Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization.

abstract：:Persistent infection with the Edmonston strain of measles virus (MV) has been established in IC-21 cells, an immortalized murine macrophage cell line. Persistence was established immediately without syncytia formation or cytopathic effects. MV was expressed in the majority of the cells as evidenced by immunofluorescen...

journal_title：Virology

authors： Goldman MB,Buckthal DJ,Picciotto S,O'Bryan TA,Goldman JN

更新日期：1995-02-20 00:00:00

abstract：:The coding region of segment 4 of Thogoto (THO) virus, a tick-borne member of the Orthomyxoviridae, was expressed in a baculovirus system under the control of the polyhedrin promoter. This construct expressed authentic envelope glycoprotein as determined by size and antigenic reactivity with a panel of monoclonal anti...

journal_title：Virology

authors： Jones LD,Morse MA,Marriott AC,Nuttall PA

更新日期：1995-10-20 00:00:00

abstract：:Hepatitis C virus (HCV) core protein constitutes a viral nucleocapsid and may possess multiple functions. In this study, we demonstrated the homotypic interaction and multimerization of HCV core protein in vitro and in vivo. By using a yeast two-hybrid system, we showed that the amino-terminal hydrophilic portion (ami...

journal_title：Virology

authors： Matsumoto M,Hwang SB,Jeng KS,Zhu N,Lai MM

更新日期：1996-04-01 00:00:00

abstract：:The lentivirus caprine arthritis-encephalitis virus (CAEV) is closely related by nucleotide sequence homology to visna virus and other sheep lentiviruses and shows less similarity to the other animal and human lentiviruses. The genomic organization of CAEV is very similar to that of visna virus and the South African o...

journal_title：Virology

authors： Saltarelli M,Querat G,Konings DA,Vigne R,Clements JE

更新日期：1990-11-01 00:00:00

abstract：:Functions of the coat protein of white clover mosaic potexvirus (WCIMV) were investigated using C-terminal deletion mutants. Whereas plants inoculated with RNA transcripts of a full-length wild-type clone of WCIMV produced typical infections, plants inoculated with transcripts of each mutant did not produce symptoms, ...

journal_title：Virology

authors： Forster RL,Beck DL,Guilford PJ,Voot DM,Van Dolleweerd CJ,Andersen MT

更新日期：1992-11-01 00:00:00

abstract：:Crosslinking reagents were used to interrupt the process of filamentous phage morphogenesis and investigate the orientation in which nascent virions are extruded through the host cell membrane. Infected bacteria with emerging phage particles were crosslinked with glutaraldehyde. Immunoferritin-labeling studies on thes...

journal_title：Virology

authors： Lopez J,Webster RE

更新日期：1983-05-01 00:00:00

abstract：:In benign squamous lesions and in organotypic epithelial cultures, the human papillomavirus (HPV) E6 and E7 genes are transcriptionally up-regulated in differentiated, spinous keratinocytes. We previously identified sequence elements in the enhancer-promoter regions of HPV types 18 and 11 important for this promoter r...

journal_title：Virology

authors： Zhao W,Chow LT,Broker TR

更新日期：1999-01-20 00:00:00

abstract：:The heptauridine tract at each gene end and intergenic region (IGR) at the gene junctions of vesicular stomatitis virus (VSV) have effects on synthesis of the downstream mRNA, independent of their respective roles in termination of the upstream mRNA. To investigate the role of the U tract and the IGR in downstream gen...

journal_title：Virology

authors： Hinzman EE,Barr JN,Wertz GW

更新日期：2008-10-25 00:00:00

abstract：:The cowpea strain of Southern bean mosaic virus (SBMV-C), a T = 3 icosahedral RNA virus, was dissociated to yield a ribonucleoprotein complex (RNPC) composed of the viral RNA and coat protein subunits. To determine if the coat protein subunits were bound to a specific site on the viral RNA, the RNPC was treated with r...

journal_title：Virology

authors： Hacker DL

更新日期：1995-03-10 00:00:00

abstract：:The sequence of gene VI from figwort mosaic virus (FMV) clone x4 was determined and compared with that previously published for FMV clone DxS. Both clones originated from the same virus isolation, but the virus used to clone DxS was propagated extensively in a host of a different family prior to cloning whereas that u...

journal_title：Virology

authors： Sanger M,Daubert S,Goodman RM

更新日期：1991-06-01 00:00:00

abstract：:Tetherin has been defined as a restriction factor of HIV-1 and several other enveloped viruses. However, the significance of tetherin in viral infection remains to be further addressed. Here, we investigated whether tetherin plays a role in HSV-2 infection. Our study revealed that overexpression of tetherin restricted...

journal_title：Virology

authors： Liu Y,Luo S,He S,Zhang M,Wang P,Li C,Huang W,Hu B,Griffin GE,Shattock RJ,Hu Q

更新日期：2015-01-15 00:00:00

abstract：:The simian virus 40 (SV40) large T antigen was used as an immunogenic vector to express a herpes simplex virus type 1 (HSV-1) glycoprotein B (gB), H-2Kb-restricted cytotoxic T lymphocyte (CTL) recognition epitope corresponding to amino acid residues 498-505. Immunization of naive, C57BL/6 mice with a cell line, B6/350...

journal_title：Virology

authors： Bonneau RH,Fu TM,Tevethia SS

更新日期：1993-12-01 00:00:00

abstract：:The phosphatidylinositol-3-kinase (PI3K)-Akt signaling pathway regulates several key cellular functions including protein synthesis, cell growth, glucose metabolism, and inflammation. Many viruses have evolved mechanisms to manipulate this signaling pathway to ensure successful virus replication. The human herpesvirus...

journal_title：Virology

authors： Liu X,Cohen JI

更新日期：2015-05-01 00:00:00

abstract：:Guinea pig cytomegalovirus (GPCMV) immediate early proteins, IE1 and IE2, demonstrated structural and functional homologies with human cytomegalovirus (HCMV). GPCMV IE1 and IE2 co-localized in the nucleus with each other, the viral polymerase and guinea pig ND10 components (gpPML, gpDaxx, gpSp100, gpATRX). IE1 showed ...

journal_title：Virology

authors： Hornig J,Choi KY,McGregor A

更新日期：2017-04-01 00:00:00

abstract：:ICP27 is an essential herpes simplex virus type 1 (HSV-1) nuclear protein which regulates viral early and late genes during infection. The exact mechanism by which ICP27 modulates viral gene expression is unknown, but considerable evidence suggests that it functions posttranscriptionally. In this study, we have asked ...

journal_title：Virology

authors： Mears WE,Rice SA

更新日期：1998-03-01 00:00:00

abstract：:Pseudorecombinants produced by exchanging genome components (DNAs A and B) of the geminiviruses African cassava mosaic virus (ACMV) and Indian cassava mosaic virus (ICMV), ACMV, and tomato golden mosaic virus (TGMV), and TGMV and abutilon mosaic virus (AbMV) are not infectious in their common host Nicotiana benthamian...

journal_title：Virology

authors： Frischmuth T,Roberts S,von Arnim A,Stanley J

更新日期：1993-10-01 00:00:00

abstract：:We cloned the complete complementary DNA of an isolate of the hepatitis C virus, HCV-S1, into a tetracycline-inducible expression vector and stably transfected it into two human hepatoma cell lines, Huh7 and HepG2. Twenty-six Huh7 and two HepG2-positive clones were obtained after preliminary screening. Two Huh7 (SH-7 ...

journal_title：Virology

authors： Lim SP,Soo HM,Tan YH,Brenner S,Horstmann H,MacKenzie JM,Ng ML,Lim SG,Hong Wj

更新日期：2002-11-10 00:00:00

abstract：:Old world hantaviruses, causing hemorrhagic fever with renal syndrome (HFRS), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. T...

journal_title：Virology

authors： Koch J,Liang M,Queitsch I,Kraus AA,Bautz EK

更新日期：2003-03-30 00:00:00

abstract：:Natural infection and vaccination with a live-attenuated measles virus (MV) induce CD8(+) T-cell-mediated immune responses that may play a central role in controlling MV infection. In this study, we show that newly identified human HLA-A2 epitopes from MV hemagglutinin (H) and fusion (F) proteins induced protective im...

journal_title：Virology

authors： Oh S,Stegman B,Pendleton CD,Ota MO,Pan CH,Griffin DE,Burke DS,Berzofsky JA

更新日期：2006-09-01 00:00:00

abstract：:The molecular host response to hepatitis C virus (HCV) infection was examined by isolation of HCV-induced genes from a cDNA library constructed from chimpanzee liver during the acute phase of hepatitis C. Two cDNA clones, 130-7 and 130-51, were obtained by differential hybridization with cDNA probes prepared from poly...

journal_title：Virology

authors： Kato T,Esumi M,Yamashita S,Abe K,Shikata T

更新日期：1992-10-01 00:00:00

abstract：:Marek's disease (MD) infection has been controlled effectively by vaccination using nononcogenic and/or attenuated oncogenic Marek's disease virus (MDV) vaccines. Thus far, there is little knowledge on the role of cell-mediated immune (CMI) responses during MDV infection or vaccination. To elucidate the importance of ...

journal_title：Virology

authors： Omar AR,Schat KA

更新日期：1996-08-01 00:00:00

abstract：:Bacteriophage P2 integrase (Int) mediates site-specific recombination leading to integration or excision of the phage genome in or out of the bacterial chromosome. Int belongs to the large family of tyrosine recombinases that have two different DNA recognition motifs binding to the arm and core sites, respectively, wh...

journal_title：Virology

authors： Frumerie C,Sylwan L,Ahlgren-Berg A,Haggård-Ljungquist E

更新日期：2005-02-05 00:00:00

abstract：:The HIV-1 Nef protein causes a decrease in major histocompatibility complex (MHC) class I and CD4 molecule expression on the cell surface. To determine if Nef can affect components of the innate immune response, we assessed the ability of Nef to alter the cell surface expression of human CD1d. In cells co-expressing C...

journal_title：Virology

authors： Cho S,Knox KS,Kohli LM,He JJ,Exley MA,Wilson SB,Brutkiewicz RR

更新日期：2005-07-05 00:00:00

abstract：:The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subun...

journal_title：Virology

authors： Annamalai P,Rao AL

更新日期：2005-02-20 00:00:00

abstract：:An open reading frame which encodes at least 90% of the adenovirus type 2 DNA polymerase gene was cloned behind the SP6 promoter and transcribed in vitro using the SP6 RNA polymerase. The resultant RNA was translated in a rabbit reticulocyte cell free system. In addition to the translation of a 120-kDa protein corresp...

journal_title：Virology

authors： Hassin D,Korn R,Horwitz MS

更新日期：1986-11-01 00:00:00

abstract：:Latent membrane protein 2A (LMP2A) is expressed in latent Epstein-Barr virus (EBV) infection. LMP2A functions to downregulate B-cell signal transduction and viral reactivation from latency in EBV-immortalized B cells in vitro, and acts to provide B cells with both a survival and developmental signal in vivo. Identific...

journal_title：Virology

authors： Ikeda M,Ikeda A,Longan LC,Longnecker R

更新日期：2000-03-01 00:00:00

abstract：:West Nile virus (WNV) is a mosquito-borne flavivirus that causes febrile illness, encephalitis, and occasionally death in humans. The envelope protein is the main component of the WNV virion surface, and domain III of the envelope protein (EIII) is both a putative receptor binding domain and a target of highly specifi...

journal_title：Virology

authors： Plante JA,Torres M,Huang CY,Beasley DWC

更新日期：2016-09-01 00:00:00

abstract：:The propensity of retroviruses to rapidly establish persistent infections poses a formidable problem in vaccination strategies. In the current study, we use a live attenuated vaccine to study protection against acute and persistent Friend virus infections in mice. Adoptive transfers of immune CD8(+) T cells combined w...

journal_title：Virology

authors： Dittmer U,Hasenkrug KJ

更新日期：2000-06-20 00:00:00

abstract：:The genes of the RF strain of bovine rotavirus have been cloned into pBR 322 following the synthesis and hybridization of cDNA transcribed from both strands of in vitro polyadenylated genomic RNA. Cloned rotavirus DNAs were assigned to most of the 11 genomic RNA segments by Northern blot hybridization. The complete se...

journal_title：Virology

authors： Cohen J,Lefevre F,Estes MK,Bremont M

更新日期：1984-10-15 00:00:00

abstract：:Monoclonal antibodies (MAb) against an O1 Suisse isolate of FMDV were used to identify epitopes on the virus particle and to determine their relative function. Six major antigenic sites containing one or more epitopes were identified using competition ELISA. An epitope relationship is proposed consisting of a trypsin-...

journal_title：Virology

authors： McCullough KC,Crowther JR,Carpenter WC,Brocchi E,Capucci L,De Simone F,Xie Q,McCahon D

更新日期：1987-04-01 00:00:00