Abstract:
:Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated entry of retroviral-, rhabdoviral and filoviral particles depends on CatB/CatL activity and that this effect is cell line-independent. Moreover, we show that the human cell line Calu-3, which expresses low amounts of CatL, is largely resistant to entry driven by diverse filovirus GPs. Finally, we demonstrate that Calu-3 cell entry mediated by certain filovirus GPs can be rescued upon directed expression of CatL or DC-SIGN. Our results identify Calu-3 cells as largely resistant to filovirus GP-driven entry and demonstrate that entry is limited at the stage of virion attachment and GP priming.
journal_name
Virologyjournal_title
Virologyauthors
González-Hernández M,Müller A,Hoenen T,Hoffmann M,Pöhlmann Sdoi
10.1016/j.virol.2019.03.020subject
Has Abstractpub_date
2019-06-01 00:00:00pages
22-29eissn
0042-6822issn
1096-0341pii
S0042-6822(19)30091-1journal_volume
532pub_type
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