Abstract:
:Antibodies against the L protein of Sendai virus were made by immunizing rabbits with a synthetic peptide representing a carboxyl-terminal region of the protein predicted from the base sequence of its gene. These antibodies were used to localize the L protein in viral nucleocapsids by electron microscopy. Immunogold labeling revealed that L protein molecules were distributed in clusters along nucleocapsids, suggesting that L molecules act cooperatively in viral RNA synthesis. Immunogold double-labeling showed that all L clusters were associated with clusters of P molecules. We believe that this morphological association reflects the functional cooperation of the L and P proteins in viral RNA synthesis.
journal_name
Virologyjournal_title
Virologyauthors
Portner A,Murti KG,Morgan EM,Kingsbury DWdoi
10.1016/0042-6822(88)90257-7subject
Has Abstractpub_date
1988-03-01 00:00:00pages
236-9issue
1eissn
0042-6822issn
1096-0341journal_volume
163pub_type
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