Abstract:
:Human norovirus is a dominant cause of acute gastroenteritis around the world. Several norovirus disinfectants label citric acid as an active ingredient. In this study, we showed that norovirus virus-like particles (VLPs) treated with citrate buffer caused the particles to alter their morphology, including increased diameters associated with a new ring-like structure. We also found that epitopes on the protruding (P) domain on these particles were more readily accessible to antibodies after the citrate treatment. These results suggested that citrate had a direct effect on the norovirus particles. Using X-ray crystallography, we showed that the P domain bound citrate from lemon juice and a disinfectant containing citric acid. Importantly, citrate binds at the histo-blood group antigen binding pocket, which are attachment factors for norovirus infections. Taken together, these new findings suggested that it might be possible to treat/reduce norovirus infections with citrate, although further studies are needed.
journal_name
Virologyjournal_title
Virologyauthors
Koromyslova AD,White PA,Hansman GSdoi
10.1016/j.virol.2015.07.009subject
Has Abstractpub_date
2015-11-01 00:00:00pages
199-204eissn
0042-6822issn
1096-0341pii
S0042-6822(15)00328-1journal_volume
485pub_type
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