Abstract:
:Dengue viruses (DENV) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. DENV transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosquito with larval development in peridomestic water containers, and human reservoir hosts. All 4 serotypes of endemic DENV evolved independently from ancestral sylvatic viruses and have become both ecologically and evolutionarily distinct; this process may have involved adaptation to (i) peridomestic mosquito vectors and/or (ii) human reservoir hosts. To test the latter hypothesis, we assessed the ability of sylvatic and endemic DENV-2 strains, representing major genotypes from Southeast Asia, West Africa and the Americas, to replicate in two surrogate human model hosts: monocyte-derived, human dendritic cells (moDCs), and mice engrafted with human hepatoma cells. Although the various DENV-2 strains showed significant inter-strain variation in mean replication titers in both models, no overall difference between sylvatic and endemic strains was detected in either model. Our findings suggest that emergence of endemic DENV strains from ancestral sylvatic strains may not have required adaptation to replicate more efficiently in human reservoir hosts, implying that the potential for re-emergence of sylvatic dengue strains into the endemic cycle is high. The shared replication profiles of the American endemic and sylvatic strains suggest that American strains have maintained or regained the ancestral phenotype.
journal_name
Virologyjournal_title
Virologyauthors
Vasilakis N,Shell EJ,Fokam EB,Mason PW,Hanley KA,Estes DM,Weaver SCdoi
10.1016/j.virol.2006.08.049subject
Has Abstractpub_date
2007-02-20 00:00:00pages
402-12issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00622-2journal_volume
358pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The coat protein (CP) of alfalfa mosaic virus (AMV) strain 425 assembles to bacilliform or rod-shaped particles in the presence of nucleic acids or to T = 1 empty icosahedral particles in the absence of nucleic acids. To study the determinants of CP assembly, recombinant CPs (rCPs) that contained a (His)(6) region wer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9805
更新日期:1999-07-20 00:00:00
abstract::Hepatitis C virus (HCV) core protein constitutes a viral nucleocapsid and may possess multiple functions. In this study, we demonstrated the homotypic interaction and multimerization of HCV core protein in vitro and in vivo. By using a yeast two-hybrid system, we showed that the amino-terminal hydrophilic portion (ami...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0164
更新日期:1996-04-01 00:00:00
abstract::The dependence of retroviral replication on cell proliferation was described as early as 1958, although different classes of retroviruses are able to infect non-dividing cells with different efficiencies. For example, the human immunodeficiency virus (HIV) and other lentiviruses infect most non-dividing cells nearly a...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2005.09.012
更新日期:2006-01-05 00:00:00
abstract::Previous studies with monoclonal antibodies of the antigenic structure of bovine leukemia virus (BLV) envelope glycoprotein (gp51) have identified three epitopes (F, G, H) directly involved in the infectivity of BLV, F, G, and H lost their reactivity with the respective monoclonal antibodies after treatment with a red...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90037-8
更新日期:1989-03-01 00:00:00
abstract::The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0368
更新日期:1996-07-01 00:00:00
abstract::The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1121
更新日期:1993-03-01 00:00:00
abstract::Recently, we reported that in vitro and in vivo persistent infection of neurons by lymphocytic choriomeningitis virus (LCMV) downregulated GAP43 expression, a protein involved in neuronal plasticity associated with learning and memory. Here, we investigated the transcriptional and posttranscriptional events involved. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8458
更新日期:1997-04-14 00:00:00
abstract::The preS(21-47) sequence of the hepatitis B virus (HBV) envelope protein is involved in binding of the virus to cell receptors. A protein similarity search revealed a partial homology between this sequence and a segment of the human immunoglobulin A (IgA) heavy chain constant region, suggesting that the cell attachmen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90368-2
更新日期:1990-10-01 00:00:00
abstract::Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.003
更新日期:2008-07-05 00:00:00
abstract::The most recent introduction of an avian influenza A virus without reassortment into mammals occurred in 1979 when H1N1 strains could be isolated from diseased pigs in northern Europe. This newly introduced avian virus formed a stable lineage in pigs and, in the meantime, spread all over Europe. In 1991 highly pathoge...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1344
更新日期:1994-07-01 00:00:00
abstract::In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90525-t
更新日期:1992-06-01 00:00:00
abstract::An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope located within 58 amin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90457-m
更新日期:1991-10-01 00:00:00
abstract::The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of int...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90494-c
更新日期:1990-07-01 00:00:00
abstract::Tobacco mosaic virus (TMV) is widespread in China and causes considerable economic losses to tobacco production. The molecular epidemiology of this virus is, however, poorly understood. In this study, we sequenced the genomes of 51 TMV isolates from five tobacco-producing regions in China and investigated the dispersa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.12.001
更新日期:2019-02-01 00:00:00
abstract::Lymphocytic choriomeningitis virus (LCMV) Armstrong caused disordered growth and glucose metabolism secondary to growth hormone deficiency in infected C3H/St mice. In contrast, LCMV strain WE replicated in fewer growth hormone-producing cells, failed to disrupt growth hormone synthesis, and did not cause growth hormon...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90431-3
更新日期:1985-04-15 00:00:00
abstract::Hepatitis B virus (HBV) clone 4B replicated much more efficiently than clone 2A of the same genotype. Introduction of its T1753C, A1762T, G1764A, and C1766T core promoter mutations into the 2A genome greatly enhanced genome replication and suppressed HBeAg expression. Here we show that these effects are mediated by tr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.03.002
更新日期:2009-05-10 00:00:00
abstract::The gene encoding the M1 protein of sonchus yellow net virus (SYNV), a plant rhabdovirus, has been sequenced and identified by Western blot analysis of SYNV proteins using antibodies directed against a fusion protein derived from a portion of the sequenced gene. The M1 gene is positioned between nucleotides 4039 and 5...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90289-4
更新日期:1990-11-01 00:00:00
abstract::Early region 1 (E1) of the human adenoviruses has many intriguing properties which have prompted numerous mutational studies to help delineate and characterize the domains responsible for these functions. In mutational analyses being done currently, the E1 region is usually cloned into a bacterial plasmid where it is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90302-9
更新日期:1988-04-01 00:00:00
abstract::Cauliflower mosaic virus gene VI product (P6) is an essential protein that forms cytoplasmic, inclusion bodies (IBs). P6 contains four regions involved in self-association, termed D1-D4. D3 binds to D1, along with D4 and contains a spacer region (termed D3b) between two RNA-binding domains. Here we show D3b binds full...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.11.017
更新日期:2015-02-01 00:00:00
abstract::At high concentrations, several monoclonal antibodies to the G1 glycoprotein of La Crosse (LAC) virus aggregated the virus. To determine whether this accounted for the neutralization, the monoclonal antibodies were digested to make Fab fragments. With one exception, each monovalent antibody neutralized LAC virus to th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90073-k
更新日期:1991-02-01 00:00:00
abstract::Because phosphorylation of the infectious bronchitis virus (IBV) nucleocapsid protein (N) may regulate its multiple roles in viral replication, the dynamics of N phosphorylation were examined. 32P-orthophosphate labeling and Western blot analyses confirmed that N was the only viral protein that was phosphorylated. Pul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.04.029
更新日期:2005-08-15 00:00:00
abstract::Nucleotide sequencing studies (D. J. McGeoch, A. Dolan, S. Donald, and F. Rixon, 1985, J. Mol. Biol. 181, 1-14) have indicated that herpes simplex virus type 1 (HSV-1) has a coding sequence, referred to as US7, between the genes for the glycoproteins D and E (gD and gE). Northern blot analysis and nucleotide sequencin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90002-4
更新日期:1986-08-01 00:00:00
abstract::Equine infectious anemia virus (EIAV) is responsible of acute disease episodes characterized by fever, anemia, thrombocytopenia and anorexia in equids. The high mutation rate in EIAV genome limited the number of full genome sequences availability. In the present study, we used the SureSelect target enrichment system w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.08.016
更新日期:2019-11-01 00:00:00
abstract::We have studied the assembly of human immunodeficiency virus (HIV-1) Gag-B-galactosidase (Gag-B-gal; GBG) fusion proteins into HIV particles in the presence of HIV Gag proteins. Release of fusion proteins from cells was measured by assay of media versus cellular B-gal activities and was dependent on co-expression of u...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1215
更新日期:1994-05-01 00:00:00
abstract::Infection with bovine papillomavirus type 1 (BPV1) or BPV2 induces fibropapillomas in cows and skin sarcoids in horses. Prophylactic vaccination targeting BPV1 and BPV2 may reduce the incidence of these economically important diseases. The L1 major capsid proteins of BPV1 and BPV2 were expressed in Sf-9 insect cells a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.036
更新日期:2009-10-10 00:00:00
abstract::Human adenovirus type 5 (Ad5) has been the most popular platform for the development of oncolytic Ads. Alternative Ad serotypes with low seroprevalence might allow for improved anticancer efficacy in Ad5-immune patients. We studied the safety and efficacy of rare serotypes Ad6, Ad11 and Ad35. In vitro cytotoxicity of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.038
更新日期:2009-11-25 00:00:00
abstract::A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35S promoter in an agroinfection vector. The biological activity o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.9945
更新日期:1995-12-01 00:00:00
abstract::Turnip leaves infected with turnip crinkle virus accumulate a 35-kd polypeptide which comigrates with the major protein from isolated virions. RNA from TCV virions directs the synthesis of a number of polypeptides in vitro including a 35-kd protein which is immunoprecipitable with antiserum to virus particles. Transla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90326-9
更新日期:1982-04-15 00:00:00
abstract::About 100 nucleotides of DNA sequence at the 5' noncoding region of the Choristoneura biennis entomopoxvirus spheroidin gene was chemically synthesized and inserted into a vaccinia expression vector, interrupting the vaccinia thymidine kinase gene. When the bacterial beta-galactosidase gene was introduced downstream o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90070-r
更新日期:1991-02-01 00:00:00
abstract::Hepatitis C virus (HCV) encodes a polyprotein that is processed to produce the structural and nonstructural proteins of the virus. Nonstructural protein 3 (NS3) is a serine proteinase that cleaves the polyprotein to release the NS4A, NS4B, NS5A, and NS5B proteins. To characterize the substrate specificity of NS3, we s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1520
更新日期:1994-10-01 00:00:00