Abstract:
:A plasmid-based transfection system capable of generating infectious porcine circovirus type 2 (PCV2) was established. This system was then used in mutagenesis studies to investigate the involvement of a "conserved" nonanucleotide (which constitutes a portion of the loop sequence) located at the origin of DNA replication of PCV2 with respect to viral protein synthesis, DNA self-replication, and progeny virus production. The results demonstrated that an octanucleotide (AGTATTAC) embedded in the loop sequence is essential for virus replication. This octanucleotide can be further condensed to an essential core element (ECE) represented by AxTAxTAC. The positions specified by the indicated nucleotides are critical for viral DNA replication and stable infectious virus production, and they cannot be substituted by other bases, while the positions indicated by x can accept variable bases and yield stable progeny viruses.
journal_name
Virologyjournal_title
Virologyauthors
Cheung AKdoi
10.1016/j.virol.2004.03.037subject
Has Abstractpub_date
2004-06-20 00:00:00pages
28-36issue
1eissn
0042-6822issn
1096-0341pii
S0042682204001862journal_volume
324pub_type
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