Zika virus NS5 nuclear accumulation is protective of protein degradation and is required for viral RNA replication.

Abstract:

:Zika virus (ZIKV) nonstructural protein 5 (NS5) is a multifunctional protein possessing methyltransferase and RNA-dependent RNA polymerase activities. In the present study, we have carried out an extensive mutagenesis analysis to determine the importance of nuclear localization sequences (NLS) of NS5 in its nuclear accumulation and ZIKV replication. Deletion mutagenesis analysis demonstrated that the bipartite NLS consisting of importin β1 (βNLS) and importin α/β-recognized NLS (α/βNLS) is required for NS5 nuclear accumulation. Deletion of βNLS, α/βNLS, or both as well as R393A and R393N mutations severely impaired NS5 nuclear import and consequently conferred NS5 degradation. The R393A and R393N mutations also ablated viral RNA replication and virus production. Treatment of ZIKV-infected cells with importin α/β-NS5 interaction inhibitors ivermectin or 4-HPR resulted in a rapid degradation of NS5 similar to the R393 A/N mutations. Collectively, these findings suggest that NS5 nuclear accumulation protects NS5 from cytoplasmic degradation and therefore is required for viral RNA replication.

journal_name

Virology

journal_title

Virology

authors

Ji W,Luo G

doi

10.1016/j.virol.2019.10.010

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

124-135

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(19)30300-9

journal_volume

541

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Anti-gD monoclonal antibodies inhibit cell fusion induced by herpes simplex virus type 1.

    abstract::Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(83)90409-9

    authors: Noble AG,Lee GT,Sprague R,Parish ML,Spear PG

    更新日期:1983-08-01 00:00:00

  • Mouse xenotropic viruses can inhibit cell transformation.

    abstract::Mammalian type C retroviruses can be detected by their induction of foci of cell transformation in S+I - cells. We have noted that certain subtypes of the mouse xenotropic type C retrovirus (MuLV) inhibit this cell alteration. This inhibition, associated with intact virions, is irreversible and gives the infected cell...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(87)90044-4

    authors: Hoffman AD,Levy JA

    更新日期:1987-09-01 00:00:00

  • MMTV does not encode viral microRNAs but alters the levels of cancer-associated host microRNAs.

    abstract::Mouse mammary tumor virus (MMTV) induces breast cancer in mice in the absence of known virally-encoded oncogenes. Tumorigenesis by MMTV is thought to occur primarily through insertional mutagenesis, leading to the activation of cellular proto-oncogenes and outgrowth of selected cells. Here we investigated whether MMTV...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2017.09.030

    authors: Kincaid RP,Panicker NG,Lozano MM,Sullivan CS,Dudley JP,Mustafa F

    更新日期:2018-01-01 00:00:00

  • Transforming potential of deletion mutants of the SV40 T antigen coding gene in Syrian hamster cells.

    abstract::Thymidine kinase-deficient syrian hamster cells were cotransfected with recombinant plasmids containing the thymidine kinase (TK) gene of Herpes Simplex Virus Type 1, and either intact or partially deleted SV40 T antigen-coding genes. The transformants were selected by their ability to grow in gHAT medium. After selec...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(84)90287-3

    authors: Hirschhorn RR,Mercer WE,Liu HT,Baserga R

    更新日期:1984-04-15 00:00:00

  • Mason-Pfizer monkey virus Gag proteins interact with the human sumo conjugating enzyme, hUbc9.

    abstract::Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/s0042-6822(03)00348-9

    authors: Weldon RA Jr,Sarkar P,Brown SM,Weldon SK

    更新日期:2003-09-15 00:00:00

  • Nucleotide sequence analysis of the S genomic segment of Prospect Hill virus: comparison with the prototype Hantavirus.

    abstract::The genomic S RNA segment of Prospect Hill virus (PHV), a member of the Hantavirus genus, was molecularly cloned and the nucleotide sequence of the cDNA determined. The PHV S RNA segment is 1675 nucleotides long. A long open reading frame was identified in the viral complementary-sense RNA that could encode a 433 amin...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90197-y

    authors: Parrington MA,Kang CY

    更新日期:1990-03-01 00:00:00

  • Temporal and spatial expression of the E5a protein during the differentiation-dependent life cycle of human papillomavirus type 31b.

    abstract::Human papillomaviruses (HPVs) are epitheliotropic viruses, and their life cycle is intimately linked to the stratification and differentiation state of the host epithelial tissues. Defining a role for the E5 gene product in the differentiation-dependent viral life cycle has been difficult due to the lack of a suitable...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9262

    authors: Mayer TJ,Meyers C

    更新日期:1998-09-01 00:00:00

  • HIV-1 Tat induces biochemical changes in the serum of mice.

    abstract::The HIV-1 Tat protein is released by infected cells and has numerous biological activities which might contribute either to the impairment of the immune response or to viral dissemination and pathogenesis. To date, the effects of Tat protein on metabolites remain unclear. In this study, a metabolomic study on serum of...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2011.11.005

    authors: Liao W,Tan G,Zhu Z,Chen Q,Lou Z,Dong X,Zhang W,Pan W,Chai Y

    更新日期:2012-01-20 00:00:00

  • Tomato spotted wilt Tospovirus genome reassortment and genome segment-specific adaptation.

    abstract::A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome se...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9131

    authors: Qiu WP,Geske SM,Hickey CM,Moyer JW

    更新日期:1998-04-25 00:00:00

  • Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization.

    abstract::In a previous paper (G. Winkler, V. B. Randolph, G. R. Cleaves, T. E. Ryan, and V. Stollar, 1988, Virology 162, 187-196) we showed that the newly synthesized dengue-2 virus nonstructural protein, NS1, exists briefly as a monomer and then undergoes dimerization. We demonstrate here that the dimerization of NS1 is assoc...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(89)90544-8

    authors: Winkler G,Maxwell SE,Ruemmler C,Stollar V

    更新日期:1989-07-01 00:00:00

  • Inhibition of Rb and p53 is insufficient for SV40 T-antigen transformation.

    abstract::The SV40 large T-antigen (TAg) has proven useful in studying pathways involved with cell division and tissue homeostasis. TAg disrupts the normal action of tumor suppressors pRb and p53. It is unclear whether T-antigen inhibition of p53 and pRb is sufficient for oncogenic transformation or if additional T-antigen acti...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2001.0866

    authors: Sachsenmeier KF,Pipas JM

    更新日期:2001-04-25 00:00:00

  • In vitro processing of human immunodeficiency virus type 1 Gag virus-like particles.

    abstract::Human immunodeficiency virus (HIV) Gag proteins are assembled into virus particles and then cleaved by the virion-associated HIV protease. Concomitant with Gag processing, doughnut-like HIV particles (the immature form) are converted to particles containing condensed cores (the mature form). Here we describe the in vi...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2000.0370

    authors: Morikawa Y,Shibuya M,Goto T,Sano K

    更新日期:2000-07-05 00:00:00

  • Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera.

    abstract::Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutraliz...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2012.12.014

    authors: Yu B,Dong J,Wang C,Zhan Y,Zhang H,Wu J,Kong W,Yu X

    更新日期:2013-03-15 00:00:00

  • Human Cytomegalovirus nuclear egress and secondary envelopment are negatively affected in the absence of cellular p53.

    abstract::Human Cytomegalovirus (HCMV) infection is compromised in cells lacking p53, a transcription factor that mediates cellular stress responses. In this study we have investigated compromised functional virion production in cells with p53 knocked out (p53KOs). Infectious center assays found most p53KOs released functional ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2016.07.021

    authors: Kuan MI,O'Dowd JM,Chughtai K,Hayman I,Brown CJ,Fortunato EA

    更新日期:2016-10-01 00:00:00

  • Antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus.

    abstract::Sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (HN) protein of a 1957 isolate of parainfluenza type 3 virus (PIV3) were produced and used to examine antigenic variation in clinical strains. Analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90395-2

    authors: van Wyke Coelingh KL,Winter C,Murphy BR

    更新日期:1985-06-01 00:00:00

  • Biosynthesis of reovirus-specified polypeptides. 2-aminopurine increases the efficiency of translation of reovirus s1 mRNA but not s4 mRNA in transfected cells.

    abstract::The effect of 2-aminopurine (2AP), an inhibitor of the RNA-dependent P1/eIF-2 protein kinase, on the expression of the reovirus serotype 1 Lang strain S1 and S4 genes in transfected simian COS cells was examined. In the absence of 2AP, the s4-encoded sigma 3 gene product was expressed about five times more efficiently...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90235-j

    authors: Samuel CE,Brody MS

    更新日期:1990-05-01 00:00:00

  • Functional similarities between HIV-1 Tat and DNA sequence-specific transcriptional activators.

    abstract::The Tat regulatory protein encoded by human immunodeficiency virus type 1 (HIV-1) induces high levels of transcription from the viral long terminal repeat (LTR) promoter element after interacting with a promoter proximal RNA target sequence. In the wild-type HIV-1 LTR, this activation is facilitated by the synergistic...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1041

    authors: Madore SJ,Cullen BR

    更新日期:1995-02-01 00:00:00

  • Host range of human T-cell leukemia virus type I analyzed by a cell fusion-dependent reporter gene activation assay.

    abstract::We constructed a sensitive and quantitative assay system to examine human T-cell leukemia virus type I (HTLV-I) envelope (env) glycoprotein-mediated cell fusion in which T7 RNA polymerase in donor cells coexpressing env glycoproteins activates a reporter gene in recipient cells upon cell fusion. An efficient expressio...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9530

    authors: Okuma K,Nakamura M,Nakano S,Niho Y,Matsuura Y

    更新日期:1999-02-15 00:00:00

  • Structural elements of primary CCR5-using HIV-1 gp120 proteins influencing sensitivity and resistance to the broadly neutralizing monoclonal antibody b12.

    abstract::Structure-guided approaches to HIV-1 vaccine design depend on knowledge of the presentation of neutralizing epitopes on gp120, such as the epitope for the broadly neutralizing mAb b12. Here, we characterized predicted three-dimensional structures of functionally diverse gp120 proteins in their b12-bound conformation, ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2012.06.024

    authors: Sterjovski J,Churchill MJ,Ellett A,Wesselingh SL,Ramsland PA,Gorry PR

    更新日期:2012-10-25 00:00:00

  • Expression of the 16K cistron of tobacco rattle virus in protoplasts.

    abstract::An antiserum was raised against a synthetic peptide corresponding to the 18 C-terminal amino acids of a putative 16K protein encoded by the 3'-terminal open reading frame of tobacco rattle virus (TRV) RNA-1. This antiserum was used to demonstrate expression of the 16K cistron in vivo. TRV-infected tobacco protoplasts ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(89)90155-4

    authors: Angenent GC,Verbeek HB,Bol JF

    更新日期:1989-04-01 00:00:00

  • Sequencing approach to analyze the role of quasispecies for classical swine fever.

    abstract::Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since R...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2012.11.020

    authors: Töpfer A,Höper D,Blome S,Beer M,Beerenwinkel N,Ruggli N,Leifer I

    更新日期:2013-03-30 00:00:00

  • Herpes simplex virus latent infection: reactivation and elimination of latency after neurectomy.

    abstract::Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(88)90085-2

    authors: Tenser RB,Edris WA,Hay KA

    更新日期:1988-11-01 00:00:00

  • A fused gene of nucleoprotein (NP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus.

    abstract::We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as ass...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2006.04.015

    authors: Saha S,Yoshida S,Ohba K,Matsui K,Matsuda T,Takeshita F,Umeda K,Tamura Y,Okuda K,Klinman D,Xin KQ,Okuda K

    更新日期:2006-10-10 00:00:00

  • Antigenic analyses of influenza virus haemagglutinins with different receptor-binding specificities.

    abstract::Monoclonal antibodies were used to compare the antigenicities of the haemagglutinins of two receptor binding mutants of X-31 (H3N2) influenza virus. The mutants which differed from each other in recognizing sialic acid in either alpha 2-6 linkage or alpha 2-3 linkage to galactose also differed exclusively at residue 2...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(84)90158-2

    authors: Daniels RS,Douglas AR,Skehel JJ,Wiley DC,Naeve CW,Webster RG,Rogers GN,Paulson JC

    更新日期:1984-10-15 00:00:00

  • Evidence for spontaneous circle formation in the replication of the satellite RNA of tobacco ringspot virus.

    abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(91)90005-v

    authors: Van Tol H,Buzayan JM,Bruening G

    更新日期:1991-01-01 00:00:00

  • Both homo and heterodimers of Marek's disease virus encoded Meq protein contribute to transformation of lymphocytes in chickens.

    abstract::Marek' disease virus serotype-1, also know as Gallid herpesvirus 2 (GaHV-2), elicits T-cell lymphomas in chickens. The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that Meq homodimers are not sufficient to induce lymphomas in chickens. In this s...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.01.006

    authors: Suchodolski PF,Izumiya Y,Lupiani B,Ajithdoss DK,Lee LF,Kung HJ,Reddy SM

    更新日期:2010-04-10 00:00:00

  • Evaluation of the zoonotic potential of multiple subgroups of clade 2.3.4.4 influenza A (H5N8) virus.

    abstract::Clade 2.3.4.4 H5N8 highly pathogenic avian influenza viruses (HPAIVs) have spread worldwide. Phylogenetic analysis identified two genetic groups of the H5N8 HPAIVs in South Korea; group A evolved further into four subgroups. Here, we examined the zoonotic potential, both in vivo and in vitro, of genetically distinct s...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2017.12.037

    authors: Lee YN,Lee EK,Song BM,Heo GB,Woo SH,Cheon SH,Lee YJ

    更新日期:2018-03-01 00:00:00

  • The structure of adeno-associated virus serotype 3B (AAV-3B): insights into receptor binding and immune evasion.

    abstract::Adeno-associated viruses (AAVs) are leading candidate vectors for human gene therapy. AAV serotypes have broad cellular tropism and use a variety of cellular receptors. AAV serotype 3 binds to heparan sulfate proteoglycan prior to cell entry and is serologically distinct from other serotypes. The capsid features that ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.03.027

    authors: Lerch TF,Xie Q,Chapman MS

    更新日期:2010-07-20 00:00:00

  • Nematode transmission of tobacco rattle virus serves as a bottleneck to clear the virus population from defective interfering RNAs.

    abstract::DI7 is a defective interfering RNA derived from RNA 2 of tobacco rattle tobravirus (TRV) isolate PpK20. Tobacco was transformed with DI7 cDNA fused to the CaMV 35S promoter. Upon infection of the transgenic plants with TRV isolate PpK20 or the serologically unrelated isolate PaY4, the transgenic DI7 RNA started to acc...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1999.9901

    authors: Visser PB,Brown DJ,Brederode FT,Bol JF

    更新日期:1999-10-10 00:00:00

  • Characterization and template properties of RNA dimers generated during flock house virus RNA replication.

    abstract::Flock house virus (FHV) is the best studied member of the Nodaviridae, a family of small, nonenveloped, isometric RNA viruses of insects and fish. Nodavirus genomes comprise two single-stranded positive-sense RNA segments (RNAs 1 and 2) that encode the viral RNA-dependent RNA polymerase (RdRp) and capsid protein precu...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2001.1125

    authors: Albariño CG,Price BD,Eckerle LD,Ball LA

    更新日期:2001-10-25 00:00:00