Superinfection exclusion by Citrus tristeza virus does not correlate with the production of viral small RNAs.


:Superinfection exclusion (SIE), a phenomenon in which a preexisting viral infection prevents a secondary infection with the same or closely related virus, has been described for different viruses, including important pathogens of humans, animals, and plants. Several mechanisms acting at various stages of the viral life cycle have been proposed to explain SIE. Most cases of SIE in plant virus systems were attributed to induction of RNA silencing, a host defense mechanism that is mediated by small RNAs. Here we show that SIE by Citrus tristeza virus (CTV) does not correlate with the production of viral small interfering RNAs (siRNAs). CTV variants, which differed in the SIE ability, had similar siRNAs profiles. Along with our previous observations that the exclusion phenomenon requires a specific viral protein, p33, the new data suggest that SIE by CTV is highly complex and appears to use different mechanisms than those proposed for other viruses.






Folimonova SY,Harper SJ,Leonard MT,Triplett EW,Shilts T




Has Abstract


2014-11-01 00:00:00














  • Antigenic and structural properties of a paramyxovirus simian virus 41 (SV41) reveal a close relationship with human parainfluenza type 2 virus.

    abstract::Seven structural component proteins of a paramyxovirus simian virus 41 (SV41) were identified with the aid of monoclonal antibodies prepared against SV41 and human parainfluenza type 2 virus (PIV2). The nucleoprotein is antigenically very close to that of PIV2, while it is comparatively far from that of simian virus 5...


    pub_type: 杂志文章


    authors: Tsurudome M,Bando H,Nishio M,Iwamoto Y,Kawano M,Kondo K,Komada H,Ito Y

    更新日期:1990-12-01 00:00:00

  • Four-gene-combination DNA vaccine protects mice against a lethal vaccinia virus challenge and elicits appropriate antibody responses in nonhuman primates.

    abstract::Two major infectious forms of vaccinia virus (VACV) have been described: the intracellular mature virion (IMV), and the extracellular enveloped virion (EEV). Due to their stability in the environment, IMVs play a predominant role in host-to-host transmission, whereas EEVs play an important role in dissemination within...


    pub_type: 杂志文章


    authors: Hooper JW,Custer DM,Thompson E

    更新日期:2003-02-01 00:00:00

  • Ceramide formation mediated by acid sphingomyelinase facilitates endosomal escape of caliciviruses.

    abstract::Our recent results demonstrated that bile acids facilitate virus escape from the endosomes into the cytoplasm for successful replication of porcine enteric calicivirus (PEC). We report a novel finding that bile acids can be substituted by cold treatment for endosomal escape and virus replication. This endosomal escape...


    pub_type: 杂志文章


    authors: Shivanna V,Kim Y,Chang KO

    更新日期:2015-09-01 00:00:00

  • In vitro excision and replication of 5' telomeres of minute virus of mice DNA from cloned palindromic concatemer junctions.

    abstract::HeLa cell extracts containing wild-type copies of the minute virus of mice NS-1 polypeptide produced from a recombinant vaccinia virus vector could support the excision and replication of viral 5' telomeres from cloned concatemer junction fragments. Resolution did not occur if wild-type NS-1 was omitted from the extra...


    pub_type: 杂志文章


    authors: Cotmore SF,Nuesch JP,Tattersall P

    更新日期:1992-09-01 00:00:00

  • Cellular bioenergetics, caspase activity and glutathione in murine lungs infected with influenza A virus.

    abstract::Inhibition of cellular respiration, oxidation of glutathione and induction of apoptosis have been reported in epithelial cells infected in vitro with influenza A virus (IAV). Here, the same biomarkers were investigated in vivo by assessing the lungs of BALB/c mice infected with IAV. Cellular respiration declined on da...


    pub_type: 杂志文章


    authors: Alsuwaidi AR,Almarzooqi S,Albawardi A,Benedict S,Kochiyil J,Mustafa F,Hartwig SM,Varga SM,Souid AK

    更新日期:2013-11-01 00:00:00

  • The ecology of primate retroviruses - an assessment of 12 years of retroviral studies in the Taï national park area, Côte d׳Ivoire.

    abstract::The existence and genetic make-up of most primate retroviruses was revealed by studies of bushmeat and fecal samples from unhabituated primate communities. For these, detailed data on intra- and within-species contact rates are generally missing, which makes identification of factors influencing transmission a challen...


    pub_type: 杂志文章


    authors: Gogarten JF,Akoua-Koffi C,Calvignac-Spencer S,Leendertz SA,Weiss S,Couacy-Hymann E,Koné I,Peeters M,Wittig RM,Boesch C,Hahn BH,Leendertz FH

    更新日期:2014-07-01 00:00:00

  • Simvastatin and oseltamivir combination therapy does not improve the effectiveness of oseltamivir alone following highly pathogenic avian H5N1 influenza virus infection in mice.

    abstract::Nonspecific anti-inflammatory drugs have been purported to reduce the burden of severe influenza disease. We demonstrate that, unlike oseltamivir administration, simvastatin administration did not reduce morbidity, mortality, or viral load of mice infected with H1N1 or H5N1 viruses. No added benefit to the efficacy of...


    pub_type: 杂志文章


    authors: Belser JA,Szretter KJ,Katz JM,Tumpey TM

    更新日期:2013-04-25 00:00:00

  • Functional simian immunodeficiency virus Gag-specific CD8+ intraepithelial lymphocytes in the mucosae of SIVmac251- or simian-human immunodeficiency virus KU2-infected macaques.

    abstract::The vaginal and rectal mucosae are the first line of cellular immune defense to sexually transmitted human immunodeficiency virus type 1 (HIV-1) entry. Thus, intraepithelial lymphocytes (IELs) may be important in the immune response to HIV infection. Here we investigated whether functional IELs in mucosal compartments...


    pub_type: 杂志文章


    authors: Stevceva L,Moniuszko M,Alvarez X,Lackner AA,Franchini G

    更新日期:2004-02-20 00:00:00

  • Vaccination with herpes simplex virus type 1 glycoprotein K impairs clearance of virus from the trigeminal ganglia resulting in chronic infection.

    abstract::Following primary ocular infection, HSV-1 establishes a latent infection in the trigeminal ganglia with the complete absence of detectable infectious virus. Recently, we showed that vaccination of BALB/cJ with HSV-1 glycoprotein K (gK), but not other HSV-1 glycoproteins, significantly exacerbated the severity of HSV-1...


    pub_type: 杂志文章


    authors: Ghiasi H,Cai S,Nesburn AB,Wechsler SL

    更新日期:1996-10-01 00:00:00

  • Viral cytopathogenicity correlated with integration of ubiquitin-coding sequences.

    abstract::The RNA genomes of cytopathogenic bovine viral diarrhea virus (BVDV) isolates contain insertions highly homologous to cellular sequences. For two of them the insert was identified as ubiquitin coding sequence. The genome of BVDV Osloss contains exactly one ubiquitin gene monomer. In the case of BVDV CP1 the cellular i...


    pub_type: 杂志文章


    authors: Meyers G,Tautz N,Dubovi EJ,Thiel HJ

    更新日期:1991-02-01 00:00:00

  • Immune protection conferred by the baculovirus-related glycoprotein of Thogoto virus (Orthomyxoviridae).

    abstract::The coding region of segment 4 of Thogoto (THO) virus, a tick-borne member of the Orthomyxoviridae, was expressed in a baculovirus system under the control of the polyhedrin promoter. This construct expressed authentic envelope glycoprotein as determined by size and antigenic reactivity with a panel of monoclonal anti...


    pub_type: 杂志文章


    authors: Jones LD,Morse MA,Marriott AC,Nuttall PA

    更新日期:1995-10-20 00:00:00

  • Establishment of cell lines persistently infected with foot-and-mouth disease virus.

    abstract::Cell lines persistently infected with foot-and-mouth disease virus (FMDV) have been established by growth of BHK-21 (c-13) or IBRS-2 (c-26) that survived standard cytolytic infections with FMDV. They maintain cytoplasmic FMDV RNA sequences, as shown by dot blot hybridization tests, using cloned FMDV cDNA as probes. Ce...


    pub_type: 杂志文章


    authors: de la Torre JC,Dávila M,Sobrino F,Ortín J,Domingo E

    更新日期:1985-08-01 00:00:00

  • The large subunit of herpes simplex virus type 1 ribonucleotide reductase: expression in Escherichia coli and purification.

    abstract::The open reading frame of the large subunit (R1) of herpes simplex virus type 1 (HSV-1) ribonucleotide reductase has been positioned downstream of the phage T7 gene 10 promoter in the expression vector, pET. Transformation of this recombinant plasmid into Escherichia coli BL21 DE3 cells containing the T7 RNA polymeras...


    pub_type: 杂志文章


    authors: Furlong J,Conner J,McLauchlan J,Lankinen H,Galt C,Marsden HS,Clements JB

    更新日期:1991-06-01 00:00:00

  • Analysis of human immunodeficiency virus type 1 promoter insertion in vivo.

    abstract::Our study reports the occurrence of HIV-1 insertional activation events in vivo. Using a previously described PCR assay, small aliquots of broncho-alveolar lavage (BAL) cells obtained from AIDS patients were analyzed. Nine percent (5/54) of the aliquots contained proviral-host sequence transcripts indicating HIV-1 pro...


    pub_type: 杂志文章


    authors: Raineri I,Solèr M,Senn HP

    更新日期:1995-04-01 00:00:00

  • Human papillomavirus type 16 E7 protein expressed in Escherichia coli and monkey COS-1 cells: immunofluorescence detection of the nuclear E7 protein.

    abstract::Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...


    pub_type: 杂志文章


    authors: Sato H,Watanabe S,Furuno A,Yoshiike K

    更新日期:1989-05-01 00:00:00

  • TLR3-dependent upregulation of RIG-I leads to enhanced cytokine production from cells infected with the parainfluenza virus SV5.

    abstract::Here we address the role of RIG-I and TLR3 in differential cytokine responses against Simian Virus 5 (SV5) and two distinct cytokine inducing SV5 mutants. IFN-beta and IL-6 secretion was induced by infection with P/V-CPI-, an SV5 mutant with P/V substitutions, and were reduced by either siRNA-mediated knockdown of RIG...


    pub_type: 杂志文章


    authors: Manuse MJ,Parks GD

    更新日期:2010-02-05 00:00:00

  • Epstein-Barr virus BRLF1 inhibits transcription of IRF3 and IRF7 and suppresses induction of interferon-beta.

    abstract::Activation of interferon regulatory factors (IRFs) 3 and 7 is essential for the induction of Type I interferons (IFN) and innate antiviral responses, and herpesviruses have evolved mechanisms to evade such responses. We previously reported that Epstein-Barr virus BZLF1, an immediate-early (IE) protein, inhibits the fu...


    pub_type: 杂志文章


    authors: Bentz GL,Liu R,Hahn AM,Shackelford J,Pagano JS

    更新日期:2010-06-20 00:00:00

  • Foot-and-mouth disease virus (FMDV) with a stable FLAG epitope in the VP1 G-H loop as a new tool for studying FMDV pathogenesis.

    abstract::Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and g...


    pub_type: 杂志文章


    authors: Lawrence P,Pacheco JM,Uddowla S,Hollister J,Kotecha A,Fry E,Rieder E

    更新日期:2013-02-05 00:00:00

  • Nucleotide sequences within the U5 region of the viral RNA genome are the major determinants for an human immunodeficiency virus type 1 to maintain a primer binding site complementary to tRNA(His).

    abstract::The initiation of reverse transcription of the human immunodeficiency virus type 1 (HIV-1) genome requires cellular tRNA(Lys,3) as a primer and occurs at a site in the viral RNA genome, designated as the primer binding site (PBS), which is complementary to the 3'-terminal 18 nucleotides of tRNA(Lys,3). We previously d...


    pub_type: 杂志文章


    authors: Zhang Z,Kang SM,LeBlanc A,Hajduk SL,Morrow CD

    更新日期:1996-12-15 00:00:00

  • Potential of ancestral sylvatic dengue-2 viruses to re-emerge.

    abstract::Dengue viruses (DENV) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. DENV transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosqu...


    pub_type: 杂志文章


    authors: Vasilakis N,Shell EJ,Fokam EB,Mason PW,Hanley KA,Estes DM,Weaver SC

    更新日期:2007-02-20 00:00:00

  • Quantification of infectious HIV-1 plasma viral load using a boosted in vitro infection protocol.

    abstract::Methods currently used for HIV-1 viral load measurements are very sensitive, but cannot distinguish between infectious and noninfectious particles. Here we describe the development of a novel, sensitive, and highly reproducible method that allows rapid isolation and quantification of infectious particles from patient ...


    pub_type: 杂志文章


    authors: Rusert P,Fischer M,Joos B,Leemann C,Kuster H,Flepp M,Bonhoeffer S,Günthard HF,Trkola A

    更新日期:2004-08-15 00:00:00

  • Alternative forms of a strain-specific neutralizing antigenic site on the Sindbis virus E2 glycoprotein.

    abstract::Experiments with monoclonal antibodies raised against two laboratory strains of Sindbis virus, SB and SIN, suggested the existence of a strain-specific neutralizing antigenic site (E2-b) on the E2 glycoprotein. A comparison of monoclonal antibody binding patterns and E2 glycoprotein gene sequences of six laboratory st...


    pub_type: 杂志文章


    authors: Davis NL,Pence DF,Meyer WJ,Schmaljohn AL,Johnston RE

    更新日期:1987-11-01 00:00:00

  • The effect of ribavirin and IMPDH inhibitors on hepatitis C virus subgenomic replicon RNA.

    abstract::The recent development of in vitro hepatitis C virus (HCV) RNA replication systems has provided useful tools for studying the intracellular anti-HCV activity of ribavirin. Ribavirin has been shown to: (1) induce "error catastrophe" in poliovirus, Proc. Natl. Acad. Sci. USA 98, 6895-6900), (2) be a pseudo-substrate of ...


    pub_type: 杂志文章


    authors: Zhou S,Liu R,Baroudy BM,Malcolm BA,Reyes GR

    更新日期:2003-06-05 00:00:00

  • HPV-18 immortalization of human keratinocytes.

    abstract::The oncogenic potential of human papillomavirus type 18 which is found in a significant number of cervical and penile cancer biopsies was tested in primary human keratinocytes derived from neonatal foreskin. Viral DNA and a gene for resistance to neomycin were introduced into these cells by calcium phosphate transfect...


    pub_type: 杂志文章


    authors: Kaur P,McDougall JK

    更新日期:1989-11-01 00:00:00

  • Vaccinia virus gene D12L encodes the small subunit of the viral mRNA capping enzyme.

    abstract::Vaccinia virus gene D12L, which lies between nucleotides 14,350 and 13,487 in the HindIII D fragment, is transcribed at early times in infection and is capable of encoding a protein 287 amino acids in length with a predicted molecular mass of 33,331. A polyclonal antiserum was raised in rabbits to a fusion protein con...


    pub_type: 杂志文章


    authors: Niles EG,Lee-Chen GJ,Shuman S,Moss B,Broyles SS

    更新日期:1989-10-01 00:00:00

  • Influenza virus genome C4 promoter/origin attenuates its transcription and replication activity by the low polymerase recognition activity.

    abstract::A natural variation is observed at position 4 of the 3'-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus...


    pub_type: 杂志文章


    authors: Jiang H,Zhang S,Wang Q,Wang J,Geng L,Toyoda T

    更新日期:2010-12-20 00:00:00

  • Sequence variability and function of measles virus 3' and 5' ends and intercistronic regions.

    abstract::Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3' and 5' ends of the MV genome were identified and sequenced, and the...


    pub_type: 杂志文章


    authors: Crowley JC,Dowling PC,Menonna J,Silverman JI,Schuback D,Cook SD,Blumberg BM

    更新日期:1988-06-01 00:00:00

  • ERV3, a full-length human endogenous provirus: chromosomal localization and evolutionary relationships.

    abstract::A full-length human endogenous provirus termed ERV3 was isolated from a human fetal recombinant DNA library by low stringency hybridization with two probes: baboon endogenous virus LTR; and a pol-env subclone from the endogenous chimpanzee provirus, CH2. DNA sequencing within the clone and comparisons with other retro...


    pub_type: 杂志文章


    authors: O'Connell C,O'Brien S,Nash WG,Cohen M

    更新日期:1984-10-30 00:00:00

  • Lysosomal localization and mechanism of membrane penetration influence nonenveloped virus activation of the NLRP3 inflammasome.

    abstract::Adenovirus (Ad) endosomal membrane penetration activates the NLRP3 inflammasome by releasing lysosomal cathepsin B (catB) into the cytoplasm. We therefore examined the extent to which inflammasome activation correlates with Ad colocalization with catB-enriched lysosomes. Inflammasome activation, is greater during infe...


    pub_type: 杂志文章


    authors: Barlan AU,Danthi P,Wiethoff CM

    更新日期:2011-04-10 00:00:00

  • NS-1 and NS-2 proteins may act synergistically in the cytopathogenicity of parvovirus MVMp.

    abstract::The interaction of parvovirus minute virus of mice (prototype strain, MVMp) with simian virus 40 (SV40)-transformed human cells (NB-E) was investigated by means of transfection with MVMp molecular clones derived from the infectious recombinant plasmid (pMM984). pMM984 inhibits stable transformation of NB-E cells to ge...


    pub_type: 杂志文章


    authors: Brandenburger A,Legendre D,Avalosse B,Rommelaere J

    更新日期:1990-02-01 00:00:00