当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > A variant of CRPV DNA preferentially maintained as a plasmid in NIH 3T3 cells and characterization of its transcripts in nude mouse tumors.

A variant of CRPV DNA preferentially maintained as a plasmid in NIH 3T3 cells and characterization of its transcripts in nude mouse tumors.

Abstract：

:Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CRPV DNA did not induce foci in NIH 3T3 cells and in cotransfections with a selectable marker only integrated DNA was found. The viral DNA appeared to be transcriptionally inactive since no transcripts could be detected and in cells were not tumorigenic for nude mice. In contrast to these results a spontaneously derived CRPV variant DNA was able to replicate extrachromosomally and a majority of focus-derived cell lines were tumorigenic for nude mice. In nude mouse tumors the variant DNA remained exclusively extrachromosomal and viral transcripts were detected. The sizes of the major transcripts were 2.0 and 1.3 kb and this suggested that E6 and E7 were expressed. The situation in rabbit tissue was different; both variant and wild-type DNA were maintained extrachromosomally. The extrachromosomal maintenance of the variant but not of wild-type DNA in mouse cells suggests that the variant lacks sequences which may play a role in the host and the tissue restriction of CRPV. The deletion in the variant DNA was located in the late region and included most or all of L1 and a carboxy terminal segment of L2. A second deletion eliminated some pBR322 sequences.

journal_name

Virology

journal_title

Virology

authors

Nasseri M,Wettstein FO

doi

10.1016/0042-6822(87)90149-8

subject

Has Abstract

pub_date

1987-12-01 00:00:00

pages

541-8

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

161

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • The C-X-C chemokine IP-10 stimulates HIV-1 replication.

    abstract：:Chemokines play critical roles in HIV-1 infection, serving both to modulate viral replication and to recruit target cells to sites of infection. Interferon-gamma-inducible protein 10 (IP-10/CXCL10) is a C-X-C chemokine that acts specifically upon activated T cells and macrophages and attracts T cells into the cerebros...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(02)00045-4

    authors： Lane BR,King SR,Bock PJ,Strieter RM,Coffey MJ,Markovitz DM

    更新日期：2003-03-01 00:00:00

  • Maintenance of human cytomegalovirus genome in human diploid fibroblast cells.

    abstract：:Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90139-3

    authors： Bucher LJ,Wigdahl B,Rapp F

    更新日期：1983-10-15 00:00:00

  • Cloning of bovine rotavirus (RF strain): nucleotide sequence of the gene coding for the major capsid protein.

    abstract：:The genes of the RF strain of bovine rotavirus have been cloned into pBR 322 following the synthesis and hybridization of cDNA transcribed from both strands of in vitro polyadenylated genomic RNA. Cloned rotavirus DNAs were assigned to most of the 11 genomic RNA segments by Northern blot hybridization. The complete se...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90159-4

    authors： Cohen J,Lefevre F,Estes MK,Bremont M

    更新日期：1984-10-15 00:00:00

  • Analysis of the SH3-binding region of HIV-1 nef: partial functional defects introduced by mutations in the polyproline helix and the hydrophobic pocket.

    abstract：:An SH3-binding domain within the Nef protein of primate lentiviruses has been reported to be important to viral replication and infectivity and dispensable for CD4 downregulation, but its precise role remains unclear. This study investigates the effects of mutations in both the polyproline helix and in the hydrophobic...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.9897

    authors： Craig HM,Pandori MW,Riggs NL,Richman DD,Guatelli JC

    更新日期：1999-09-15 00:00:00

  • The molecular biology of swinepox virus. II. The infectious cycle.

    abstract：:Studies based on low-stringency hybridizations of radiolabeled swinepox virus (SPV) DNA to Southern blots containing DNA of representative members of the Orthopoxvirus, Leporipoxvirus, and Avipoxvirus genera and the Entomopoxvirus subfamily have revealed no DNA homology at this level of resolution. Antigenic relatedne...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90040-i

    authors： Massung RF,Moyer RW

    更新日期：1991-01-01 00:00:00

  • DNA virus uncoating.

    abstract：:Virus genomes are condensed and packaged inside stable proteinaceous capsids that serve to protect them during transit from one cell or host organism, to the next. During virus entry, capsid shells are primed and disassembled in a complex, tightly-regulated, multi-step process termed uncoating. Here we compare the unc...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2015.01.024

    authors： Kilcher S,Mercer J

    更新日期：2015-05-01 00:00:00

  • Characterization of human adenovirus serotypes 5, 6, 11, and 35 as anticancer agents.

    abstract：:Human adenovirus type 5 (Ad5) has been the most popular platform for the development of oncolytic Ads. Alternative Ad serotypes with low seroprevalence might allow for improved anticancer efficacy in Ad5-immune patients. We studied the safety and efficacy of rare serotypes Ad6, Ad11 and Ad35. In vitro cytotoxicity of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.08.038

    authors： Shashkova EV,May SM,Barry MA

    更新日期：2009-11-25 00:00:00

  • Purification and characterization of human immunodeficiency virus type 1 nef gene product expressed by a recombinant baculovirus.

    abstract：:We have constructed the recombinant baculovirus which expresses the human immunodeficiency virus type 1 negative factor (nef) gene. Spodoptera frugiperda cells infected with the recombinant virus produced a 27-kDa protein which reacted with rabbit antisera raised against a carboxy-terminal synthetic peptide of the Nef...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90428-e

    authors： Matsuura Y,Maekawa M,Hattori S,Ikegami N,Hayashi A,Yamazaki S,Morita C,Takebe Y

    更新日期：1991-10-01 00:00:00

  • BST-2 mediated restriction of simian-human immunodeficiency virus.

    abstract：:Pathogenic simian-human immunodeficiency viruses (SHIV) contain HIV-1 Vpu and SIV Nef, both shown to counteract BST-2 (HM1.24; CD317; tetherin) inhibition of virus release in a species-specific manner. We show that human and pig-tailed BST-2 (ptBST-2) restrict SHIV. We found that sequential "humanization" of the trans...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.07.021

    authors： Ruiz A,Lau D,Mitchell RS,Hill MS,Schmitt K,Guatelli JC,Stephens EB

    更新日期：2010-10-25 00:00:00

  • Identification of putative polymerase gene product in cells infected with murine coronavirus A59.

    abstract：:The virion RNA of mouse hepatitis virus, strain A59 (MHV-A59) is believed to be the mRNA for the viral RNA-dependent RNA polymerase. The cell-free translation of virion RNA results in the synthesis of two predominant products p220 and p28 (M. R. Denison and S. Perlman, 1986, J. Virol. 60, 12-18). p28 is a basic protei...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90303-5

    authors： Denison M,Perlman S

    更新日期：1987-04-01 00:00:00

  • Identification of two independent neutralization domains on the VP4 trypsin cleavage products VP5* and VP8* of human rotavirus ST3.

    abstract：:The antigenic structure of the VP4 protein of human rotavirus (HRV) strains Wa and ST3 was studied by using a panel of Wa- and ST3-derived VP4-specific neutralizing monoclonal antibodies (NMAbs) and NMAb-resistant variants. The VP4-coding genes from three Wa and three ST3 variants were sequenced. For Wa VP4, one homot...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(95)80029-8

    authors： Padilla-Noriega L,Dunn SJ,López S,Greenberg HB,Arias CF

    更新日期：1995-01-10 00:00:00

  • Epitope mapping and use of epitope-specific antisera to characterize the VP5* binding site in rotavirus SA11 NSP4.

    abstract：:Rotavirus (RV) is the leading cause of infantile gastroenteritis worldwide. RV nonstructural protein 4 (NSP4), the first characterized viral enterotoxin, is a 28-kDa glycoprotein that has pleiotropic functions in RV infection and pathogenesis. NSP4 has multiple forms enabling it to perform its different functions. Dis...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.11.021

    authors： Hyser JM,Zeng CQ,Beharry Z,Palzkill T,Estes MK

    更新日期：2008-03-30 00:00:00

  • Differential effect of poly rI.rC and Newcastle disease virus on the expression of interferon and cellular genes in mouse cells.

    abstract：:The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90140-0

    authors： Kelley KA,Pitha PM

    更新日期：1985-12-01 00:00:00

  • Deep sequencing of Cotesia vestalis bracovirus reveals the complexity of a polydnavirus genome.

    abstract：:Here we completed the whole genome sequence of Cotesia vestalis bracovirus (CvBV) by deep sequencing and compared the genome features of CvBV to those of other polydnaviruses (PDVs). The genome is 540,215 base pairs divided into 35 genomic segments that range from 2.6 to 39.2kb. Comparison of CvBV with other PDVs show...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.03.009

    authors： Chen YF,Gao F,Ye XQ,Wei SJ,Shi M,Zheng HJ,Chen XX

    更新日期：2011-05-25 00:00:00

  • A novel method for obtaining poliovirus 14 S pentamers from procapsids and their self-assembly into virus-like shells.

    abstract：:Poliovirus procapsids, isolated and partially purified from infected HeLa cells, were found to dissociate into 14 S pentamers following freezing at -20 degrees overnight. Unlike pentameric subunits isolated directly from virus-infected cells or those obtained by alkaline treatment of procapsids, the "freeze-dissociate...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90465-x

    authors： Onodera S,Phillips BA

    更新日期：1987-08-01 00:00:00

  • Bacteriophage P22 capsid size determination: roles for the coat protein telokin-like domain and the scaffolding protein amino-terminus.

    abstract：:Assembly of icosahedral capsids of proper size and symmetry is not understood. Residue F170 in bacteriophage P22 coat protein is critical for conformational switching during assembly. Substitutions at this site cause assembly of tubes of hexamerically arranged coat protein. Intragenic suppressors of the ts phenotype o...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.06.025

    authors： Suhanovsky MM,Teschke CM

    更新日期：2011-09-01 00:00:00

  • The Helminthosporium victoriae 190S mycovirus has two forms distinguishable by capsid protein composition and phosphorylation state.

    abstract：:Purified preparations of the Helminthosporium victoriae 190S (Hv190S) virus contain two sedimenting components that differ in capsid structure. The slower sedimenting component (190S-1) contained p88 and p83 as the major capsid proteins; the faster component (190S-2) contained p88 and p78. Previous peptide-mapping stu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90520-y

    authors： Ghabrial SA,Havens WM

    更新日期：1992-06-01 00:00:00

  • Perturbation of DROSHA and DICER expression by human papillomavirus 16 oncoproteins.

    abstract：:Many tumors, including cervical carcinoma, show dysregulated expression of the microRNA processing machinery, specifically DROSHA and DICER. Some cervical cancers exhibit chromosome 5p amplifications and DROSHA is the most significantly upregulated transcript and is observed in all tumors with 5p gain. DROSHA and DICE...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.04.022

    authors： Harden ME,Munger K

    更新日期：2017-07-01 00:00:00

  • Characterization of multiple molecular interactions between human cytomegalovirus (HCMV) and human immunodeficiency virus type 1 (HIV-1).

    abstract：:In transient gene expression assays we observed an increase in expression of the bacterial chloramphenicol acetyl-transferase (CAT) gene, under the transcriptional control of the HIV-1 LTR (pLTR-CAT), when this plasmid was cotransfected into Vero or MRC-5 cells with a plasmid containing either the HCMV immediate early...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90233-h

    authors： Rando RF,Srinivasan A,Feingold J,Gonczol E,Plotkin S

    更新日期：1990-05-01 00:00:00

  • Binding to CD4 of synthetic peptides patterned on the principal neutralizing domain of the HIV-1 envelope protein.

    abstract：:The interaction between the viral envelope protein gp120 and the cellular surface antigen CD4 is a key event in HIV-1 infection. Reciprocal high affinity binding sites have been located in the first domain of CD4 and in the carboxy-terminal region of gp120, respectively. Upon infection, the membranes of the target cel...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90553-n

    authors： Autiero M,Abrescia P,Dettin M,Di Bello C,Guardiola J

    更新日期：1991-12-01 00:00:00

  • Use of a high-affinity peptide that aborts MHC-restricted cytotoxic T lymphocyte activity against multiple viruses in vitro and virus-induced immunopathologic disease in vivo.

    abstract：:Binding of a specific peptide(s) from a viral protein to major histocompatibility complex (MHC) class I molecules is a critical step in the activation of CD8(+) cytotoxic T lymphocytes (CTLs). Once activated, CTLs can cause lethal disease in an infected host, for example, by killing virus-containing ependymal and vent...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9593

    authors： Oldstone MB,von Herrath M,Lewicki H,Hudrisier D,Whitton JL,Gairin JE

    更新日期：1999-04-10 00:00:00

  • Cloning and molecular characterization of an oral papillomavirus of domestic rabbits.

    abstract：:DNA obtained from New Zealand white rabbit oral papillomas was analyzed for the presence of papillomavirus DNA. The viral genome was cloned as three separate subclones, which were each mapped and oriented with respect to one another. Comparisons with other papillomavirus DNAs by Southern blot hybridization under vario...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(88)90411-4

    authors： O'Banion MK,Cialkowski ME,Reichmann ME,Sundberg JP

    更新日期：1988-01-01 00:00:00

  • Characterization of the leader papain-like proteinase of MHV-A59: identification of a new in vitro cleavage site.

    abstract：:Sequence analysis of the mouse hepatitis virus, strain A59 (MHV-A59) genome predicts the presence of two papain-like proteinases encoded within the first open reading frame (ORF 1a) of the replicase gene. The more 5' of these domains, the leader papain-like proteinase, is responsible for the cleavage of the amino term...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1281

    authors： Bonilla PJ,Hughes SA,Piñón JD,Weiss SR

    更新日期：1995-06-01 00:00:00

  • Development of reassortant viruses between pathogenic hantavirus strains.

    abstract：:Segment reassortment of negative strand viruses is an important mechanism for the development of new virus strains with altered pathogenicity. This study reports on in vitro generation of reassortants between Andes (ANDV) and Sin Nombre (SNV) viruses. Although they both cause hantavirus pulmonary syndrome (HPS), ANDV ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2004.07.012

    authors： Rizvanov AA,Khaiboullina SF,St Jeor S

    更新日期：2004-10-01 00:00:00

  • Nucleotide and amino acid sequence analysis of the rotavirus nonstructural RNA-binding protein NS35.

    abstract：:NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1059

    authors： Patton JT,Salter-Cid L,Kalbach A,Mansell EA,Kattoura M

    更新日期：1993-02-01 00:00:00

  • Human papillomavirus types 16 E6 and E7 contribute differently to carcinogenesis.

    abstract：:High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0106

    authors： Song S,Liem A,Miller JA,Lambert PF

    更新日期：2000-02-15 00:00:00

  • Molecular characterization of Drosophila cells persistently infected with Flock House virus.

    abstract：:Little is known about the molecular determinants causing and sustaining viral persistent infections at the cellular level. We found that Drosophila cells persistently infected (PI) with Flock House virus (FHV) invariably harbor defective viral RNAs, which are replicated by the FHV RNA-dependent RNA polymerase. Some de...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.08.002

    authors： Jovel J,Schneemann A

    更新日期：2011-10-10 00:00:00

  • Autographa californica multiple nucleopolyhedrovirus core gene ac92 (p33) is required for efficient budded virus production.

    abstract：:Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac92 is a core gene encoding a protein associated with occlusion derived virus (ODV), binds human P53 and also has flavin adenine dinucleotide linked sulfhydryl oxidase activity but its role in the virus life cycle is not known. To determine ac92 function a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.09.023

    authors： Nie Y,Fang M,Theilmann DA

    更新日期：2011-01-05 00:00:00

  • Interviral homologies of the 30K proteins of tobamoviruses.

    abstract：:The 30K protein of tobacco mosaic virus was recently confirmed to be involved in cell-to-cell movement of the virus. To characterize common structural features of the 30K protein, the nucleotide sequence of the 30K protein gene of cucumber green mottle mosaic virus (CGMMV, a member of the tobamoviruses) RNA has been d...

    journal_title：Virology

    pub_type： 杂志文章

    doi：

    authors： Saito T,Imai Y,Meshi T,Okada Y

    更新日期：1988-12-01 00:00:00

  • The morphogenetic signal of bacteriophage f1.

    abstract：:The genome of the single-stranded DNA phage f1 contains an intergenic region (IG), 508-nucleotides long, that does not code for any known protein. By use of a system of chimeric plasmids haboring different f1 fragments, we had previously shown that this region contains, in addition to the f1 'functional origin' of DNA...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90136-8

    authors： Dotto GP,Zinder ND

    更新日期：1983-10-15 00:00:00