whether the HPV1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.06.006
更新日期:2012-10-10 00:00:00
abstract::The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9046
更新日期:1998-03-30 00:00:00
abstract::We have investigated a novel strategy for coexpressing two genes from a retroviral vector. The 5' nontranslated leader region of at least some picornavirus RNAs contains a sequence that can act as an internal ribosome entry site allowing initiation of translation at a downstream AUG codon in a 5' cap-independent manne...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90033-l
更新日期:1992-02-01 00:00:00
abstract::Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.022
更新日期:2018-02-01 00:00:00
abstract::Genetic variation in dengue 2 isolates from various geographic areas was examined by oligonucleotide fingerprinting of the 40 S genome RNA. Oligonucleotide maps of geographically isolated and epidemiologically unrelated viruses were very distinct. Direct comparison of the oligonucleotide map of the dengue 2 prototype ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90255-6
更新日期:1983-07-30 00:00:00
abstract::Female BALB/c mice were infected with coxsackievirus B3 in the diestrus, proestrus, estrus, or metestrus phases of the ovarian cycle. Cycle stage was determined by vaginal smear. All mice were killed 7 days after infection. Females infected in the diestrus and especially the proestrus phases developed myocarditis. CD4...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.06.051
更新日期:2004-12-05 00:00:00
abstract::The second smallest subgenomic messenger RNA, mRNA5, of the coronavirus infectious bronchitis virus includes in its "5' unique region" two separate open reading frames (5a and 5b), whose coding function has not so far been established, and thus it may represent a dicistronic messenger RNA. We report here that two poly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90094-6
更新日期:1992-01-01 00:00:00
abstract::A plasmid-based transfection system capable of generating infectious porcine circovirus type 2 (PCV2) was established. This system was then used in mutagenesis studies to investigate the involvement of a "conserved" nonanucleotide (which constitutes a portion of the loop sequence) located at the origin of DNA replicat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.03.037
更新日期:2004-06-20 00:00:00
abstract::Marburg virus (MBGV), for which no vaccines or treatments currently exist, causes an acute hemorrhagic fever with a high mortality rate in humans. We previously showed that immunization with either killed MBGV or a glycoprotein (GP) subunit prevented lethal infection in guinea pigs. In the studies reported here, an RN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9367
更新日期:1998-11-10 00:00:00
abstract::Prior studies with recombinant viruses constructed in vitro showed that the V2 domain of envelope gp120, in addition to the required V3 domain, enhances the efficiency of infection of primary macrophages by HIV-1. Present structural studies on the gp120s of these recombinant viruses using three human monoclonal antibo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1010
更新日期:1995-02-01 00:00:00
abstract::The elucidation of the antigenic structure of the S glycoprotein of murine coronaviruses will provide further understanding of the complex pathogenicity of these viruses. In order to identify linear antigenic determinants, the primary structure of the S glycoprotein of murine hepatitis virus strain A59 was analyzed wi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1376
更新日期:1994-08-01 00:00:00
abstract::Virions of southern bean mosaic virus (SBMV) show two distinct sensitivity patterns upon heating. Infectivity loss and capsid denaturation occur concurrently if virions are exposed at 50-55 degrees in 0.1 M sodium phosphate buffer, pH 7.5, or 0.1 M glycine-phosphate buffer, pH 9.0. Contrastingly, virions are inactivat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(81)90437-2
更新日期:1981-01-30 00:00:00