Abstract:
:To identify new genes affecting Tomato bushy stunt virus (TBSV) replication in yeast model host, we are studying protein families, whose members have been identified during previous high throughput screening. In this paper, we have characterized the WW domain-containing protein family from yeast and plants. We find that, in addition to Rsp5 E3 ubiquitin ligase, yeast Wwm1 and Prp40 and three Arabidopsis WW domain-containing proteins are strong inhibitors of TBSV replication. The tombusvirus replicase complex isolated from yeast with down-regulated Wwm1 protein level was more active. Accumulation of viral p92(pol) was reduced when Wwm1 was over-expressed, suggesting that the stability of p92(pol) might be reduced, as observed with Rsp5. Moreover, replication of two insect RNA viruses is also inhibited by Wwm1 and Rsp5, suggesting that WW domain-containing proteins might have broad regulatory effects on RNA viruses. Thus, artificial antiviral proteins with WW domains could be useful antiviral strategy.
journal_name
Virologyjournal_title
Virologyauthors
Qin J,Barajas D,Nagy PDdoi
10.1016/j.virol.2012.01.020subject
Has Abstractpub_date
2012-05-10 00:00:00pages
106-19issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(12)00057-8journal_volume
426pub_type
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