Abstract:
:The minimal 5' initiation of assembly sequence for papaya mosaic virus (PMV) has been determined by the generation of successive 3' deletion clones from a full-length cDNA copy of the PMV genome followed by in vitro assembly of RNA transcripts. Sequences closest to the 5' terminus were cloned into plasmid vectors with synthetic complementary oligonucleotides representing viral sequences. The in vitro initiation sequence has been narrowed down to 38-47 nucleotides at the 5' terminus which are capable of initiation alone or in conjunction with homologous or heterologous sequences. The minimum initiation sequence is rich in adenosine and cytidine and very poor in uridine nucleotides and lacks any discernible secondary structure.
journal_name
Virologyjournal_title
Virologyauthors
Sit TL,Leclerc D,AbouHaidar MGdoi
10.1006/viro.1994.1118subject
Has Abstractpub_date
1994-02-15 00:00:00pages
238-42issue
1eissn
0042-6822issn
1096-0341pii
S0042682284711184journal_volume
199pub_type
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