Abstract:
:Gene transfer approaches using viruses such human adenovirus (HAdV) may provide an alternative treatment for diseases involving hematopoietic cells. Better understanding of the cellular mechanisms by which the HAdV introduces DNA into these cells should help in vector design. We examined HAdV intracellular delivery in several cell lines including B and T lymphocytes. We demonstrated that HAdV resistance in most B lymphocytes is the result of moderate HAdV uptake. In contrast, high levels of coxsackie and HAdV receptor (hCAR) are expressed on the surface of HSB2 (T cells), allowing efficient binding and uptake but no transgene expression, probably because of deficient endosomolysis and subsequent exocytose. This work demonstrates the existence of hCAR-dependent and -independent endocytic route in hematopoietic cells. Moreover, it precises the intracellular barriers to be overcome by HAdV in such cells to be infectious and gives previous information's to design new vectors for gene transfer.
journal_name
Virologyjournal_title
Virologyauthors
Colin M,Renaut L,Mailly L,D'Halluin JCdoi
10.1016/j.virol.2003.09.043subject
Has Abstractpub_date
2004-03-01 00:00:00pages
23-39issue
1eissn
0042-6822issn
1096-0341pii
S0042682203007438journal_volume
320pub_type
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