Abstract:
:Coat protein-protected fragments of alfalfa mosaic virus RNA (AlMV-RNA) and tobacco streak virus RNA (TSV-RNA), which were isolated as described [D. Zuidema, M. F. A. Bierhuizen, B. J. C. Cornelissen, J. F. Bol, and E. M. J. Jaspars (1983)Virology, 125, 361-369], were tested for their ability to rebind AlMV coat protein in the presence of an excess of Escherichia coli tRNA by means of a nitrocellulose filter retention assay. In order to obtain the minimum requirements for coat protein binding, a 3'-terminal binding site and several internal binding sites were isolated and fragmented by mild alkali treatment so that various lengths of a particular binding site were present in the mixture to be tested for rebinding capacity. All fragments which originated from the Wend of AlMV-RNA 1 and could bind AlMV coat protein have in common the sequence 5'-CUCAUGCUA-3'. However, this sequence alone is not sufficient to bind viral coat protein. Either an extension by at least 27 nucleotides of this oligomer to the right or an extension by 45 nucleotides (or possibly less) to the left is necessary for AlMV coat protein binding. Also, smaller extensions simultaneously occurring at both sides are sufficient. The smallest fragment which still has binding capacity for viral coat protein is 23 nucleotides long and originates from an internal site of RNA 1. All bound fragments have two common features: the occurrence of AUG(C) twice in the sequence and the potential ability to form a stable secondary structure. A striking observation was that 3'-terminal fragments of TSV-RNAs 1 and 2 rebind AlMV coat protein with low efficiency (about 27 and 37%, respectively), whereas a 3'-terminal fragment of TSV-RNA 3 rebinds AlMV coat protein with an efficiency of about 71%.
journal_name
Virologyjournal_title
Virologyauthors
Zuidema D,Cool RH,Jaspars EMdoi
10.1016/0042-6822(84)90165-xsubject
Has Abstractpub_date
1984-07-30 00:00:00pages
282-92issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(84)90165-Xjournal_volume
136pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The nucleotide sequences of the long terminal repeat (LTR) of simian retroviruses (STLV), which are closely related to human T-cell leukemia virus type I (HTLV-I) were found to be of at least two subgroups: an Asian subtype in macaques and an African subtype in African green monkeys and chimpanzee. The nucleotide sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90336-3
更新日期:1986-01-30 00:00:00
abstract::Viruses of the Norovirus genus (Caliciviridae family) are a major cause of human gastroenteritis. In some viruses, recombination is an important evolutionary process and therefore we should try to discover the quantity and characteristics of such events in Noroviruses. In order to identify recombination events, multip...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.051
更新日期:2006-02-05 00:00:00
abstract::Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1069
更新日期:1994-02-01 00:00:00
abstract::Cultured primary peripheral blood mononuclear cells (PBMC) represent a potentially physiologic in vitro model of HIV-1 infection, but assessment of antibody-mediated HIV-1 neutralization using PBMC has been hindered by donor variability and lack of a sustainable individual PBMC source. To advance this model for HIV va...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.09.014
更新日期:2013-12-01 00:00:00
abstract::Enterovirus 71 (EV71) is a major cause of hand-foot-and-mouth disease. EV71 infection occasionally associates with severe neurological sequelae such as brainstem encephalitis or poliovirus-like paralysis. We demonstrated that mouse-adapted strain increases infectivity, resulting in higher cytotoxicity of neuron cells ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.10.015
更新日期:2012-01-05 00:00:00
abstract::The location of nucleotide sequences important in determining the extent of cowpea mosaic virus M-RNA accumulation in cowpea protoplasts has been analyzed by deletion mutagenesis of full-length cDNA clones from which infectious transcripts can be produced in vitro. The results suggest that cis-acting sequences which d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1175
更新日期:1993-04-01 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.09.006
更新日期:2021-01-02 00:00:00
abstract::Despite recent progress in the development of direct-acting antivirals against hepatitis C virus (HCV), chronic HCV infection remains an important health burden worldwide. MicroRNA122 (miR122), a liver-specific microRNA (miRNA), positively regulates HCV replication, and systemic application of antisense oligonucleotid...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.024
更新日期:2014-08-01 00:00:00
abstract::In the event of smallpox bioterrorism, widespread vaccination may be required. Vaccinia immune globulin (VIG) has been used to treat complications from the smallpox vaccine. While the potency of VIG was defined by its ability to neutralize intracellular mature virus, a second form of vaccinia called the extracellular ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.004
更新日期:2004-08-01 00:00:00
abstract::Most adenoviral vectors use in gene therapy protocols derive from species C. However, expression of the primary receptor (human Coxsackievirus and Adenovirus receptor, hCAR) for these AdV is variable on cancer cells. In vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therap...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.12.023
更新日期:2004-04-10 00:00:00
abstract::We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8648
更新日期:1997-08-04 00:00:00
abstract::Pseudorecombinants produced by exchanging genome components (DNAs A and B) of the geminiviruses African cassava mosaic virus (ACMV) and Indian cassava mosaic virus (ICMV), ACMV, and tomato golden mosaic virus (TGMV), and TGMV and abutilon mosaic virus (AbMV) are not infectious in their common host Nicotiana benthamian...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1523
更新日期:1993-10-01 00:00:00
abstract::Crosslinking reagents were used to interrupt the process of filamentous phage morphogenesis and investigate the orientation in which nascent virions are extruded through the host cell membrane. Infected bacteria with emerging phage particles were crosslinked with glutaraldehyde. Immunoferritin-labeling studies on thes...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90382-3
更新日期:1983-05-01 00:00:00
abstract::The glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) serves as virus attachment protein to its receptor on host cells and is a key determinant for cell tropism, pathogenesis, and epidemiology of the virus. The GP of LCMV is posttranslationally cleaved by the subtilase SKI-1/S1P into two subunits, the per...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00421-5
更新日期:2003-09-15 00:00:00
abstract::We measured the effects of non-nucleoside reverse transcriptase (RT) inhibitor-resistant mutations K101E+G190S, on replication fitness and EFV-resistance of HIV(NL4-3). K101E+G190S reduced fitness in the absence of EFV and increased EFV resistance, compared to either single mutant. Unexpectedly, K101E+G190S also repli...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.018
更新日期:2010-07-05 00:00:00
abstract::Previous reports from our lab had shown that sera obtained from SIV(mac)-infected animals neutralized SIV(mac) infectivity in CD4(+) T cells but failed to protect monkey primary macrophages from infection with the virus. However, the antibodies could inhibit completion of the viral life cycle in the macrophages at the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1053
更新日期:2001-09-01 00:00:00
abstract::Gene transfer into human hematopoietic stem cells using Ad5 is inefficient due to lack of the primary receptor CAR and the secondary receptors alphavbeta3 integrin and alphavbeta5 integrin, and due to the high seroprevalence of Ad5 antibodies in most adults, resulting in diminished gene transduction. In the present st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.07.018
更新日期:2004-10-25 00:00:00
abstract::The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.03.037
更新日期:2005-06-20 00:00:00
abstract::The recent development of arterivirus full-length cDNA clones makes possible the construction of chimeric arteriviruses for fundamental and applied studies. Using an equine arteritis virus (EAV) infectious cDNA clone, we have engineered chimeras in which the ectodomains of the two major envelope proteins, the glycopro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1074
更新日期:2001-09-30 00:00:00
abstract::Attenuated mutants of Venezuelan equine encephalitis virus (VEE) were isolated by selection for rapid penetration of cultured cells (R. E. Johnston and J. F. Smith, 1988, Virology 162, 437-443). Sequence analysis of these mutants identified candidate attenuating mutations at four loci in the VEE E2 glycoprotein gene: ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90114-q
更新日期:1991-07-01 00:00:00
abstract::Large numbers of VSV (LCMV) pseudotypes with the genomes of vesicular stomatitis virus (VSV) and the coat proteins of lymphocytic choriomeningitis virus (LCMV) were produced by infecting L cells first with LCMV and subsequently with VSV, the latter in the presence of tunicamycin. Separation by gradient centrifugation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90007-2
更新日期:1984-08-01 00:00:00
abstract::The nucleotide sequence of the gene immediately following the nucleocapsid protein gene of sonchus yellow net virus (SYNV), a plant rhabdovirus, is presented. Serological reactions of SYNV proteins with antibodies elicited by a fusion protein constructed from the sequenced gene indicate that this gene encodes an SYNV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90190-5
更新日期:1987-11-01 00:00:00
abstract::L83V-related variants of human papillomavirus (HPV) 16 E6, exemplified by the Asian-American variant Q14H/H78Y/L83V, were shown to be more prevalent than E6 prototype in progressing lesions and cervical cancer. We evaluated functions relevant to carcinogenesis for the E6 variants L83V, R10/L83V and Q14H/H78Y/L83V as w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.036
更新日期:2009-01-05 00:00:00
abstract::Alphaherpesviruses comprise closely related viruses of man and animal, including herpes simplex virus, varicella-zoster virus and pseudorabies virus (PRV). Here, using methyl-beta-cyclodextrin and fluorescently tagged PRV, we directly show that depletion of cholesterol from the plasma membrane of host cells significan...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.039
更新日期:2008-07-05 00:00:00
abstract::To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.011
更新日期:2015-11-01 00:00:00
abstract::The amino acid sequence of BMV (brome mosaic virus) coat protein is presented and compared with the sequence derived from RNA structure. Apart from removal of the N-terminal methionine residue and acetylation of the following serine residue, no post-translational modifications occur. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90193-9
更新日期:1983-09-01 00:00:00
abstract::The nucleotide and deduced amino acid sequence of the gene 4 of bovine rotavirus strain B223 is described. The open reading frame is predicted to encode a VP4 of 772 amino acids, shorter than described for any other rotavirus strain sequenced to date. B223 VP4 shows 70 to 73% similarity to other rotavirus VP4 proteins...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90191-q
更新日期:1992-11-01 00:00:00
abstract::Three distinct species of nucleocapsids of equine herpesvirus type-1, designated as either light (L), intermediate (I), or heavy (H) on the basis of their densities in Renografin-76 density gradients (Perdue et al. 1975), were characterized with respect to their amino acid content, DNA content, and role in the maturat...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
abstract::cDNA clones of the St. Louis encephalitis (SLE) virus genome have been obtained and the nucleotide sequence of 4.7 kb corresponding to the 5' terminal half of the genome determined. The genome contains a 5' noncoding region of 98 nucleotides followed by a single continuous open reading frame that encodes three structu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90409-0
更新日期:1987-02-01 00:00:00
abstract::RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9497
更新日期:1999-01-05 00:00:00